食品与发酵工业 >

2021 , Vol. 47 >Issue 20: 8 - 14

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026851

研究报告

微孔板筛选酿酒酵母乙醇发酵相关性状的因素探究

  • 余鸿飞 ,
  • 姜娇 ,
  • 董琦楠 ,
  • 黄蓉 ,
  • 商华 ,
  • 叶冬青 ,
  • 刘延琳
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  • 1(西北农林科技大学 葡萄酒学院,陕西 杨凌,712100)
    2(中国葡萄酒产业技术研究院,宁夏 银川,750021)
    3(中粮长城葡萄酒(宁夏)有限公司,宁夏 永宁,750100)
    4(广西壮族自治区农业科学研究院农产品加工研究所,广西 南宁,530007)
余鸿飞(硕士研究生)和姜娇(博士研究生)为共同第一作者(叶冬青副研究员和刘延琳教授为共同通讯作者,E-mail:yedongqing@nwafu.edu.cn;yanlinliu@nwsuaf.edu.cn)

收稿日期: 2021-01-22

  修回日期: 2021-03-08

  网络出版日期: 2021-11-18

基金资助

国家重点研发计划项目(2019YFD1002500);宁夏回族自治区重大研发计划项目(2020BCF01003);国家现代农业(葡萄)产业技术体系建设专项(CARS-29-jg-03);西北农林科技大学博士科研启动费(Z1090219008)

收起

Factors affecting the selection of Saccharomyces cerevisiae with alcoholic fermentation related phenotypes via microtiter plates

  • YU Hongfei ,
  • JIANG Jiao ,
  • DONG Qinan ,
  • HUANG Rong ,
  • SHANG Hua ,
  • YE Dongqing ,
  • LIU Yanlin
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  • 1(College of Enology, Northwest A&F University, Yangling 712100, China)
    2(China Wine Industry Technology Institute, Yinchuan 750021, China)
    3(COFCO Great Wall Wine (NingXia) Co.Ltd., Yongning 750100, China)
    4(Institute of Agro-Products Processing Science and Technology,Guangxi Academy of Agricultural Sciences, Nanning 530007, China)

Received date: 2021-01-22

  Revised date: 2021-03-08

  Online published: 2021-11-18

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摘要

基于微孔板(microtiter plates,MTP)的酿酒酵母高通量筛选操作简便,节约耗材,筛选效率高,是替代传统锥形瓶静置筛选的首选方案。但由于其培养体积小,极易受其他因素影响,因此亟需探究影响MTP筛选的因素并优化筛选条件,以进一步减小误差,使结果更为可靠。该研究以多株不同发酵特性的酿酒酵母为试验材料,探究了菌株特异性、装液量及覆膜条件3个因素对酿酒酵母乙醇发酵相关性状筛选的影响。结果表明,覆膜条件是影响筛选结果的最主要因素,覆膜条件与装液量对残糖、乙醇、甘油及部分有机酸的影响显著,其影响甚至超过菌株特异性。严格厌氧覆膜、24孔板、2 mL装液量组与锥形瓶静置组在残糖、乙醇、甘油及有机酸产量上均无显著差异,具有在实际操作中替代锥形瓶静置筛选具有特定性状酿酒酵母的良好潜力。

关键词： 酿酒酵母; 微孔板; 高通量筛选; 乙醇发酵; 代谢产物; 覆膜; 装液量

本文引用格式

余鸿飞 , 姜娇 , 董琦楠 , 黄蓉 , 商华 , 叶冬青 , 刘延琳 . 微孔板筛选酿酒酵母乙醇发酵相关性状的因素探究[J]. 食品与发酵工业, 2021 , 47(20) : 8 -14 . DOI: 10.13995/j.cnki.11-1802/ts.026851

Abstract

Microtiter plate (MTP) based high through-put screening of Saccharomyces cerevisiae is the most preferred alternative to traditional conical flask static screening, since MTP method is easy operating, consumables saving, and of high efficiency. However, due to the small culture volume, application of MTP to screen yeast isolates can be easily affected by many factors. Thus, evaluation of MTP configuration is a necessity to allow more accurate yeast selection. In this study, multiple S. cerevisiae strains with varied fermentation characteristics, liquid volumes, and sealing membranes were used to assess their impact on yeast selection. Results showed that coating membrane was the most important factor affecting the screening results. Compared with strain specificity, coating membrane and liquid volume had more significant impacts on residual sugar and the yield of ethanol, glycerol, and principal organic acids. No significant differences were observed in yeast metabolites between Erlenmeyer flask ferments and 2 mL ferments in 24-well plates sealed with unbreathable membranes. This indicated that MTP method had great potential to replace conical flask static screening of S. cerevisiae strains with certain alcoholic fermentation related phenotypes.

Key words： Saccharomyces cerevisiae; microtiter plates; high-throughput screening; ethanol fermentation; metabolites; coating membrane; volume

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