食品与发酵工业 >

2021 , Vol. 47 >Issue 14: 151 - 159

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.025762

生产与科研应用

响应面试验优化超声波辅助双水相提取蕨麻多糖

  • 郭杰 ,
  • 陶蕾 ,
  • 王吉鸿 ,
  • 王瑞雪
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  • (兰州职业技术学院 生物工程系,甘肃 兰州,730070)
博士研究生,讲师(本文通讯作者,E-mail:crystal_g@foxmail.com)

收稿日期: 2020-09-26

  修回日期: 2021-01-04

  网络出版日期: 2021-08-20

基金资助

国家自然科学基金资助项目(51873175);2020年度兰州职业技术学院校内项目(2020XY-8);兰州市科技发展指导性计划项目(2019-ZD-153)

收起

Optimization of ultrasonic-assisted aqueous two-phase extraction of polysaccharides from Potentilla anserina L.using response surface methodology

  • GUO Jie ,
  • TAO Lei ,
  • WANG Jihong ,
  • WANG Ruixue
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  • (Bioengineering Department, Lanzhou Vocational Technical College, Lanzhou 730070, China)

Received date: 2020-09-26

  Revised date: 2021-01-04

  Online published: 2021-08-20

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摘要

应用单因素试验和响应面法优化超声波辅助双水相提取蕨麻多糖工艺。在单因素试验的基础上,利用Box-Behnken design模型对提取参数无水乙醇质量分数、硫酸铵质量分数、料液比和超声功率进行了优化,选取最优条件,并通过红外色谱、气相色谱质谱联用技术初步探讨蕨麻多糖的化学结构,体外活性实验研究其生物活性。结果显示,超声波辅助双水相提取蕨麻多糖最佳工艺条件在乙醇质量分数为31%,硫酸铵质量分数19%,料液比1∶22(g∶mL),超声功率240 W的条件下,蕨麻多糖提取率达到(9.04±0.12)％。红外光谱分析证实提取产物为多糖物质,气相色谱质谱联用技术分析蕨麻多糖是由鼠李糖、甘露糖、葡萄糖和半乳糖组成,其摩尔比为1.00∶1.69∶10.61∶2.66。体外活性实验表明,在测试范围内超声波辅助双水相法提取的蕨麻多糖抗氧化活性优于传统水提蕨麻多糖。超声波辅助双水相技术是一种可靠高效的多糖提取方法。

关键词： 响应面法; 超声波; 双水相; 蕨麻; 多糖

本文引用格式

郭杰 , 陶蕾 , 王吉鸿 , 王瑞雪 . 响应面试验优化超声波辅助双水相提取蕨麻多糖[J]. 食品与发酵工业, 2021 , 47(14) : 151 -159 . DOI: 10.13995/j.cnki.11-1802/ts.025762

Abstract

Single-factor experiment and response surface methodology were used to optimize the ultrasonic-assisted aqueous two-phase extraction of polysaccharides from Potentilla anserina L. Four key factors including ethanol concentration, ammonium sulfate concentration, solid-liquid ratio and ultrasonic power were selected by one-factor-at-a-time experiments. The optimum conditions of ultrasonic-assisted aqueous two-phase extraction of polysaccharides from Potentilla anserina L. were as follows: Ultrasonic power at 240 W with 31% ethanol volume and 19% ammonium sulfate, the extraction yield of polysaccharides from Potentilla anserina L. reached (9.035±0.119)%. Infrared spectrum analysis confirmed that the extracts were polysaccharides. Potentilla anserina polysaccharide was composed of rhamnose, mannose, glucose and galactose, with the molar ratio of 1.00∶1.69∶10.61∶2.66. The in vitro activity results showed that the antioxidant activity of polysaccharides extracted by ultrasonic-assisted two-phase extraction was better than that of traditional water extraction. Ultrasonic assisted aqueous two-phase technology was a reliable and efficient method for polysaccharide extraction.

Key words： response surface methodology; ultrasonic; aqueous two-phase extraction; Potentilla anserina L.; polysaccharides

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