食品与发酵工业 >

2021 , Vol. 47 >Issue 21: 46 - 52

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026645

研究报告

胶红酵母Rhodotorula mucilaginosa CM-1菌株的鉴定及胞外多糖的分离纯化

  • 马文锦 ,
  • 李梅林 ,
  • 王博 ,
  • 周彦兵 ,
  • 张永显 ,
  • 于长青 ,
  • 彭涛
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  • (甘肃省轻工研究院有限责任公司,甘肃 兰州,730000)
博士,高级工程师(通讯作者,E-mail:mawj0707@163.com)

收稿日期: 2021-01-04

  修回日期: 2021-05-17

  网络出版日期: 2021-11-30

基金资助

甘肃省2020年度重点人才项目(2020RCXM078);西北特色农畜产品深加工国家地方联合工程研究中心(发改高技[2015]3173号);甘肃省科技计划项目(18 JR2 JA003);兰州市科技计划项目(2 019-4-41)

收起

Identification of Rhodotorula mucilaginosa CM-1 strain and isolation and purification of extracellular polysaccharide

  • MA Wenjin ,
  • LI Meilin ,
  • WANG Bo ,
  • ZHOU Yanbing ,
  • ZHANG Yongxian ,
  • YU Changqing ,
  • PENG Tao
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  • (Gansu Institute of Light Industry Limited Liability Company, Lanzhou 730000, China)

Received date: 2021-01-04

  Revised date: 2021-05-17

  Online published: 2021-11-30

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摘要

通过形态学、碳源/氮源同化实验及18S rDNA生物学方法将实验室筛选得到的菌株CM-1进行鉴定,经鉴定菌株为胶红酵母Rhodotorula mucilaginosa,对R.mucilaginosa CM-1进行无机培养基分离,纯化得到了均一、纯度高的酵母胞外多糖组分RmEPS-11,并对其进行分子质量分布、红外光谱、单糖组成及甲基化分析。结果显示,多糖组分RmEPS-11的平均分子质量为2.3×105Da,其组分是由摩尔比为1.3∶4.4∶7.8∶86.4的阿拉伯糖、半乳糖、葡萄糖和甘露糖组成。RmEPS-11的骨架由1,3-连接的甘露糖和1,2-连接的葡萄糖残基组成,分支上是1,2,6连接的半乳糖和1,2,3,4连接的阿拉伯糖,研究结果为后续胶红酵母胞外多糖的结构解析与活性分析提供了理论和数据支撑。

关键词： 胶红酵母; 菌株鉴定; 胞外多糖; 分离纯化

本文引用格式

马文锦 , 李梅林 , 王博 , 周彦兵 , 张永显 , 于长青 , 彭涛 . 胶红酵母Rhodotorula mucilaginosa CM-1菌株的鉴定及胞外多糖的分离纯化[J]. 食品与发酵工业, 2021 , 47(21) : 46 -52 . DOI: 10.13995/j.cnki.11-1802/ts.026645

Abstract

A strain CM-1 was screened and identified as Rhodotorula mucilaginosa by morphology, carbon/nitrogen source assimilation experiment and 18S rDNA sequence. A homogeneous and highly purified exopolysaccharide (RmEPS-11) was isolated and purified. The molecular weight distribution, infrared spectrum, monosaccharide composition and methylation of RmEPS-11 were analyzed. The results showed that the average molecular weight of RMPS-11 was 2.3×105 Da, and it was composed of arabinose, galactose, glucose and mannose with a molar ratio of 1.3∶4.4∶7.8∶86.4. The skeleton of RMEPS-11 was composed of 1, 3-linked mannose and 1, 2-linked glucose residues, and on the branches were 1,2,6-linked galactose and 1,2,3,4-linked arabinose. The results provide theoretical and data support for the structure analysis and activity analysis of exopolysaccharide from R. mucilaginosa.

Key words： Rhodotorula mucilaginosa; strain identification; exo-polysaccharide; isolation and purification

参考文献

[1] VALASQUES G L Jr,DE LIMA F O Jr,BOFFO E F Jr,et al.Extraction optimization and antinociceptive activity of (1→3)-β-d-glucan from Rhodotorula mucilaginosa[J].Carbohydrate Polymers,2014,105:293-299.
[2] MA W J,CHEN X F,WANG B,et al.Characterization,antioxidativity,and anti-carcinoma activity of exopolysaccharide extract from Rhodotorula mucilaginosa CICC 33013[J].Carbohydrate Polymers,2018,181:768-777.
[3] OH J,JEONG H,OH S.Characterization of optimal growth conditions and carotenoid production of strain Rhodotorula mucilaginosa HP isolated from larvae of Pieris rapae[J].Entomological Research,2009,39(6):380-387.[LinkOut]
[4] 杨铿, 杨莺莺,李卓佳,等.胶红酵母(Rhodotorula mucilaginosa)营养成分分析[J].广东农业科学,2014,41(5):146-149;154.
YANG K,YANG Y Y,LI Z J,et al.Analysis on nutrition components in Rhodotorula mucilaginosa[J].Guangdong Agricultural Sciences,2014(5):146-149;154.
[5] 冯语嫣, 刘晓凡,范青,等.葡萄抑菌附生细菌筛选鉴定及其结构表征[J].食品与发酵工业,2019,45(5):31-37.
FENG Y Y,LIU X F,FAN Q,et al.Screening,identification,and structure characterization of grape epiphytic bacteria[J].Food and Fermentation Industries,2019,45(5):31-37.
[6] 史海粟, 吴琛,陶冬冰,等.不同进口白霉奶酪中优势霉菌分离鉴定及其凝乳酶酶学特性研究[J].中国乳品工业,2019,47(7):8-13.
SHI H S,WU C,TAO D B,et al.Isolation and identification of dominant molds from different imported soft cheese with white rind and their enzymatic properties[J].China Dairy Industry,2019,47(7):8-13.
[7] 毛祥, 夏玙,张芸曌,等.四川麸醋曲药中酵母菌的分离鉴定及发酵特性[J].食品科学技术学报,2018,36(6):25-31.
MAO X,XIA Y,ZHANG Y Z,et al.Isolation,identification and fermentation characteristics of yeast from Sichuan bran vinegar starter[J].Journal of Food Science and Technology,2018,36(6):25-31.
[8] ZHANG G R,LU M,WANG J C,et al.Identification of hexose kinase genes in Kluyveromyces marxianus and thermo-tolerant one step producing glucose-free fructose strain construction[J].Scientific Reports,2017,7(1):45 104.
[9] 杜静婷, 施俊凤,贾慧文,等.适于南瓜汁原料益生酵母菌的分离鉴定及发酵工艺优化[J].食品工业科技,2020,41(17):91-97.
DU J T,SHI J F,JIA H W,et al.Isolation,identification and fermentation process optimization of probiotics for pumpkin juice[J].Science and Technology of Food Industry,2020,41(17):91-97.
[10] 曹艳, 夏其乐,陈剑兵,等.1株阿魏酸酯酶产生菌的筛选鉴定及发酵条件优化[J].微生物学杂志,2019,39(5):8-15.
CAO Y,XIA Q L,CHEN J B,et al.Screening and identification of a ferulic acid esterase producing strain and the optimization of fermentation conditions[J].Journal of Microbiology,2019,39(5):8-15.
[11] KHAW Y S,KHONG N M H,SHAHARUDDIN N A,et al.A simple 18S rDNA approach for the identification of cultured eukaryotic microalgae with an emphasis on primers[J].Journal of Microbiological Methods,2020,172:105890.
[12] 范俊英, 方慧英,诸葛斌,等.3-羟基丙酸高产菌株的筛选及诱变选育[J].微生物学通报,2012,39(9):1 355-1 362.
FAN J Y,FANG H Y,ZHUGE B,et al.Isolation,identification and mutation breeding of 3-hydroxypropionic acid high production strain[J].Microbiology China,2012,39(9):1 355-1 362.
[13] ROJO D,ZAPATA M,MAUREIRA A,et al.High-resolution melting analysis for identification of microalgae species[J].Journal of Applied Phycology,2020,32(6):3 901-3 911.
[14] 杨红燕, 严楠楠,姜艳红,等.苦荞茶多糖的体外抗氧化活性及其分离纯化[J].食品与发酵工业,2014,40(11):94-99.
YANG H Y,YAN N N,JIANG Y H,et al.Antioxidant activity in vitro and purification process of tartary buckwheat tea polysaccharide[J].Food and Fermentation Industries,2014,40(11):94-99.
[15] CHEN J J,YONG Y Y,XING M C,et al.Characterization of polysaccharides with marked inhibitory effect on lipid accumulation in Pleurotus eryngii[J].Carbohydrate Polymers,2013,97(2):604-613.
[16] ZHAO L Y,DONG Y H,CHEN G T,et al.Extraction,purification,characterization and antitumor activity of polysaccharides from Ganoderma lucidum[J].Carbohydrate Polymers,2010,80(3):783-789.
[17] 王昭晶, 曹森,曾亚威,等.血耳多糖的提取工艺、微观结构及其抗炎症作用[J].中国食品学报,2019,19(1):96-102.
WANG Z J,CAO S,ZENG Y W,et al.Extraction optimization,microstructure and anti-inflammatory properties of a polysaccharide from Tremella samguinea Peng[J].Journal of Chinese Institute of Food Science and Technology,2019,19(1):96-102.
[18] 巴尼特J A.酵母菌的特征与鉴定手册[M].青岛:青岛海洋大学出版社,1991.
BARNETT J A.The Characteristics and Identification Manual of Yeasts[M].Qingdao:Qingdao Ocean University Press,1991.
[19] FELL J W,BOEKHOUT T,FONSECA A,et al.Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis[J].International Journal of Systematic and Evolutionary Microbiology,2000,50(3):1 351-1 371.
[20] LI Q,YU N W,WANG Y P,et al.Extraction optimization of Bruguiera gymnorrhiza polysaccharides with radical scavenging activities[J].Carbohydrate Polymers,2013,96(1):148-155.
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