食品与发酵工业 >

2021 , Vol. 47 >Issue 17: 126 - 132

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027283

研究报告

虾肽对过氧化氢诱导RAW 264.7细胞氧化损伤的保护作用

  • 邵婉 ,
  • 王芮 ,
  • 周宇芳 ,
  • 相兴伟 ,
  • 孙继鹏 ,
  • 王家星 ,
  • 廖妙飞 ,
  • 邓尚贵 ,
  • 郑斌
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  • 1(浙江海洋大学 食品与医药学院,浙江 舟山,316021)
    2(浙江省海洋开发研究院,浙江 舟山,316021)
    3(浙江工业大学 食品科学与工程学院,浙江 杭州,310014)
硕士研究生(邓尚贵教授和郑斌教授为共同通讯作者,E-mail:dengshanggui@163.com;6369958@163.com)

收稿日期: 2021-03-11

  修回日期: 2021-04-01

  网络出版日期: 2021-09-27

基金资助

浙江省重点研发计划项目(2019C02076);舟山市“五大会战”科技项目(2017C12030);浙江省基本科研业务费(2019 J00044)

收起

Protective effects of shrimp peptides on hydrogen peroxide-induced oxidative damage in RAW264.7 Cells

  • SHAO Wan ,
  • WANG Rui ,
  • ZHOU Yufang ,
  • XIANG Xingwei ,
  • SUN Jipeng ,
  • WANG Jiaxing ,
  • LIAO Miaofei ,
  • DENG Shanggui ,
  • ZHENG Bin
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  • 1(College of Food and Pharmacy,Zhejiang Ocean University,Zhoushan 316021,China)
    2(Zhejiang Marine Development Institute,Zhoushan 316021,China)
    3(College of Food Science and Technology,Zhejiang University of Technology,Hangzhou 310014,China)

Received date: 2021-03-11

  Revised date: 2021-04-01

  Online published: 2021-09-27

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摘要

为研究虾肽对H2O2诱导的RAW 264.7细胞氧化损伤的保护作用,利用CCK-8法检测不同剂量虾肽对RAW 264.7细胞的存活率的影响,通过酶联免疫试剂盒和荧光实时定量PCR检测细胞因子和mRNA 的表达水平;Western Blotting检测细胞中核因子E2相关因子2(nuclear factor E2-related factor 2,Nrf2)、Kelch样环氧氯丙烷相关蛋白1(Kelch like-ECH-associated protein 1,Keap1)蛋白含量的变化。结果表明,提前给予虾肽干预的细胞的存活率明显增加,虾肽能提高细胞内超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化酶和过氧化氢酶的活力,而且细胞内SOD 1、谷胱甘肽过氧化物酶1、Nrf2、醌氧化还原酶1、血红素加氧酶-1 mRNA的表达量显著升高(P<0.05)。此外,细胞内Keap1蛋白相对表达量显著降低,而Nrf2蛋白相对表达量显著升高(P<0.05),说明提前给予虾肽干预对H2O2诱导RAW 264.7细胞氧化应激具有预防保护作用,其作用机制与激活Nrf2/Keap1通路有关。

关键词： 虾肽; 过氧化氢; RAW 264.7细胞; 氧化损伤; 保护

本文引用格式

邵婉 , 王芮 , 周宇芳 , 相兴伟 , 孙继鹏 , 王家星 , 廖妙飞 , 邓尚贵 , 郑斌 . 虾肽对过氧化氢诱导RAW 264.7细胞氧化损伤的保护作用[J]. 食品与发酵工业, 2021 , 47(17) : 126 -132 . DOI: 10.13995/j.cnki.11-1802/ts.027283

Abstract

The aim of the study was to investigate the protective effects of shrimp peptides against hydrogen peroxide (H2O2) induced oxidative damage in RAW264.7 cells by CCK-8 assay,enzyme linked immunosorbent assay (ELISA),realtime-PCR and Western Blotting.The results from CCK-8 assay showed that shrimp peptides could significantly increase viability of cells compared with RAW 264.7 cells exposed to H2O2(P<0.05).Moreover,shrimp peptides significantly increase the levels of superoxide dismutase (SOD),glutathione reductase (GSH-Px) and catalase (CAT) in RAW264.7 cells.RT-PCR showed that shrimp peptides could increase the gene expression levels of SOD1,GPX1,Nrf2,NQO1 and HO-1(P<0.05).In addition,shrimp peptides could also observably increase the protein expression level of Nrf2 and decrease the protein expression level of Keap1 with Western Blotting.Collectively,this study suggested that shrimp peptides could effectively protect RAW264.7 cells against H2O2-induced oxidative damage.This protection mechanism may be related to activation of the Nrf2/Keap1 signaling pathway.

Key words： shrimp peptides; hydrogen peroxide; RAW264.7 cells; oxidative damage; protect

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