食品与发酵工业 >

2021 , Vol. 47 >Issue 24: 193 - 200

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027350

生产与科研应用

一株产胞外多糖微藻的分离鉴定及其多糖抗氧化活性的研究

  • 吴思伟 ,
  • 李思雨 ,
  • 孙寒 ,
  • 刘红全 ,
  • 何秀苗 ,
  • 黄莹 ,
  • 吴嘉惠 ,
  • 黄柳媚 ,
  • 龙寒
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  • (广西民族大学 海洋与生物技术学院,广西多糖材料与改性重点实验室,广西 南宁,530006)
硕士研究生(刘红全副教授为通讯作者,E-mail:lhongquan@163.com)

收稿日期: 2021-03-15

  修回日期: 2021-03-30

  网络出版日期: 2022-01-21

基金资助

广西自然科学基金项目(2018GXNSFAA294032);广西民族大学研究生教育创新计划项目(gxun-chxps201919)

收起

Isolation and identification of an exopolysaccharide-producing microalgae strain and its antioxidant activity

  • WU Siwei ,
  • LI Siyu ,
  • SUN Han ,
  • LIU Hongquan ,
  • HE Xiumiao ,
  • HUANG Ying ,
  • WU Jiahui ,
  • HUANG Liumei ,
  • LONG Han
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  • (Guangxi Key Laboratory of Polysaccharide Materials and Modification, College of Marine and Biotechnology, Guangxi University for Nationalities, Nanning 530006, China)

Received date: 2021-03-15

  Revised date: 2021-03-30

  Online published: 2022-01-21

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摘要

从广西北部湾防城港红树林筛选到1株高产胞外多糖的微藻GF02,结合形态学与分子生物学鉴定为小球藻属Chlorella Sorokiniana。初步表征结果表明GF02胞外粗多糖以α-型吡喃糖为主,通过响应面法优化GF02产胞外多糖的条件,实验表明,GF02产胞外多糖最佳培养条件为NaNO3 2.1 g/L,Na2CO3 0.02 g/L,MgSO4 0.086 g/L,K2HPO4 0.06 g/L,在此条件下,其胞外多糖积累量为216.268 mg/L,是优化前的1.60倍。对胞外多糖抗氧化活性进行了初步的研究,抗氧化活性结果表明,GF02粗多糖对DPPH清除率达到36.53%,对羟基自由基清除率达到67.33%。该研究为小球藻多糖的制备和进一步开发利用提供了实验基础。

关键词： 小球藻; 胞外多糖; 分离鉴定; 培养条件; 抗氧化活性

本文引用格式

吴思伟 , 李思雨 , 孙寒 , 刘红全 , 何秀苗 , 黄莹 , 吴嘉惠 , 黄柳媚 , 龙寒 . 一株产胞外多糖微藻的分离鉴定及其多糖抗氧化活性的研究[J]. 食品与发酵工业, 2021 , 47(24) : 193 -200 . DOI: 10.13995/j.cnki.11-1802/ts.027350

Abstract

A microalgae strain with high exopolysaccharides production isolated from mangrove in Fangchenggang city, Beibu Gulf region, Guangxi province was named as GF02. GF02 was identified as Chlorella sorokiniana according to morphology and molecular biology analysis. Preliminary characterization results showed that the main component of the extracellular polysaccharide was α-pyranose. Response surface method was used to optimize the culture conditions of GF02 for the extracellular polysaccharide production. The results showed that the optimal culture condition for GF02 to produce exopolysaccharides were NaNO3 2.1 g/L,Na2CO3 0.02 g/L,MgSO4 0.086 g/L and K2HPO4 0.06 g/L. Under these conditions, the accumulation of extracellular polysaccharides was 216.268 mg/L, which was 1.60 times of that before optimization. The results of antioxidant activity analysis showed that the eliminating rate of GF02 exopolysaccharides on DPPH and hydroxyl radicals were up to 36.53% and 67.33%, respectively. This study would provide experimental basis for the preparation and further utilization of Chlorella polysaccharides.

Key words： Chlorella sorokiniana; extracellular polysaccharide; isolation and identification; culture condition; antioxidant activity

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