食品与发酵工业 >

2021 , Vol. 47 >Issue 19: 35 - 42

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026850

研究报告

乳酸片球菌胞外多糖的分离纯化、结构分析及抗氧化活性研究

  • 李尧 ,
  • 卢承蓉 ,
  • 刘丹 ,
  • 韩翔鹏 ,
  • 钟青萍
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  • (华南农业大学 食品学院,广东 广州,510642)
硕士研究生(钟青萍教授为通讯作者,E-mail: zhongqp@scau.edu.cn)

收稿日期: 2021-01-30

  修回日期: 2021-03-09

  网络出版日期: 2021-11-04

基金资助

国家自然科学基金项目(31972046);广东省重点领域研发计划(广东省科技创新战略专项资金项目)(2018B020206001)

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Structure and antioxidant activity of Pediococcus lactis extracellular polysaccharide

  • LI Yao ,
  • LU Chengrong ,
  • LIU Dan ,
  • HAN Xiangpeng ,
  • ZHONG Qingping
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  • (College of Food Science,South China Agricultural University,Guangzhou 510642,China)

Received date: 2021-01-30

  Revised date: 2021-03-09

  Online published: 2021-11-04

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摘要

对1株高产胞外多糖(extracellular polysaccharide,EPS)的乳酸片球菌(Pediococcus lactis C6)的胞外多糖进行分离纯化,采用阴离子交换层析、分子筛层析得到3个纯化组分EPS1、EPS2和EPS3。此3种组分在体外具有一定的抗氧化能力,对DPPH自由基、ABTS阳离子自由基、·OH、·O-2等均有清除作用,其中EPS3、EPS2的抗氧化能力比EPS1强。采用凝胶渗透色谱测定EPS2、EPS3分子质量,分别为1.90×104、2.53×104 Da。采用离子色谱分析胞外多糖的单糖组成,EPS2由木糖、盐酸氨基葡萄糖、半乳糖、葡萄糖组成,摩尔比为0.912∶0.016∶0.022∶0.051;EPS3则由盐酸氨基葡萄糖、半乳糖、葡萄糖、木糖、半乳糖醛酸、葡萄糖醛酸组成,摩尔比为0.105∶0.231∶0.471∶0.170∶0.010∶0.013。红外光谱扫描显示EPS2、EPS3中存在糖的吡喃环、羰基、羟基等官能团。核磁共振分析表明EPS2、EPS3均为吡喃糖环,糖苷键构型既有α构型也有β构型。

关键词： 乳酸片球菌; 胞外多糖; 结构分析; 抗氧化

本文引用格式

李尧 , 卢承蓉 , 刘丹 , 韩翔鹏 , 钟青萍 . 乳酸片球菌胞外多糖的分离纯化、结构分析及抗氧化活性研究[J]. 食品与发酵工业, 2021 , 47(19) : 35 -42 . DOI: 10.13995/j.cnki.11-1802/ts.026850

Abstract

Anion exchange chromatography and molecular exclusion chromatography were used to purify the extracellular polysaccharide (EPS) from Pediococcus lactis C6. EPS1, EPS2 and EPS3 were obtained. These three components possessed antioxidant capacities with the scavenging effects on DPPH, ABTS, OH, and ·O-2. EPS3 and EPS2 presented stronger antioxidant activities than EPS1. The molecular weights of EPS2 and EPS3 were 1.90×104, and 2.53×104 Da, respectively. The ion chromatography analysis indicated that EPS2 composed of xylose, glucosamine hydrochloride, galactose and glucose, and the molar ratios were 0.912∶0.016∶0.022∶0.051. EPS3 composed of glucosamine hydrochloride, galactose, glucose, xylose, galacturonic acid and glucuronic acid, and the molar ratios were 0.105∶0.231∶0.471∶0.170∶0.010∶0.013. The infrared spectroscopy scans demonstrated that there were functional sugar groups such as pyran ring, carbonyl group and hydroxyl group in EPS2 and EPS3. The nuclear magnetic resonance analysis showed that EPS2 and EPS3 were all pyranose rings, and presented as α or β configuration.

Key words： Pediococcus lactis; extracellular polysaccharide; structure analysis; antioxidant

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