食品与发酵工业 >

2021 , Vol. 47 >Issue 21: 109 - 114

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026566

研究报告

银杏肽对急性酒精性肝损伤小鼠的保护作用

  • 郑义 ,
  • 李诗颖 ,
  • 糜心怡 ,
  • 陈琳 ,
  • 李闯 ,
  • 梁一凡
展开
  • 1(徐州工程学院 食品与生物工程学院,江苏 徐州,221018)
    2(江苏省食品资源开发与质量安全重点建设实验室,江苏 徐州,221018)
副教授(通讯作者,E-mail:biozheng@gmail.com)

收稿日期: 2020-12-26

  修回日期: 2021-06-24

  网络出版日期: 2021-11-30

基金资助

徐州市科技项目重点研发计划(KC19122)

收起

Protective effect of Ginkgo biloba peptides on acute alcoholic liver injury in mice

  • ZHENG Yi ,
  • LI Shiying ,
  • MI Xinyi ,
  • CHEN Lin ,
  • LI Chuang ,
  • LIANG Yifan
Expand
  • 1(School of Food and Biological Engineering, Xuzhou University of Technology, Xuzhou 221018, China)
    2(Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou 221018, China)

Received date: 2020-12-26

  Revised date: 2021-06-24

  Online published: 2021-11-30

Fold

摘要

摄入生物活性肽,可缓解酒精代谢诱导的氧化应激和炎性损伤,因此被认为是一种有效的辅助治疗急性酒精性肝损伤的策略。基于急性酒精性肝损伤小鼠模型考察了银杏肽(Ginkgo biloba peptides, GBP)的肝保护作用。将雄性昆明小鼠随机分为正常组、模型组、GBP低剂量(50 mg/kg)、GBP中剂量(100 mg/kg)、GBP高剂量组(200 mg/kg)和阳性对照组(100 mg/kg还原型谷胱甘肽),通过灌胃酒精建立急性酒精性肝损伤小鼠模型,测定小鼠血清丙氨酸氨基转移酶(alanine aminotransferase, ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase, AST)、甘油三酯(triglyceride, TG)、总胆固醇(total cholesterol, TC)、IL-1β、IL-6和TNF-α水平,肝脏过氧化氢酶(catalase, CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)和总超氧化物歧化酶(total superoxide dismutase, T-SOD)活性,肝脏丙二醛(malondialdehyde, MDA)和蛋白质羰基(protein carbonyl group, PCG)水平。结果表明,GBP能降低酒精性肝损伤小鼠血清中ALT、AST、TG和TC水平,抑制促炎细胞因子IL-1β、IL-6和TNF-α水平,提高肝脏CAT、GSH-Px和T-SOD活性,降低肝脏MDA和PCG水平。GBP通过抗氧化和抗炎途径发挥其对酒精性肝损伤小鼠的保护作用。

关键词： 银杏肽; 急性酒精性肝损伤; 肝保护; 抗氧化; 抗炎

本文引用格式

郑义 , 李诗颖 , 糜心怡 , 陈琳 , 李闯 , 梁一凡 . 银杏肽对急性酒精性肝损伤小鼠的保护作用[J]. 食品与发酵工业, 2021 , 47(21) : 109 -114 . DOI: 10.13995/j.cnki.11-1802/ts.026566

Abstract

Intake of bioactive peptides has been proposed as an effective adjuvant therapy for acute alcoholic liver injury by ameliorating alcohol-induced oxidative stress and inflammatory damage. This paper investigated the hepatoprotective effect of Ginkgo biloba peptides (GBP) on acute alcoholic liver injury in mice. Male Kunming mice were randomly divided into six groups, including normal group, model group, GBP low-dose (50 mg/kg), GBP medium-dose (100 mg/kg), GBP high-dose group (200 mg/kg) and positive control group (100 mg/kg reduced glutathione). An acute alcoholic liver injury mice mode was established by ethanol exposure. The kits were employed to determine the levels of the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α). And the levels of the liver tissue catalase (CAT), glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD), malondialdehyde (MDA) and protein carbonyl group (PCG). The results showed that GBP treatment reduced the levels of ALT, AST, TG and TC, down-regulated the levels of pro-inflammatory cytokines including IL-1β, IL-6 and TNF-α. Besides, GBP treatment also increased the CAT, GSH-Px and T-SOD activities and decreased MDA and PCG levels. The results indicated that GBP exerted the hepatoprotective effect on acute alcoholic liver injury in mice through antioxidant and anti-inflammatory pathways.

Key words： Ginkgo biloba peptides; acute alcoholic liver injury; hepatoprotective; antioxidant; anti-inflammatory

参考文献

[1] SÁNCHEZ A,VÁZQUEZ A.Bioactive peptides:A review[J].Food Quality and Safety,2017,1(1):29-46.
[2] LORENZO J M,MUNEKATA P E S,GÓMEZ B,et al.Bioactive peptides as natural antioxidants in food products-A review[J].Trends in Food Science & Technology,2018,79:136-147.
[3] CICERO A F G,FOGACCI F,COLLETTI A.Potential role of bioactive peptides in prevention and treatment of chronic diseases:A narrative review[J].British Journal of Pharmacology,2017,174(11):1 378-1 394.
[4] KORHONEN H,PIHLANTO A.Bioactive peptides:Production and functionality[J].International Dairy Journal,2006,16(9):945-960.
[5] MAZORRA-MANZANO M A,RAMíREZ-SUAREZ J C,YADA R Y.Plant proteases for bioactive peptides release:A review[J].Critical Reviews in Food Science and Nutrition,2018,58(13): 2 147-2 163.
[6] 杨月欣. 中国食物成分表:第一册[M].第6版.北京:北京大学医学出版社,2018.
YANG Y X.China Food Composition:Book 1[M].6th Edition.Beijing:Peking University Medical Press,2018.
[7] 黄文. 白果活性蛋白的分离、纯化、结构及其生物活性研究[D].武汉:华中农业大学,2002.
HUANG W.Studies on separation purification and structure of Ginkgo seed protein and its biologic activities[D].Wuhan:Huazhong Agricultural University,2002.
[8] HUANG W,DENG Q C,XIE B J,et al.Purification and characterization of an antioxidant protein from Ginkgo biloba seeds[J].Food Research International,2010,43(1):86-94.
[9] 邓乾春, 汪兰,吴佳,等.一种白果清蛋白的抗衰老活性研究[J].中国药理学通报,2006,22(3):352-357.
DENG Q C,WANG L,WU J,et al.Studies on anti-aging activity of Ginkgo Albumin Protein (GAP)[J].Chinese Pharmacological Bulletin,2006,22(3):352-357.
[10] GAO N N,WADHWANI P,MÜHLHÄUSER P,et al.An antifungal protein from Ginkgo biloba binds actin and can trigger cell death[J].Protoplasma,2016,253(4):1 159-1 174.
[11] 邓乾春, 段会轲,谢笔钧,等.白果清蛋白对免疫功能低下小鼠的调节作用[J].食品科学,2006,27(6):195-199.
DENG Q C,DUAN H K,XIE B J,et al.Regulating function of Ginkgo Albumin Protein (GAP) on immunosuppressive model in mice[J].Food Science,2006,27(6):195-199.
[12] 邓乾春, 黄文,谢笔钧.白果清蛋白抑制肿瘤活性及其机制的初步研究[J].营养学报,2006,28(3):259-262.
DENG Q C,HUANG W,XIE B J.The preliminary studies on anti-tumor activity of ginkgo albumin and its mechanism[J].Acta Nutrimenta Sinica,2006,28(3):259-262.
[13] 贾韶千, 吴彩娥,范龚健,等.双酶法制备银杏抗氧化肽工艺研究[J].食品科学,2011,32(21):201-206.
JIA S Q,WU C E,FAN G J,et al.Preparation of antioxidant peptides derived from Ginkgo biloba kernel by dual-enzymatic method[J].Food Science,2011,32(21):201-206.
[14] 张焕新, 臧大存,刘靖,等.银杏肽的抗氧化性研究[J].食品研究与开发,2008,29(12):27-29.
ZHANG H X,ZANG D C,LIU J,et al.Antioxidant activity of Ginkgo peptides[J].Food Research and Development,2008,29(12):27-29.
[15] 张灿, 吴彩娥,范龚健,等.酶解银杏蛋白制备α-葡萄糖苷酶抑制肽的研究[J].食品与机械,2016,32(11):137-141.
ZHANG C,WU C E,FAN G J,et al.Preparation of α-glucosidase inhibitory peptides derived from Ginkgo biloba by enzymatic method[J].Food & Machinery,2016,32(11):137-141.
[16] MA F F,WANG H,WEI C K,et al.Three novel ACE inhibitory peptides isolated from Ginkgo biloba seeds:Purification,inhibitory kinetic and mechanism[J].Frontiers in Pharmacology,2019,9:1 579.
[17] MASARONE M,ROSATO V,DALLIO M,et al.Epidemiology and natural history of alcoholic liver disease[J].Reviews on Recent Clinical Trials,2016,11(3):167-174.
[18] WANG W J,XIAO P,XU H Q,et al.Growing burden of alcoholic liver disease in China:A review[J].World Journal of Gastroenterology,2019,25(12):1 445-1 456.
[19] WANG H J,GAO B,ZAKHARI S,et al.Inflammation in alcoholic liver disease[J].Annual Review of Nutrition,2012,32(1):343-368.
[20] ALBANO E.Oxidative mechanisms in the pathogenesis of alcoholic liver disease[J].Molecular Aspects of Medicine,2008,29(1):9-16.
[21] MA Z L,HOU T,SHI W,et al.Inhibition of hepatocyte apoptosis:An important mechanism of corn peptides attenuating liver injury induced by ethanol[J].International Journal of Molecular Sciences,2015,16(9):22 062-22 080.
[22] WU Y H,PAN X C,ZHANG S X,et al.Protective effect of corn peptides against alcoholic liver injury in men with chronic alcohol consumption:A randomized double-blind placebo-controlled study[J].Lipids in Health and Disease,2014,13:192.
[23] LIN B,ZHANG F,YU Y C,et al.Marine collagen peptides protect against early alcoholic liver injury in rats[J].The British Journal of Nutrition,2012,107(8):1 160-1 166.
[24] 刘睿, 珠娜,刘欣然,等.核桃低聚肽对急性酒精性肝损伤大鼠保护作用[J].中国公共卫生,2020,36(2):192-195.
LIU R,ZHU N,LIU X R,et al.Protective effect of walnut oligopeptides on acute alcohol-induced liver injury in rats[J].Chinese Journal of Public Health,2020,36(2):192-195.
[25] WEI C K,THAKUR K,LIU D H,et al.Enzymatic hydrolysis of flaxseed (Linum usitatissimum L.) protein and sensory characterization of Maillard reaction products[J].Food Chemistry,2018,263:186-193.
[26] SONG X L,LIU Z H,ZHANG J J,et al.Antioxidative and hepatoprotective effects of enzymatic and acidic-hydrolysis of Pleurotus geesteranus mycelium polysaccharides on alcoholic liver diseases[J].Carbohydrate Polymers,2018,201:75-86.
[27] WANG M C,ZHU P L,JIANG C X,et al.Preliminary characterization,antioxidant activity in vitro and hepatoprotective effect on acute alcohol-induced liver injury in mice of polysaccharides from the peduncles of Hovenia dulcis[J].Food and Chemical Toxicology,2012,50(9):2 964-2 970.
[28] ZHENG Y,CUI J,CHEN A H,et al.Optimization of ultrasonic-microwave assisted extraction and hepatoprotective activities of polysaccharides from Trametes orientalis[J].Molecules,2019,24(1):147.
[29] ZHOU C Y,LAI Y L,HUANG P,et al.Naringin attenuates alcoholic liver injury by reducing lipid accumulation and oxidative stress[J].Life Sciences,2019,216:305-312.
[30] ZAKHARI S.Overview:How is alcohol metabolized by the body?[J].Alcohol Research & Health,2006,29(4):245-254.
[31] CEDERBAUM A I.Alcohol metabolism[J].Clinics in Liver Disease,2012,16(4):667-685.
[32] TESCHKE R.Alcoholic liver disease:Alcohol metabolism,cascade of molecular mechanisms,cellular targets,and clinical aspects[J].Biomedicines,2018,6(4):106.
[33] OSNA N A,DONOHUE T M,KHARBANDA K K.Alcoholic liver disease:Pathogenesis and current management[J].Alcohol Research,2017,38(2):147-161.
[34] LAI Y L,ZHOU C Y,HUANG P,et al.Polydatin alleviated alcoholic liver injury in zebrafish larvae through ameliorating lipid metabolism and oxidative stress[J].Journal of Pharmacological Sciences,2018,138(1):46-53.
[35] AN L,WANG X D,CEDERBAUM A I.Cytokines in alcoholic liver disease[J].Archives of Toxicology,2012,86(9):1 337-1 348.
[36] GAO B.Hepatoprotective and anti-inflammatory cytokines in alcoholic liver disease[J].Journal of Gastroenterology and Hepatology,2012,27:89-93.
[37] CICHOŻ-LACH H,MICHALAK A.Oxidative stress as a crucial factor in liver diseases[J].World Journal of Gastroenterology,2014,20(25):8 082-8 091.
[38] LI S,TAN H Y,WANG N,et al.The role of oxidative stress and antioxidants in liver diseases[J].International Journal of Molecular Sciences,2015,16(11):26 087-26 124.
[39] DALLE-DONNE I,ROSSI R,GIUSTARINI D,et al.Protein carbonyl groups as biomarkers of oxidative stress[J].Clinica Chimica Acta,2003,329(1-2):23-38.
[40] TUMA D J.Role of malondialdehyde-acetaldehyde adducts in liver injury[J].Free Radical Biology and Medicine,2002,32(4):303-308.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司