食品与发酵工业 >

2021 , Vol. 47 >Issue 22: 115 - 120

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027211

研究报告

非靶向代谢组技术研究饮料发酵前后物质变化

  • 张妍 ,
  • 赵昕琪 ,
  • 王天琪 ,
  • 王成 ,
  • 于冰 ,
  • 吕佳璐 ,
  • 张莉力 ,
  • 许云贺
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  • (锦州医科大学 食品科学与工程学院,辽宁 锦州,121000)
硕士研究生(张莉力教授和许云贺教授为共同通讯作者,E-mail: 13634967549@163.com;sn_97@126.com)

收稿日期: 2021-03-04

  修回日期: 2021-04-14

  网络出版日期: 2021-12-16

基金资助

辽宁省高等学校创新人才支持计划(2020);辽宁省自然科学基金项目(2019-ZD-0600)

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Component changes in corn beverage before and after fermentation analyzed by non-targeted metabolomics technique

  • ZHANG Yan ,
  • ZHAO Xinqi ,
  • WANG Tianqi ,
  • WANG Cheng ,
  • YU Bing ,
  • LYU Jialu ,
  • ZHANG Lili ,
  • XU Yunhe
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  • (School of Food Science and Engineering, Jinzhou Medical University, Jinzhou 121000, China)

Received date: 2021-03-04

  Revised date: 2021-04-14

  Online published: 2021-12-16

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摘要

为了解玉米及其副产物发酵饮料的主要代谢成分,代谢通路及物质积累。以2种乳酸菌为发酵剂混合发酵的玉米及其副产物饮料为研究对象,利用非靶向代谢组技术对饮料发酵前后小分子量物质变化进行了对比分析,结果发现,玉米及其副产物饮料发酵前后存在较大的代谢物差异。筛选出14种差异代谢物,主要包括核苷酸及其衍生物3种,有机酸2种,氨基酸及其衍生物2种,黄酮类2种,苯丙酸1种,抗生素1种,烟酰胺类1种,萘酚类衍生物1种,磷酸甲基醇类1种。其中包括具有抗炎活性的萘酚类物质1-羟基-2-萘甲酸;具有抗病菌,降血压,抗真菌,抗氧化,抗艾滋病病毒,抗肿瘤等作用的异戊烯;具有抗氧化作用,对心脑血管也有防治作用,同时能预防肿瘤的表儿茶素等。通过KEGG(Kyoto Encyclopedia of Genes and Genomes)代谢通路分析发现,5条代谢通路影响较显著,分别为缬氨酸,亮氨酸和异亮氨酸的生物合成、酪氨酸代谢、双组分系统、嘌呤代谢、柠檬酸循环。确定了乳酸菌发酵能够促进玉米副产物发酵饮料代谢产物的合成和累积,为乳酸菌用于发酵谷物饮料的综合评价利用提供参考。

关键词： 代谢组学; 乳酸菌; 发酵; 玉米及其副产物

本文引用格式

张妍 , 赵昕琪 , 王天琪 , 王成 , 于冰 , 吕佳璐 , 张莉力 , 许云贺 . 非靶向代谢组技术研究饮料发酵前后物质变化[J]. 食品与发酵工业, 2021 , 47(22) : 115 -120 . DOI: 10.13995/j.cnki.11-1802/ts.027211

Abstract

To understand the main metabolic substrates, metabolic pathways, accumulated products and byproducts of mixed fermentations of corn, two lactic acid bacteria strains were used as mixed fermentation starters to ferment corn beverages and byproducts. The low molecular weight substance content before and after beverage fermentation was assessed and compared using non-targeted metabolomics technology. Significant differences in metabolites were observed between the pre-fermented corn and its fermentation product beverage. Fourteen differential metabolites were identified, including three nucleotides and their derivatives, two organic acids, two amino acids and their derivatives, two flavonoids, one phenylpropionic acid, one antibiotic, one nicotinamide, one naphthol derivative, and one phosphate methyl alcohol. These included multiple potentially beneficial activities: 1-hydroxy-2-naphthoic acid, a naphthol with anti-inflammatory activity, isopentene, which has anti-bacterial, hypotensive, anti-fungal, anti-oxidant, anti-HIV, anti-tumor, and other effects, and epicatechin, which has the function of anti-oxidation, prevention and cure to heart and cerebrovascular, and can prevent the epicatechin of tumor, etc. Metabolic pathway analysis via KEGG revealed five metabolic pathways significantly involved in fermentation, including valine, leucine and isoleucine biosynthesis, tyrosine metabolism, two-component system, purine metabolism, and the citric acid cycle. These studies confirmed that lactic acid bacteria fermentation could promote synthesis and accumulation of useful metabolites as byproducts of corn-based beverage fermentation, and provide a reference for the comprehensive evaluation of the utilization of lactic acid bacteria in fermented cereal beverage production.

Key words： metabolomics; lactic acid bacteria; fermentation; corn and its by-products

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