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微生物合成中链二元羧酸的代谢工程研究进展

  • 李国辉 ,
  • 毛银
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  • 1(粮食发酵工艺与技术国家工程实验室(江南大学),江苏 无锡,214122)
    2(江南大学 生物工程学院,江苏 无锡,214122)
博士,助理研究员(本文通讯作者,E-mail:guohuili@jiangnan.edu.cn)

收稿日期: 2021-06-15

  修回日期: 2021-07-02

  网络出版日期: 2021-11-18

基金资助

国家重点研发计划项目(2018YFA0901400);国家自然科学基金(22008088);中国博士后科学基金资助项目(2020M681485;2021T140277);江苏省博士后科研资助计划(2020Z012)

Advances in metabolic engineering of microorganisms for medium-chain dicarboxylic acids biosynthesis

  • LI Guohui ,
  • MAO Yin
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  • 1(National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University,Wuxi 214122, China)
    2(School of Biotechnology, Jiangnan University, Wuxi 214122, China)

Received date: 2021-06-15

  Revised date: 2021-07-02

  Online published: 2021-11-18

摘要

中链二元羧酸是一类含有两个羧基的直链饱和有机酸,是制备高分子材料、橡胶、药物、染料等产品的重要原料。然而目前中链二元羧酸的工业化生产主要依赖于化学合成法,导致氮氧化物等污染物过度排放。为了实现中链二元羧酸的清洁生产,开发基于微生物发酵的生产方法迫在眉睫。以下就丁二酸、戊二酸、己二酸以及碳数更多的庚二酸、辛二酸、壬二酸、癸二酸的最新研究进展进行综述,从应用价值、传统生产方式以及生物法合成方式等角度对中链二元羧酸展开介绍,并对其今后的研究方向进行展望。

本文引用格式

李国辉 , 毛银 . 微生物合成中链二元羧酸的代谢工程研究进展[J]. 食品与发酵工业, 2021 , 47(20) : 297 -302 . DOI: 10.13995/j.cnki.11-1802/ts.028285

Abstract

As a class of linear saturated organic acids containing two carboxyl groups, medium-chain dicarboxylic acids (MCDs) play an important role as raw materials for the preparation of polymers, rubbers, pharmaceuticals, dyes and other products. However, the current industrial production of MCDs mainly relies on chemical synthesis, which leads to excessive emission of nitrogen oxides. Therefore, it is important to establish an alternative approach based on microbial fermentation to achieve the cleaner production of MCDs. In this review, the most recent research progress of succinic acid, glutaric acid, adipic acid and the medium-chain dicarboxylic acids with more carbon number such as pimelic acid, suberic acid, azelaic acid and sebacic acid are reviewed. The medium chain dicarboxylic acids are introduced from the perspective of application value, traditional production methods and biological synthesis methods, and the future directions of the research are also forecasted.

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