为评价发酵火麻仁蛋白粉的抗氧化能力,利用偶氮二异丁基二盐酸盐(azodiisobutyl dicarbonate,AAPH)诱导HepG2细胞建立氧化应激模型,通过测定细胞内活性氧(reactive oxygen species,ROS)自由基、丙二醛(malondialdehyde,MDA)含量以及超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)的活力,评价发酵火麻仁蛋白粉的抗氧化活性;并且对HepG2细胞抗氧化相关基因的表达进行研究。结果表明,发酵火麻仁蛋白粉显著增加SOD、GSH-Px活力(P<0.05),降低胞内ROS含量与MDA含量,发酵火麻仁粉抗氧化酶基因NQO1、Nrf2和HO-1 mRNA表达水平增加,表明发酵火麻仁蛋白粉能够激活Nrf2通路,减轻AAPH诱导的HepG2氧化应激损伤作用。
To evaluate the antioxidant capacity of fermented hemp seed protein powder, oxidative stress of HepG2 cells was induced by 2,2′-azobis(2-methylpropionamidine)dihydrochloride (AAPH) to establish the model. The antioxidant activity was measured by reactive oxygen species (ROS), malondialdehyde (MDA), the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px),and the expression of antioxidant related genes in HepG2 cells. The results showed that the activities of SOD and GSH-Px were significantly increased (P<0.05), while ROS and MDA were significantly reduced (P<0.05) after the treatment with fermented hemp seed protein powder. The expression levels of NQO1, Nrf2 and HO-1 mRNA in fermented hemp seed protein powder were increased. The results showed that the Nrf2 pathway was activated by fermented hemp seed protein powder to alleviate the oxidative stress damage of HepG2 induced by AAPH.
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