食品与发酵工业 >

2021 , Vol. 47 >Issue 17: 69 - 75

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026607

研究报告

脱氢乙酸钠对杀鲑气单胞菌群体感应现象的抑制

  • 王俊淇 ,
  • 李婷婷 ,
  • 刘佳宜 ,
  • 谢晶 ,
  • 林洪 ,
  • 励建荣
展开
  • 1(渤海大学 食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁 锦州,121013)
    2(大连民族大学 生命科学学院,辽宁 大连,116600)
    3(上海海洋大学 食品学院,上海,201306)
    4(中国海洋大学 食品学院,山东 青岛,266100)
硕士研究生(李婷婷教授和励建荣教授为共同通讯作者,E-mail: tingting780612@163.com;lijr6491@163.com)

收稿日期: 2020-10-30

  修回日期: 2021-02-25

  网络出版日期: 2021-09-27

基金资助

“十三五”国家重点研发计划蓝色粮仓科技创新重点专项(2019YFD0901702 )

收起

Inhibition of sodium dehydroacetate on quorum sensing of Aeromonas salmonicida

  • WANG Junqi ,
  • LI Tingting ,
  • LIU Jiayi ,
  • XIE Jing ,
  • LIN Hong ,
  • LI Jianrong
Expand
  • 1(National & Local Joint Engineering Research of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products,College of Food Science and Engineering,Bohai University,Jinzhou 121013,China)
    2(College of Life Science,Dalian Nationalities University,Dalian 116600,China)
    3(College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China)
    4(College of Food Science and Technology,Ocean University of China,Qingdao 266100,China)

Received date: 2020-10-30

  Revised date: 2021-02-25

  Online published: 2021-09-27

Fold

摘要

利用报告菌株紫色杆菌(Chromobacterium violaceum 026,CV026)来研究脱氢乙酸钠的群体感应抑制作用,并进一步研究其对杀鲑气单胞菌的群体感应作用效果。结果表明:当脱氢乙酸钠质量浓度为2 mg/mL时,CV026的生长不会受到抑制,且在亚抑菌浓度条件下,脱氢乙酸钠可显著降低报告菌株CV026产生紫色杆菌素的能力;当脱氢乙酸钠质量浓度为1.6 mg/mL时,紫色菌素的产量下降63.39%。通过酶标法可以明显看出脱氢乙酸钠使杀鲑气单胞菌生物被膜的形成量降低,进一步利用扫描电镜观察,生物被膜相对形成率仅为31.78%;通过其他的群体感应现象如:群集和泳动能力分别下降了82.76%和73.41%,胞外蛋白酶活性下降了14.56%,进一步验证了脱氢乙酸钠的群体感应抑制活性,且呈现浓度依赖性。同时,通过气相色谱-质谱联用的检测证实了脱氢乙酸钠对杀鲑气单胞菌信号分子的产生具有抑制作用。由此可知,脱氢乙酸钠具有群体感应抑制性,能够作为新型的群体感应抑制剂。

关键词： 脱氢乙酸钠; 群体感应; 群体感应抑制剂; 抑制效果; 杀鲑气单胞菌

本文引用格式

王俊淇 , 李婷婷 , 刘佳宜 , 谢晶 , 林洪 , 励建荣 . 脱氢乙酸钠对杀鲑气单胞菌群体感应现象的抑制[J]. 食品与发酵工业, 2021 , 47(17) : 69 -75 . DOI: 10.13995/j.cnki.11-1802/ts.026607

Abstract

Chromobacterium violaceum 026 (CV026) was used as a report strain to evaluated the quorum sensing inhibition of sodium dehydroacetate on Aeromonas salmonicida. The results showed that the growth of CV026 was uninhibited with 2 mg/mL sodium dehydroacetate. The ability of CV026 to produce violacein was reduced with sodium dehydroacetate on subinhibitory. The yield of violacein was decreased by 63.39% with sodium dehydroacetate of 1.6 mg/mL. The enzyme labeling results showed that sodium dehydroacetate significantly reduced the formation of A. salmonicida biofilm, and the relative formation rate of biofilm was only 31.78%. In addition, the clustering and swimming ability decreased by 82.76% and 73.41%, respectively. The activity of extracellular protease was decreased by 14.56%. At the same time, the inhibition of sodium dehydroacetate on the production of signal molecules was confirmed by GC-MS. Therefore, sodium dehydroacetate has quorum inhibition and can be used as a new quorum induction inhibitor.

Key words： sodium dehydroacetate; quorum sensing; quorum sensing inhibition; inhibitory effect; Aeromonas salmonicida

参考文献

[1] DALGAARD P.Qualitative and quantitative characterization of spoilage bacteria from packed fish[J].International Journal of Food Microbiology,1995,26(3):319-333.
[2] 励建荣, 李婷婷,王当丰.微生物群体感应系统及其在现代食品工业中应用的研究进展[J].食品科学技术学报,2020,38(1):1-11.
LI J R,LI T T,WANG D F.Research progress on microbial quorum sensing system and its application in modern food industry[J].Journal of Food Science and Technology,2020,38(1):1-11.
[3] GANESH P S,RAI V R.Evaluation of anti-bacterial and anti-quorum sensing potential of essential oils extracted by supercritical CO2 method against Pseudomonas aeruginosa[J].Journal of Essential Oil Bearing Plants,2015,18(2):264-275.
[4] 牛慧超. 1,10-癸二醇对杀鲑气单胞菌群体感应抑制作用及对大黄鱼保鲜效果研究[D].锦州:渤海大学,2020.
NIU H C.Study on the inhibitory effect of 1,10-decanediol on quroum sensing of Aeromonas salmon and its preservation effect on Larimichthys crocea[D].Jinzhou:Bohai University,2020.
[5] 张宁. 基于群体感应途径探讨茶多酚对肺炎克雷伯菌毒力及耐药性的作用[D].南昌:南昌大学,2020.
ZHANG N.Effects of tea polyphenols on virulence and drug resistance of Klebsiella pneumoniae based on quorum sensing[D].Nanchang:Nanchang University,2020.
[6] 丁婷, 李勇.米曲霉来源的环二肽对荧光假单胞菌群体感应的抑制及其机制[J].生物加工过程,2020,18(2):234-244.
DING T,LI Y.Inhibition on quorum sensing of Pseudomonas fluorescens by cyclic dipeptide isolated from Aspergillus oryzae and its mechanism[J].Chinese Journal of Bioprocess Engineering,2020,18(2):234-244.
[7] ZHENG R H,XU X R,XING J L,et al.Quality evaluation and characterization of specific spoilage organisms of Spanish mackerel by high-throughput sequencing during 0 ℃ cold chain logistics[J].Foods,2020,9(3):312.
[8] 于红雷, 韩云艳,曾名湧,等.凡纳滨对虾源气单胞菌的分离鉴定及群体感应[J].生物加工过程,2019,17(3):324-328.
YU H L,HAN Y Y,ZENG M Y,et al.Identification and quorum sensing of Aeromonas from Litopenaeus vannamei[J].Chinese Journal of Bioprocess Engineering,2019,17(3):324-328.
[9] GARDE C,BJARNSHOLT T,GIVSKOV M,et al.Quorum sensing regulation in Aeromonas hydrophila[J].Journal of Molecular Biology,2010,396(4):849-857.
[10] KHAJANCHI B K,KOZLOVA E V,SHA J,et al.The two-component QseBC signalling system regulates in vitro and in vivo virulence of Aeromonas hydrophila[J].Microbiology,2012,158(1):259-271.
[11] 雷美华.杀鲑气单胞菌对鱼类致病性的研究[J].海洋与渔业,2020(3):68-69.
LEI M H.Studies on the pathogenicity of salmon killing Aeromonas on fish[J].Ocean and Fishery,2020(3):68-69.
[12] 葛阳杨, 朱军莉,刘丽,等.大黄鱼源气单胞菌的分离鉴定及致腐表型[J].水产学报,2017,41(6):861-869.
GE Y Y,ZHU J L,LIU L,et al.Spoilage phenotypic characterization and identification of Aeromonas isolated from spoiled Larimichthys crocea[J].Journal of Fisheries of China,2017,41(6):861-869.
[13] 叶银枝. 脱氢醋酸钠在食品中的应用[J].中国食品添加剂,2002(3):64-66.
YE Y Z.Use of sodium dehydroacetate in food[J].China Food Additives,2002(3):64-66.
[14] 黎婉园, 姚朔影,夏枫耿,等.脱氢醋酸钠及其抗菌性试验[J].中国食品添加剂,2004(2):41-44.
LI W Y,YAO S Y,XIA F G,et al.Studied on antibacterial properties of disodium dehydroacetate[J].China Food Additives,2004(2):41-44.
[15] 吴雅萍,江晓佟,张帅,等.乳链球菌素最小抑菌浓度(MIC)测定及理化性质分析[J].中国食品添加剂,2020,31(5):119-123.
WU Y P,JIANG X T,ZHANG S,et al.Nisin minimum inhibitory concentration (MIC) determination and its physicochemical property[J].China Food Additives,2020,31(5):119-123.
[16] 孔凡栋, 周丽曼,马青云,等.革兰氏阴性细菌LuxR/Ⅰ型群体感应抑制剂研究进展[J].现代生物医学进展,2017,17(30):5 984-5 988.
KONG F D,ZHOU L M,MA Q Y,et al.Research advances in the LuxR/I type quorum sensing inhibitors of gram-negative bacteria[J].Progress in Modern Biomedicine,2017,17(30):5 984-5 988.
[17] 赵龙华,杨维青.细菌群集运动与生物被膜和耐药性的关系[J].国际检验医学杂志,2011,32(17):1 986-1 988.
ZHAO L H,YANG W Q.The relationship between bacterial swarm movement and biofilm and drug resistance[J].International Journal of Laboratory Medicine,2011,32(17):1 986-1 988.
[18] LEE S O,KATO J,TAKIGUCHI N,et al.Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp.strain A28[J].Applied and Environmental Microbiology,2000,66(10):4 334-4 339.
[19] WEI L N,SHI C Z,LUO C X,et al.Phloretin inhibits biofilm formation by affecting quorum sensing under different temperature[J].LWT,2020,131.DOI:10.1016/j.lwt.2020.109668.
[20] 赵朵, 肖娜,裴曼君,等.金黄色葡萄球菌生物被膜形成及检测方法研究[J].教育教学论坛,2020(47):388-390.
ZHAO D,XIAO N,PEI M J,et al.Research on the formation and detection methods of Staphylococcus aureus biofilm[J].Education Teaching Forum,2020(47):388-390.
[21] 孙晓佳, 李婷婷,赫彬彬,等.富马酸钠对荧光假单胞菌群体感应现象及其腐败活性的抑制作用[J].食品科学,2019,40(11):7-13.
SUN X J,LI T T,HE B B,et al.Inhibitory effect of sodium fumarate on quorum sensing and spoilage capacity of Pseudomonas fluorescens[J].Food Science,2019,40(11):7-13.
[22] 渠宏雁, 李学鹏,孟良玉,等.抑制剂对副溶血性弧菌运动性的影响[J].食品与生物技术学报,2014,33(5):480-485.
QU H Y,LI X P,MENG L Y,et al.Effect of inhibitors on the motilities of Vibrio parahaemolyticus[J].Journal of Food Science and Biotechnology,2014,33(5):480-485.
[23] 周围. 粘质沙雷氏菌次生代谢产物灵菌红素的生理与生物学活性的研究[D].重庆:西南大学,2014.
ZHOU W.The physiological and biological activities of prodigiosin,the secondary metabolites of Serratia marcescens[D].Chongqing:Southwest University,2014.
[24] 梅永超, 李婷婷,刘楠,等.绿薄荷精油对温和气单胞菌群体感应现象及其腐败特性的抑制作用[J].食品科学,2018,39(15):17-23.
MEI Y C,LI T T,LIU N,et al.Effect of spearmint oil on quorum sensing and spoilage characteristics of Aeromonas sobria[J].Food Science,2018,39(15):17-23.
[25] DONLAN R M,COSTERTON J W.Biofilms:Survival mechanisms of clinically relevant microorganisms[J].Clinical Microbiology Reviews,2002,15(2):167-193.
[26] ZHANG K J,YANG X Y,YANG J L,et al.Alcohol dehydrogenase modulates quorum sensing in biofilm formations of Acinetobacter baumannii[J].Microbial Pathogenesis,2020,148.DOI:10.1016/j.micpath.2020.104451.
[27] RANI S,KUMAR A,MALIK A K,et al.Occurrence of N-acyl homoserine lactones in extracts of bacterial strain of Pseudomonas aeruginosa and in sputum sample evaluated by gas chromatography-mass spectrometry[J].American Journal of Analytical Chemistry,2011,2(2):294-302.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司