该文研究了南极磷虾油(antarctic krill oil,AKO)对衰老模型小鼠的抗氧化作用。无特定病原体(specific pathogen free,SPF)级癌症研究学会(institute of cancer research,ICR)健康雄性小鼠培养达到8~10月龄构建衰老模型小鼠,将衰老模型小鼠随机分成4组,每组10只,设置南极磷虾油处理低剂量组(L-AKO)0.075 g/kg·BW、中剂量组(M-AKO)0.150 g/kg·BW、高剂量组(H-AKO)0.450 g/kg·BW和阴性对照组,阴性对照组给予等体积的食用植物油,每天灌胃1次,连续灌胃30 d。30 d后,测定过氧化脂质(lipid peroxide,LPO)含量[以丙二醛(malondialdehyde,MDA)含量计]、还原型谷胱甘肽(reduced glutathione,GSH)含量、超氧化物歧化酶(superoxide dismutase,SOD)活力及蛋白质羰基(protein carbonyl,PCO)含量。结果表明,AKO中、高剂量组能分别显著和极显著提高血清SOD活力(P<0.05,P<0.01)和GSH含量(P<0.05,P<0.01),显著降低血液中LPO含量(P<0.05),PCO含量无显著变化(P＞0.05)。研究证明AKO对衰老模型小鼠具有抗氧化作用。
The aim of this study was to explore the antioxidant effect of Antarctic krill oil (AKO) on aging model mice. SPF-ICR healthy male mice aged 8-10 months were randomly divided into 4 groups, low-dose group, medium-dose group, high-dose krill oil group and one negative control group. Ten mice in each group and were given a dose of 0.075 g/kg·BW, 0.150 g/kg·BW, 0.450 g/kg·BW and corn germ oil respectively. The four mice groups were dosed by gavage once a day for 30 days. After 30 days, the contents of lipid peroxide (LPO) (calculated by the content of MDA), reduced glutathione (GSH), superoxide dismutase (SOD) and protein carbonyl (PCO) were determined. The results showed that compared with the control group, the activity of serum SOD and serum level of GSH increased considerably (P<0.05) in the medium dose of the krill oil group, and even significantly (P<0.01) in the high-dose group. The level of LPO in the medium dose group was lower (P<0.05) than the control group. However, there was no statistical difference for the level of PCO between the medium dose group and the control group (P>0.05). These results revealed AKO has an antioxidant effect on aging model mice.
[1] 陈瑾歆, 唐聪明.氧自由基的研究进展[J].海南医学院学报, 2004, 10(3):206-208.
CHEN J X, TANG C M.Research progress of oxygen free radicals[J].Journal of Hainan Medical College, 2004, 10(3):206-208.
[2] NICOL S, FOSTER J.Recent trends in the fishery for Antarctic krill[J].Aquatic Living Resources, 2003, 16(1):42-45.
[3] 孙雷, 周德庆, 盛晓风.南极磷虾营养评价与安全性研究[J].海洋水产研究, 2008, 29(2):57-64.
SUN L, ZHOU D Q, SHENG X F.Nutrition and safety evaluation of Antarctic krill[J].Marine Fisheries Research, 2008, 29(2):57-64.
[4] SAVAGE G P, FOULDS M J.Chemical composition and nutritive value of Antarctic krill (Euphausia superba) and southern blue whiting (Micromesistius australis)[J].New Zealand Journal of Marine and Freshwater Research, 1987, 21(4):599-604.
[5] 施佳慧, 吕桂善, 徐同成, 等.磷虾油的脂肪酸成分及其降血脂功能研究[J].营养学报, 2008, 30(1):115-116.
SHI J H, LYU G S, XU T C, et al.Study on fatty acids components and the hypolipidemic activity of superba oil[J].Acta Nutrimenta Sinica, 2008, 30(1):115-116.
[6] WERNER A, HAVINGA R, KUIPERS F, et al.Treatment of EFA deficiency with dietary triglycerides or phospholipids in a murine model of extrahepatic cholestasis[J].American Journal of Physiology.Gastrointestinal and Liver Physiology, 2004, 286(5):G822-G832.
[7] KIDD P M.Omega-3 DHA and EPA for cognition, behavior, and mood:Clinical findings and structural-functional synergies with cell membrane phospholipids[J].Alternative Medicine Review, 2007, 12(3):207-227.
[8] 刘丽, 刘承初, 赵勇, 等.南极磷虾的营养保健功效以及食用安全性评价[J].食品科学, 2010, 31(17):443-447.
LIU L, LIU C C, ZHAO Y, et al.Recent advances in the healthcare function and food safety of Antarctic krill[J].Food Science, 2010, 31(17):443-447.
[9] 贺瑞坤, 罗海吉.南极磷虾油对人类健康的作用[J].食品研究与开发, 2013, 34(20):130-133.
HE R K, LUO H J.Health benefits of Antarctic krill oil[J].Food Research and Development, 2013, 34(20):130-133.
[10] 王亚恩. 南极磷虾油降血脂、抗氧化力及其改善记忆力功能实验研究[D].青岛:中国海洋大学, 2011.
WANG Y E.Experimental study of the Antarctic krill oil on the serum lipids and antioxidation ability and memory ability[D].Qingdao:Ocean University of China, 2011.
[11] ZWART L L D, MEERMAN J H N, COMMANDEUR J N M, et al.Biomarkers of free radical damage applications in experimental animals and in humans[J].Free Radical Biology & Medicine, 1999, 26(1-2):202-226.
[12] SAIRAM R K, SINGH D V, SRIVASTAVA G C.Changes in activities of antioxidant enzymes in sunflower leaves of different ages[J].Biologia Plantarum, 2003, 47(1):61-66.
[13] AIT Y D, SIHEM M, EMMANUELLE P, et al.Tissue antioxidant status differs in spontaneously hypertensive rats fed fish protein or casein[J].Journal of Nutrition, 2003, 133(2):479-482.
[14] 张玉臣, 杜永丽, 聂翠芳, 等.还原型谷胱甘肽的临床应用进展[J].社区医学杂志, 2005, 3(4):26-27.
ZHANG Y C, DU Y L, NIE C F, et al.Clinical application progress of reduced glutathione sodjum[J].Journal of Community Medicine, 2005, 3(4):26-27.
[15] 文镜, 张春华, 董雨, 等.蛋白质羰基含量与蛋白质氧化损伤[J].食品科学, 2003, 24(10):153-157.
WEN J, ZHANG C H, DONG Y, et al.Review on relationship between protein carbonyl content and protein oxidative injury[J].Food Science, 2003, 24(10):153-157.
[16] 杨艳, 周宇红, 徐海滨.虾青素抗氧化活性机制研究进展[J].国外医学 (卫生学分册), 2008, 35(4):231-234.
YANG Y, ZHOU Y H, XU H B.Research progress on antioxidant mechanism of astaxanthin[J].Foreign Medical Sciences (Section Hygiene), 2008, 35(4):231-234.
[17] JANINA D, SEIICHI M, HARUKA Y, et al.Free radical scavenging and cellular antioxidant properties of astaxanthin[J].International Journal of Molecular Sciences, 2016, 17(1).DOI:10.3390/ijms17010103.
[18] 刘仁海, 黄瑾, 何玲, 等.磷脂酰胆碱抗氧化作用的研究[J].农垦医学, 1995 (4):222-223.
LIU R H, HUANG J, HE L, et al.Studies on antioxidation of phosphatidylcholine[J].Agricultural Reclamation Medicine, 1995 (4):222-223.
[19] 费云芸, 董贵俊.磷脂对小鼠运动耐力及肝脏、骨骼肌组织抗氧化能力的影响研究[J].吉林体育学院学报, 2011, 27(5):77-78.
FEI Y Y, DONG G J.Effects of phospholipids on delaying fatigue and anti-oxidation in mice′s liver and skeletal muscle[J].Journal of Jilin Institute of Physical Education, 2011, 27(5):77-78.
[20] 胡滨, 陈一资, 王雪铭, 等.红花籽油的抗氧化功能研究[J].中国粮油学报, 2016, 31(6):86-91.
HU B, CHEN Y Z, WANG X M, et al.The antioxidant activity of safflower seed oil[J].Journal of the Chinese Cereals and Oils Association, 2016, 31(6):86-91.
