综述与专题评论

微生物法从头合成2-苯乙醇的研究进展

  • 朱灵桓 ,
  • 徐沙 ,
  • 李由然 ,
  • 张梁 ,
  • 石贵阳
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  • 1(江南大学 生物工程学院,江苏 无锡,214122)
    2(粮食发酵工艺与技术国家工程实验室,江苏 无锡,214122)
    3(河北科技大学 食品与生物学院,河北 石家庄,050018)
博士,讲师(石贵阳教授为通讯作者,E-mail:gyshi@jiangnan.edu.cn)

收稿日期: 2021-04-26

  修回日期: 2021-04-30

  网络出版日期: 2021-09-10

基金资助

国家自然科学基金项目(31571817)

Recent advances on de novo biosynthesis of 2-phenylethanol

  • ZHU Linghuan ,
  • XU Sha ,
  • LI Youran ,
  • ZHANG Liang ,
  • SHI Guiyang
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  • 1(School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2(National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi 214122, China)
    3(College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050018, China)

Received date: 2021-04-26

  Revised date: 2021-04-30

  Online published: 2021-09-10

摘要

2-苯乙醇具有令人愉悦的玫瑰花香气和良好的理化性质,是一种被广泛应用于食品、医药与日用化工行业的芳香醇。由于市场前景良好,高品质2-苯乙醇产品的需求量逐年上升,绿色高效的微生物发酵制备策略成为近年来的研究热点。该文对微生物中2-苯乙醇的从头合成途径进行了综述,分析了相关的调控机理,并对多种代谢策略进行了评价和展望。

本文引用格式

朱灵桓 , 徐沙 , 李由然 , 张梁 , 石贵阳 . 微生物法从头合成2-苯乙醇的研究进展[J]. 食品与发酵工业, 2021 , 47(16) : 271 -277 . DOI: 10.13995/j.cnki.11-1802/ts.027827

Abstract

2-Phenylethanol is widely used in cosmetics, food and medicine industries owing to its favorable flavors and superior properties. However, the output of pollution-free and high-quality aromatic alcohols remains limited, which make the biosynthesis of 2-phenylethanol gradually become a research hotspot. In this study, the de novo synthetic pathway of 2-phenylethanol is reviewed, relative regulatory mechanism is analyzed, and various metabolic strategies are evaluated and prospected.

参考文献

[1] KROMER J O, BERNAL D, AVERESCH N J, et al.Production of aromatics in Saccharomyces cerevisiae-a feasibility study[J].J Biotechnol, 2013,163(2):184-193.
[2] ETSCHMANN M M W, BLUEMKE W, SELL D, et al.Biotechnological production of 2-phenylethanol[J].Applied Microbiolgy and Biotechnology, 2002,59(1):1-8.
[3] HUA D L, XU P.Recent advances in biotechnological production of 2-phenylethanol[J].Biotechnol Adv, 2011,29(6):654-660.
[4] SCHRADER J, ETSCHMANN M M W, SELL D, et al.Applied biocatalysis for the synthesis of natural flavour compounds current industrial processes and future prospects[J].Biotechnology Letters, 2004,26(6):463-472.
[5] WANG Y Q, ZHANG H, LU X Y, et al.Advances in 2-phenylethanol production from engineered microorganisms[J].Biotechnology Advances, 2019,37(3):403-409.
[6] GU Y, MA J B, ZHU Y L, et al.Engineering Yarrowia lipolytica as a chassis for de novo synthesis of five aromatic-derived natural products and chemicals[J].ACS Synthetic Biology, 2020,9(8):2 096-2 106.
[7] 牛明福,李亚恒,陈金帅,等.马克斯克鲁维酵母生物转化2-PE工艺优化及耐高温特性分析[J].食品与发酵工业, 2018,44(2):15-20.
NIU M F,LI Y H,CHEN J S, et al.Optimization and characterization of 2-phenylethanol bioconversion by thermotolerant yeast Kluyveromyces marxianus[J].Food and Fermentation Industries, 2018, 44(2):15-20.
[8] RAJKUMAR A S, MORRISSEY J P.Rational engineering of Kluyveromyces marxianus to create a chassis for the production of aromatic products[J].Microbial Cell Factories, 2020,19(1):207.
[9] DAI J, LI K, SONG N, et al.Zygosaccharomyces rouxii, an aromatic yeast isolated from chili sauce, is able to biosynthesize 2-phenylethanol via the shikimate or Ehrlich pathways[J].Frontiers in Microbiology, 2020,11:597 454.
[10] YAN W, ZHANG X Y, QIAN X J, et al.Comprehensive investigations of 2-phenylethanol production by high 2-phenylethanol tolerating Meyerozyma sp.strain YLG18[J].Enzyme and Microbial Technology, 2020,140:109 629.
[11] JIN D F, GU B T, XIONG D W, et al.A Transcriptomic analysis of Saccharomyces cerevisiae under the stress of 2-phenylethanol[J].Curr Microbiol, 2018,75(8):1 068-1 076.
[12] HASSING E J, DE GROOT P A, MARQUENIE V R, et al.Connecting central carbon and aromatic amino acid metabolisms to improve de novo 2-phenylethanol production in Saccharomyces cerevisiae[J].Metabolic Engineering, 2019,56:165-180.
[13] HELMSTAEDT K, STRITTMATTER A, LIPSCOMB W N,et al.Evolution of 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase-encoding genes in the yeast Saccharomyces cerevisiae[J].PNAS, 2005,102(28):9 784–9 789.
[14] RODRIGUEZ A, KILDEGAARD K R, LI M J, et al.Establishment of a yeast platform strain for production of p-coumaric acid through metabolic engineering of aromatic amino acid biosynthesis[J].Metabolic Engineering, 2015,31:181-188.
[15] LIU Q L, YU T, LI X W, et al.Rewiring carbon metabolism in yeast for high level production of aromatic chemicals[J].Nature Communications, 2019,10(1):4 976.
[16] KIM T Y, LEE S W, OH M K.Biosynthesis of 2-phenylethanol from glucose with genetically engineered Kluyveromyces marxianus[J].Enzyme and Microbial Technology, 2014,61-62:44-47.
[17] GUO D Y, ZHANG L H, KONG S J, et al.Metabolic engineering of Escherichia coli for production of 2-phenylethanol and 2-phenylethyl acetate from glucose[J].Journal of Agricultural and Food Chemistry, 2018,66(23):5 886-5 891.
[18] MACHAS M S, MCKENNA R, NIELSEN D R.Expanding upon styrene biosynthesis to engineer a novel route to 2-phenylethanol[J].Biotechnol Journal, 2017,12(10):1 700 310.
[19] KONG S J, PAN H, LIU X Y, et al.De novo biosynthesis of 2-phenylethanol in engineered Pichia pastoris[J].Enzyme and Microbial Technology, 2020,133:109 459.
[20] SUÁSTEGUI M, GUO W H, FENG X Y, et al.Investigating strain dependency in the production of aromatic compounds in Saccharomyces cerevisiae[J].Biotechnology and Bioengineering, 2016,113(12):2 676-2 685.
[21] GOLD N D, GOWEN C M, LUSSIER F X, et al.Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics[J].Microbial Cell Factories, 2015,14:73.
[22] GUO W, HUANG Q L, LIU H, et al.Rational engineering of chorismate-related pathways in Saccharomyces cerevisiae for improving tyrosol production[J].Frontiers in Bioengineering and Biotechnology, 2019,7:152.
[23] GUO W, HUANG Q L, FENG Y H, et al.Rewiring central carbon metabolism for tyrosol and salidroside production in Saccharomyces cerevisiae[J].Biotechnology and Bioengineering, 2020,117(8):2 410-2 419.
[24] CURRAN K A, LEAVITT J M, KARIM A S, et al.Metabolic engineering of muconic acid production in Saccharomyces cerevisiae[J].Metabolic Engineering, 2013,15:55-66.
[25] ZHU L H, WANG J H, XU S, et al.Improved aromatic alcohol production by strengthening the shikimate pathway in Saccharomyces cerevisiae[J].Process Biochemistry, 2021,103:18-30.
[26] BERGMAN A, HELLGREN J, MORITZ T, et al.Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae[J].Microbial Cell Factories, 2019,18(1):25.
[27] SUÁSTEGUI M, YU N C, CHOWDHURY A, et al.Multilevel engineering of the upstream module of aromatic amino acid biosynthesis in Saccharomyces cerevisiae for high production of polymer and drug precursors[J].Metabolic Engineering, 2017,42:134-144.
[28] NOCON J, STEIGER M, MAIRINGER T, et al.Increasing pentose phosphate pathway flux enhances recombinant protein production in Pichia pastoris[J].Applied Microbiology and Biotechnology, 2016,100(13):5 955-5 963.
[29] LYU X M, NG K R, MARK R, et al.Comparative metabolic profiling of engineered Saccharomyces cerevisiae with enhanced flavonoids production[J].Journal of Functional Foods, 2018,44:274-282.
[30] LUTTIK M A H, VURALHAN Z, SUIR E, et al.Alleviation of feedback inhibition in Saccharomyces cerevisiae aromatic amino acid biosynthesis:Quantification of metabolic impact[J].Metabolic Engineering, 2008,10(3):141-153.
[31] LI M W, LANG X Y, MORAN CABRERA M, et al.CRISPR-mediated multigene integration enables Shikimate pathway refactoring for enhanced 2-phenylethanol biosynthesis in Kluyveromyces marxianus[J/OL].Biotechnology for Biofuels, 2021,14(1).DOI:10.1186/S13068-020-01852-3.
[32] LIU S P, XIAO M R, ZHANG L, et al.Production of L-phenylalanine from glucose by metabolic engineering of wild type Escherichia coli W3110[J].Process Biochem, 2013,48(3):413-419.
[33] ZHANG C Z, KANG Z, ZHANG J L, et al.Construction and application of novel feedback-resistant 3-deoxy-d-arabino-heptulosonate-7-phosphate synthases by engineering the N-terminal domain for L-phenylalanine synthesis[J].FEMS Microbiology Letters, 2014,353(1):11-18.
[34] REIFENRATH M, BAUER M, OREB M, et al.Bacterial bifunctional chorismate mutase-prephenate dehydratase PheA increases flux into the yeast phenylalanine pathway and improves mandelic acid production[J].Metabolic Engineering Communications, 2018,7:e00 079.
[35] LIU S P, LIU R X, XIAO M R, et al.A systems level engineered E.coli capable of efficiently producing L-phenylalanine[J].Process Biochemistry, 2014,49(5):751-757.
[36] ZHANG H B, CAO M L, JIANG X L, et al.De novo synthesis of 2-phenylethanol by Enterobacter sp.CGMCC 5087[J].BMC Biotechnology, 2014,14(1):1-7.
[37] LIU S P, YANG Q L, MAO J Q, et al.Feedback inhibition of the prephenate dehydratase from Saccharomyces cerevisiae and its mutation in Huangjiu (Chinese rice wine) yeast[J].LWT, 2020,133:110 040.
[38] ROMAGNOLI G, KNIJNENBURG T A, LITI G, et al.Deletion of the Saccharomyces cerevisiae ARO8 gene, encoding an aromatic amino acid transaminase, enhances phenylethanol production from glucose[J].Yeast, 2015,32(1):29-45.
[39] WANG Y Q, ZHANG Z Y, LU X Y, et al.Genetic engineering of an industrial yeast Candida glycerinogenes for efficient production of 2-phenylethanol[J].Applied Microbiology and Biotechnology, 2020,104(24):10 481-10 491.
[40] VURALHAN Z, LUTTIK M A H, TAI S L, et al.Physiological characterization of the ARO10-dependent, broad-substrate-specificity 2-oxo acid decarboxylase activity of Saccharomyces cerevisiae[J].Appl Biochem Biotechnol, 2005,71(6):3 276-3 284.
[41] VURALHAN Z, MORAIS M A, TAI S L, et al.Identification and characterization of phenylpyruvate decarboxylase genes in Saccharomyces cerevisiae[J].Appl Biochem Biotechnol, 2003,69(8):4 534-4 541.
[42] KNEEN M M, STAN R, YEP A, et al.Characterization of a thiamin diphosphate-dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae[J].FEBS J, 2011,278(11):1 842-1 853.
[43] STRIBNY J, ROMAGNOLI G, PÉREZ-TORRADO R, et al.Characterisation of the broad substrate specificity 2-keto acid decarboxylase Aro10p of Saccharomyces kudriavzevii and its implication in aroma development[J].Microbial Cell Factories, 2016,15(1):1-12.
[44] BOLAT I, ROMAGNOLI G, ZHU F B, et al.Functional analysis and transcriptional regulation of two orthologs of ARO10, encoding broad-substrate-specificity 2-oxo-acid decarboxylases, in the brewing yeast Saccharomyces pastorianus CBS1483[J].FEMS Yeast Research, 2013,13(6):505-517.
[45] KANG Z, ZHANG C Z, DU G C, et al.Metabolic engineering of Escherichia coli for production of 2-phenylethanol from renewable glucose[J].Applied Biochemistry and Biotechnology, 2014,172(4):2 012-2 021.
[46] GUO D Y, ZHANG L H, PAN H, et al.Metabolic engineering of Escherichia coli for production of 2-phenylethylacetate from L-phenylalanine[J].MicrobiologyOpen, 2017,6(4):1-5.
[47] MO Q W, CHEN H Y, FAN C, et al.Utilization of a styrene-derived pathway for 2-phenylethanol production in budding yeast[J].Applied Microbiology and Biotechnology, 2021,105(6):2 333-2 340.
[48] YANG D D, BILLERBECK G, ZHANG J J.Deciphering the origin, evolution, and physiological function of the subtelomeric aryl-alcohol dehydrogenase gene family in the yeast Saccharomyces cerevisiae[J].Applied and Environmental Microbiology, 2018,84(1):1-16.
[49] LIMA L A, VENTORIM R Z, BIANCHINI I A, et al.Obtainment, selection and characterization of a mutant strain of Kluyveromyces marxianus that displays improved production of 2-phenylethanol and enhanced DAHP synthase activity[J].Journal of Applied Microbiology, 2021,130(3):878-890.
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