食品与发酵工业 >

2021 , Vol. 47 >Issue 21: 115 - 122

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028393

研究报告

普洱茶多糖对健康小鼠短链脂肪酸代谢与肠道菌群组成的调节作用

  • 许凌凌 ,
  • 程旺开 ,
  • 周小楠
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  • 1(芜湖职业技术学院 生物工程学院,安徽 芜湖,241002)
    2(芜湖市生命健康工程技术研究中心,安徽 芜湖,241000)
    3(皖南医学院 基础医学院,安徽 芜湖,241003)
硕士,副教授(通讯作者,E-mail:xulingling261@163.com)

收稿日期: 2021-06-17

  修回日期: 2021-07-10

  网络出版日期: 2021-11-30

基金资助

安徽省教育厅高校优秀青年人才支持计划重点项目(gxyqZD2018101);芜湖职业技术学院校级自然科学研究重点项目(wzyzrzd202112)

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Regulatory effect of Pu-erh tea polysaccharide on short-chain fatty acid metabolism and gut microbiota in mice

  • XU Lingling ,
  • CHENG Wangkai ,
  • ZHOU Xiaonan
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  • 1(Biological Engineering Department, Wuhu Institute of Technology, Wuhu 241002, China)
    2(Life and Health Engineering Research Center of Wuhu, Wuhu 241000, China)
    3(Wannan Medical College, School of Basic Medicine, Wuhu 241003, China)

Received date: 2021-06-17

  Revised date: 2021-07-10

  Online published: 2021-11-30

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摘要

为探究普洱茶多糖对健康小鼠肠道菌群组成的影响,并预测其代谢功能,以健康小鼠连续灌胃35 d,收集小鼠粪便进行分析。结果表明,普洱茶多糖能显著增加小鼠粪便中短链脂肪酸(乙酸、丙酸、丁酸和戊酸)的含量,但存在剂量依赖性。16S rRNA测序表明,补充300 mg/kg体重的普洱茶多糖能有效增加健康小鼠肠道菌群的多样性,并影响特定细菌的相对丰度。与对照组相比,粪杆菌属、拟普雷沃菌属、阿克曼氏菌属和另枝菌属被鉴定为高剂量普洱茶多糖组的生物标志物。此外,利用基于标记基因序列来预测功能丰度(phylogenetic investigation of communities by reconstruction of unobserved states,PICRUSt2),结果表明氨基酸代谢、能量代谢和其他次生代谢产物生物合成是高剂量普洱茶多糖组中主要富集的KEGG通路。阿克曼氏菌属与不饱和脂肪酸生物合成及类固醇生物合成呈正相关,与酮体的合成和降解呈负相关。研究结果表明,适量补充普洱茶多糖可以改变肠道菌群的组成,增加短链脂肪酸含量,从而改善肠道代谢功能。

关键词： 普洱茶; 多糖; 16S rRNA高通量测序; 肠道菌群; 短链脂肪酸

本文引用格式

许凌凌 , 程旺开 , 周小楠 . 普洱茶多糖对健康小鼠短链脂肪酸代谢与肠道菌群组成的调节作用[J]. 食品与发酵工业, 2021 , 47(21) : 115 -122 . DOI: 10.13995/j.cnki.11-1802/ts.028393

Abstract

To explore the effect of Pu-erh tea polysaccharide (PEP) on gut microbiota and predict metabolic function, healthy mice were gavaged with PEP for 35 days. The results showed that PEP could remarkably increase the levels of short-chain fatty acids (acetic acid, propionic acid, butyric acid and valeric acid) in the feces with dose-dependent matter. 300 mg/kg body weight PEP was effective in increasing diversity of the gut microbiota and affected the relative abundance of specific bacteria. Faecalibaculum, Alloprevotella, Akkermansia and Alistipes were identified as the biomarkers of the PEP with high dose group. In addition, amino acid metabolism, energy metabolism and biosynthesis of other secondary metabolites were the primary enriched phenotypic KEGG pathways in the PEP with high dose group. Akkermansia positively correlated with biosynthesis of unsaturated fatty acids and steroid, and negatively correlated with synthesis and degradation of ketone bodies. These results suggested that the appropriate amount of PEP supplementation could enhance the content of short-chain fatty acids by altering the composition of gut microbiota and improve metabolic function.

Key words： Pu-erh tea; polysaccharide; 16S rRNA high-throughput sequencing; gut microbiota; short-chain fatty acid

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