食品与发酵工业 >

2021 , Vol. 47 >Issue 19: 43 - 49

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026272

研究报告

川明参中葡聚糖的分离纯化、结构表征及抗氧化活性研究

  • 高涛 ,
  • 罗黄洋 ,
  • 吴韧 ,
  • 程飞 ,
  • 贺灵芝 ,
  • 唐玲玲 ,
  • 唐华丽 ,
  • 聂青玉
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  • 1(重庆三峡学院 生物与食品工程学院,重庆,404100)
    2(重庆市万州区食品药品检验所,重庆,404100)
    3(重庆三峡职业学院 农林科技学院,重庆,404100)
硕士研究生(唐华丽副教授为通讯作者,E-mail:hualidfood@163.com)

收稿日期: 2020-11-25

  修回日期: 2021-02-19

  网络出版日期: 2021-11-04

基金资助

重庆市教委科学技术研究项目(KJ1601008);重庆市基础科学与前沿技术研究项目(cstc2016jcyjA0522);重庆三峡学院博士研究基金项目(17ZZ02);重庆三峡学院校级重大培育项目(17ZP05)

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Purification, structure identification and antioxidant activity of glucan from Chuanminshen violaceum

  • GAO Tao ,
  • LUO Huangyang ,
  • WU Ren ,
  • CHENG Fei ,
  • HE Lingzhi ,
  • TANG Lingling ,
  • TANG Huali ,
  • NIE Qingyu
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  • 1(College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404100,China)
    2(Chongqing Wanzhou Food and Drug Inspection Institute,Chongqing 404100,China)
    3(College of Agriculture and Forestry Science,Chongqing Three Gorges Vocational College,Chongqing 404100,China)

Received date: 2020-11-25

  Revised date: 2021-02-19

  Online published: 2021-11-04

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摘要

以川明参为原料,采用超声辅助水提醇沉法提取川明参粗多糖(Chuanminshen violaceum polysaccharides,CVPs)。经凝胶柱层析后采用离子色谱、凝胶渗透色谱、紫外光谱、傅里叶红外光谱及核磁共振光谱对川明参多糖的主要组分(CVPs-III)进行结构鉴定。并采用ABTS阳离子自由基清除能力、DPPH自由基清除能力、·OH清除能力和Fe2+螯合能力为指标考察其体外抗氧化活性。结果表明,主要组分(CVPs-III)是一种分子质量为176 kDa,以→4)-Glcp-(1→4)-α-Glcp-(1→为主链,并含有少量→4)-α-Glcp-(1→3)-β-Glcp-(1→4)-α-Glcp-(1→的葡聚糖。体外抗氧化实验表明,主要组分(CVPs-III)具有较好的抗氧化活性,其ABTS阳离子自由基清除能力、DPPH自由基清除能力、Fe2+螯合能力以及·OH清除能力的半抑制浓度(IC50)分别为(1.392±0.006)、(0.596±0.017)、(0.554±0.011)和(0.618±0.018) mg/mL。

关键词： 川明参; 多糖; 葡聚糖; 结构鉴定; 抗氧化活性

本文引用格式

高涛 , 罗黄洋 , 吴韧 , 程飞 , 贺灵芝 , 唐玲玲 , 唐华丽 , 聂青玉 . 川明参中葡聚糖的分离纯化、结构表征及抗氧化活性研究[J]. 食品与发酵工业, 2021 , 47(19) : 43 -49 . DOI: 10.13995/j.cnki.11-1802/ts.026272

Abstract

The crude polysaccharides were extracted from Chuanminshen violaceum (CVPs) by ultrasonic-assisted water extraction and alcohol precipitation. After separation and purification, the structure of the main component (CVPs-III) was characterized by ion chromatography (IC), high-performance gel filter chromatography (HPGFC), ultraviolet spectrum (UV), Fourier transform infrared spectrometer (FT-IR) and nuclear magnetic resonance (NMR). And the ABTS radical scavenging ability, DPPH radical scavenging ability, hydroxyl radical scavenging capacity, Fe2+ chelating capacity and total reducing power were used to evaluate the antioxidant activity of Chuanminshen violaceum polysaccharides in vitro. The results showed that the CVPs-III was a glucan with the molecular weight of 176 kDa, and its main chain was →4)-α-Glcp-(1→4)-α-Glcp-(1→ and contains a bit of →4)-α-Glcp-(1→3)-β-Glcp-(1→4)-α-Glcp-(1→. In addition, the IC50 of its ABTS radical scavenging ability, DPPH radical scavenging ability, Fe2+ chelating capacity and hydroxyl radical scavenging ability was (1.392±0.006), (0.596±0.017), (0.554±0.011), (0.618±0.018) mg/mL, respectively.

Key words： Chuanminshen violaceum; polysaccharide; glucan; structural identification; antioxidant activity

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