食品与发酵工业 >

2021 , Vol. 47 >Issue 16: 173 - 180

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027381

生产与科研应用

热处理对负载叶黄素的罗非鱼分离蛋白乳液稳定性和体外消化的影响

  • 陈艾霖 ,
  • 洪鹏志 ,
  • 宋春勇 ,
  • 冯瑞 ,
  • 李婷 ,
  • 周春霞 ,
  • 林琼妮
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  • 1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东 湛江,524088)
    2(南方海洋科学与工程广东省实验室(湛江),广东 湛江,524088)
硕士研究生(周春霞副教授为通讯作者,E-mail:zhoucx@gdou.edu.cn)

收稿日期: 2021-03-15

  修回日期: 2021-04-17

  网络出版日期: 2021-09-10

基金资助

广东省科技计划项目(2017A020208067);广东省现代农业产业技术体系创新团队建设项目(2019KJ150)

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Effect of heat treatment on stability and in vitro digestion of lutein loaded tilapia protein isolate

  • CHEN Ailin ,
  • HONG Pengzhi ,
  • SONG Chunyong ,
  • FENG Rui ,
  • LI Ting ,
  • ZHOU Chunxia ,
  • LIN Qiongni
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  • 1(College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Zhanjiang 524088, China)
    2(Southern Marine Science and Engineering Guangdong Laboratory (Zhanjiang), Zhanjiang 524088, China)

Received date: 2021-03-15

  Revised date: 2021-04-17

  Online published: 2021-09-10

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摘要

叶黄素是一种具有多种生物活性的类胡萝卜素,因高疏水性和低稳定性,其应用受到限制。为提升叶黄素的稳定性和生物利用率,以罗非鱼分离蛋白(tilapia protein isolate,TPI)作为乳化剂,高压均质制备负载叶黄素(200 μg/mL)的TPI乳液,探讨热处理(60、70、80、90 ℃,30 min)对乳液的稳定性和体外消化的影响。结果表明,热处理后,叶黄素乳液的粒径减小(P<0.05),电位绝对值增大(P<0.05),4 ℃下贮藏28 d无分层现象,且热处理温度低于80 ℃不会造成叶黄素降解。热处理能增加液滴的表面静电荷和乳液黏度,有利于抵抗液滴之间的聚集,乳液稳定性增加。其中,70 ℃加热30 min后叶黄素乳液的稳定性最好,乳液粒径减小至(490.33±8.42) nm,游离脂肪酸释放量达(94.22±2.67)%,叶黄素生物利用率为(35.69±2.06)%。TPI乳液能够作为疏水活性物质的载体,且热处理能明显提高乳液的稳定性和叶黄素的生物利用率,为叶黄素的应用提供新思路。

关键词： 罗非鱼分离蛋白; 叶黄素; 乳液; 稳定性; 体外消化

本文引用格式

陈艾霖 , 洪鹏志 , 宋春勇 , 冯瑞 , 李婷 , 周春霞 , 林琼妮 . 热处理对负载叶黄素的罗非鱼分离蛋白乳液稳定性和体外消化的影响[J]. 食品与发酵工业, 2021 , 47(16) : 173 -180 . DOI: 10.13995/j.cnki.11-1802/ts.027381

Abstract

Lutein is a kind of carotenoid with many biological activities. Its application is limited due to its high hydrophobicity and low stability. In order to improve the stability and bioavailability of lutein, tilapia protein isolate (TPI) emulsion loading with lutein (200 μg/mL) was prepared by high pressure homogenization using tilapia protein isolate (TPI) as an emulsifying agent. The effects of heat treatment (60, 70, 80, 90 ℃, 30 min) on the stability and digestion of the emulsion were investigated. The results showed that with heat treatment, the particle size of lutein emulsion decreased (P<0.05), however, the absolute potential increased (P<0.05). And there was no delamination at 4 ℃ for 28 d. Moreover, the degradation of lutein was not caused when the heat treatment temperature was lower than 80 ℃. Besides, the heat treatment could increase the surface static charge of the droplet and the viscosity of the emulsion, which was beneficial to resist the aggregation of the droplet and increase the stability of the emulsion. After heat treatment at 70 ℃ for 30 min, lutein emulsion had the best stability, the particle size of the emulsion was reduced to (490.33 ± 8.42) nm. And the release amount of free fatty acids was (94.22±2.67)% and the bioavailability of lutein was (35.69±2.06)% respectively. TPI emulsion can be used as a carrier of hydrophobic active substances. And heat treatment can significantly improve the stability of the emulsion and the bioavailability of lutein, which provides new insights for the application of lutein.

Key words： tilapia protein isolate; lutein; emulsion; stability; in vitro digestion

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