食品与发酵工业 >

2022 , Vol. 48 >Issue 1: 117 - 123

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028972

研究报告

体外仿生消化对金银花醇提物成分及抗氧化活性的影响

  • 孙纯勇 ,
  • 王玲娜 ,
  • 颜培正 ,
  • 张永清 ,
  • 李佳 ,
  • 赵东升
展开
  • (山东中医药大学 药学院,山东 济南,250355)
硕士研究生(李佳教授和赵东升讲师为共同通信作者,E-mail:ljytl7172@163.com;dongshengpw2008@163.com)

收稿日期: 2021-08-10

  修回日期: 2021-09-18

  网络出版日期: 2022-01-27

基金资助

国家重点研发计划项目(2017YFC1701503);国家自然科学基金项目(81903750,82004233);山东省重大科技创新工程项目(2018CXGC0309);山东省中医药科技发展计划项目(2019-0030);山东中医药大学中药资源保护与质量评价青创团队支持项目

收起

Effects of biomimetic digestion on composition and antioxidant activity of alcohol extracts from Lonicera japonica Thunb. in vitro

  • SUN Chunyong ,
  • WANG Lingna ,
  • YAN Peizheng ,
  • ZHANG Yongqing ,
  • LI Jia ,
  • ZHAO Dongsheng
Expand
  • (School of Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

Received date: 2021-08-10

  Revised date: 2021-09-18

  Online published: 2022-01-27

Fold

摘要

为探究金银花醇提物在体外仿生消化中成分含量及抗氧化活性变化规律,通过测定模拟胃肠消化前后金银花醇提物中12种成分含量、总体抗氧化能力、羟自由基(·OH)清除率和DPPH自由基清除率,并进行成分含量和抗氧化活性变化相关性分析。结果显示,酚酸类成分含量随模拟胃消化时间延长而降低,而在模拟肠消化过程中无显著性变化(P>0.05)。黄酮类成分含量整体呈先上升后下降趋势,而在模拟肠消化过程中无显著性变化(P>0.05)。仿生消化过程中环烯醚萜类成分含量无显著性变化(P>0.05)。经模拟胃肠消化后,消化产物总体抗氧化能力及对·OH和DPPH自由基清除率均显著降低(P<0.05),并与酚酸和黄酮含量呈显著正相关(P<0.05)。胃肠道环境均能促进金银花中酚酸和黄酮类成分降解,并显著降低其抗氧化活性,为进一步研究金银花体内胃肠消化提供一定的理论参考。

关键词： 金银花; 醇提物; 化学成分; 仿生消化; 抗氧化活性

本文引用格式

孙纯勇 , 王玲娜 , 颜培正 , 张永清 , 李佳 , 赵东升 . 体外仿生消化对金银花醇提物成分及抗氧化活性的影响[J]. 食品与发酵工业, 2022 , 48(1) : 117 -123 . DOI: 10.13995/j.cnki.11-1802/ts.028972

Abstract

The aim of this study was to explore the changes of chemical components and antioxidant activities in alcohol extracts from Lonicera japonica Thunb. during biomimetic digestion in vitro. There were twelve compounds in alcohol extract from L.japonica, and the following index which including total antioxidant capacity, hydroxyl radical scavenging rate, and DPPH radical scavenging rate before and after gastrointestinal digestion were determined. The results showed that the contents of phenolic acid were decreased with the extension of simulated gastric digestion time, but there was no significant change in simulated intestinal digestion (P>0.05). The contents of flavonoids increased at first and then decreased, but there was no significant change in the simulated intestinal digestion process (P>0.05). However, before and after bionic digestion, no significant change was found in the content of iridoids in garland (P>0.05). After simulated stomach and intestinal digestion, the total antioxidant capacity and the scavenging rate to hydroxyl radical (·OH) and ·DPPH radical of the digested products were significantly decreased (P<0.05) which with a strong correlation (P<0.05). The gastric and intestinal environment can promote the degradation of phenolic acids and flavonoids in L.japonica, and significantly reduce their antioxidant activity. This study provides theoretical reference for further research on the digestive process of L.japonica in vivo.

Key words： Lonicera japonica Thunb.; alcohol extract; chemical component; biomimetic digestion; antioxidant activity

参考文献

[1] 国家药典委员会.中华人民共和国药典:一部[S].北京:中国医药科技出版社,2020.
Chnese Plamacopoeia Comrission.Plamacopoeia of the people’s republic of China[S].Beijing:China Medical Science Press,2020.
[2] PAN H Q,ZHOU H,MIAO S,et al.An integrated approach for global profiling of multi-type constituents:Comprehensive chemical characterization of Lonicerae japonicae Flos as a case study[J].Journal of Chromatography A,2020,1 613:460674.
[3] 朱文卿,任汉书,郑媛媛,等.金银花的功能性成分及其生物活性研究进展[J].食品工业科技,2021,42(13):412-426.
ZHU W Q,REN H S,ZHENG Y Y,et al.Research progress in functional components and bioactivity of honeysuckle[J].Science and Technology of Food Industry,2021,42(13):412-426.
[4] 伍鹏,王晶晶,董志忠,等.体外仿生消化系统的研究进展—从静态到动态[J].生物产业技术,2019(6):26-41.
WU P,WANG J J,DONG Z Z,et al.Advance in biomimetic in vitro digestion systems-from static to dynamic[J].Biotechnology & Business,2019(6):26-41.
[5] 李诺,张东杰,张桂芳,等.体外模拟消化技术研究进展[J].食品与机械,2021,37(3):201-206.
LI N,ZHANG D J,ZHANG G F,et al.Advances in the study of in vitro simulated degistion[J].Food & Machinery,2021,37(3):201-206.
[6] 刘静敏,史静兰,鲁江,等.体外模拟胃肠消化过程中四种茶叶活性成分及抗氧化性变化规律[J].食品工业科技,2020,41(1):301-306.
LIU J M,SHI J L,LU J,et al.Changes of active constituents and antioxidant properties of four kinds of tea during gastrointestinal digestion in vitro[J].Science and Technology of Food Industry,2020,41(1):301-306.
[7] 李贻,贺君,张鹏敏.体外模拟胃肠道消化下刺梨抗氧化成分的释放[J].现代食品科技,2020,36(2):102-107.
LI Y,HE J,ZHANG P M.Antioxidant components release of Rosa roxburghii Tratt by in vitro simulated gastrointestinal digestion[J].Modern Food Science and Technology,2020,36(2):102-107.
[8] 陈希苗,李美英,许秋莉,等.体外模拟胃肠消化中山楂多酚及抗氧化活性的变化[J].食品科学,2019,40(5):31-37.
CHEN X M,LI M Y,XU Q L,et al.Changes in polyphenol contents and antioxidant activity in hawthorn(Crataegus pinnatifida Bunge) during simulated gastrointestinal digestion[J].Food Science,2019,40(5):31-37.
[9] 刘豪,张冬青,刘硕,等.金银花不同提取物抗氧化活性的研究[J].食品研究与开发,2016,37(1):48-52.
LIU H,ZHANG D Q,LIU S,et al.Study on the antioxidant activity of different extracts of the flower buds of Lonicera japonica[J].Food Research and Development,2016,37(1):48-52.
[10] 王桂林,徐未芳,刘乐,等.金银花不同溶剂提取物多酚含量及抗氧化活性[J].食品研究与开发,2020,41(5):104-107.
WANG G L,XU W F,LIU L,et al.The content and antioxidant activities of honeysuckle polyphenol extracted by different solvent[J].Food Research and Development,2020,41(5):104-107.
[11] 张敏敏,赵志国,刘倩,等.基于离线2D-HPLC-DPPH-ESI-Q-TOF/MS联用技术的金银花抗氧化成分系统筛选研究[J].中草药,2021,52(11):3 193-3 200.
ZHANG M M,ZHAO Z G,LIU Q,et al.Rapid discovery of trace antioxidants in Lonicerae Japonicae Flos by 2D-HPLC-DPPH-ESI-TOF/MS[J].Chinese Traditional and Herbal Drugs,2021,52(11):3 193-3 200.
[12] 宋亚玲,王红梅,倪付勇,等.金银花中酚酸类成分及其抗炎活性研究[J].中草药,2015,46(4):490-495.
SONG Y L,WANG H M,NI F Y,et al.Study on anti-inflammatory activities of phenolic acids from Lonicerae Japonicae Flos[J].Chinese Traditional and Herbal Drugs,2015,46(4):490-495.
[13] DE TAGLIAZUCCHI D,VERZELLONI E,DE BERTOLINI D,et al.In vitro bio-accessibility and antioxidant activity of grape polyphenols[J].Food Chemistry,2010,120(2):599-606.
[14] MINEKUS M,ALMINGER M,ALVITO P,et al.A standardised static in vitro digestion method suitable for food-an international consensus[J].Food & Function,2014,5(6):1 113-1 124.
[15] WANG L N,ZHANG Y Q.Quality analysis and high-performance liquid chromatographic fingerprint analysis of new cultivated kind of Lonicerae japonicae Flos “Hua Jin 6” from different harvest times[J].Chinese Medicine,2017,8(1):18-32.
[16] 王谢祎,翟宇鑫,李倩,等.南酸枣在模拟消化过程中抗氧化活性及多酚含量分析[J].食品科学,2016,37(11):7-11.
WANG X Y,ZHAI Y X,LI Q,et al.Changes in antioxidant activity and phenol content in Choerospondias axillaris fruits during simulated gastrointestinal digestion[J].Food Science,2016,37(11):7-11.
[17] 赵玉静,李建宽,张鑫,等.番石榴叶黄酮类化学成分及其抗氧化活性研究[J].中国中药杂志,2018,43(4):760-765.
ZHAO Y J,LI J K,ZHANG X,et al.Study on antioxidant activity of flavonoids from leaves of Psidium guajava[J].China Journal of Chinese Materia Medica,2018,43(4):760-765.
[18] 熊文,吴婷,曹伟伟,等.体外消化对藜蒿叶多酚含量及抗氧化活性的影响[J].现代食品科技,2020,36(1):78-83;287.
XIONG W,WU T,CAO W W,et al.Effects of in vitro digestion on phenolic acid content and antioxidant activity of Artemisia selengensis Turcz leaves[J].Modern Food Science and Technology,2020,36(1):78-83;287.
[19] 万坤,郭珍妮,李思杰,等.体外消化对高粱壳酚类物质含量及其抗氧化活性的影响[J].食品科技,2017,42(3):180-185.
WAN K,GUO Z N,LI S J,et al.Effects of simulated digestion on contents and antioxidants of phenolics of sorghum hull[J].Food Science and Technology,2017,42(3):180-185.
[20] 李如蕊,陈欣,茹月蓉,等.体外模拟消化过程中核桃花提取物抗氧化活性的变化[J].现代食品科技,2020,36(11):196-201;169.
LI R R,CHEN X,RU Y R,et al.Changes of in vitro simulated gastrointestinal digestion on the antioxidant activity of walnut flower[J].Modern Food Science and Technology,2020,36(11):196-201;169.
[21] 张露,杨斯涵,涂宗财,等.体外模拟消化对鸡爪槭叶多酚稳定性及其自由基清除和α-葡萄糖苷酶抑制活性的影响[J].天然产物研究与开发,2018,30(3):354-361.
ZAHNG L,YANG S H,TU Z C,et al.Influence of in vitro simulated digestion on stability and free radical scavenging and α-glucosidase inhibitory activities of Acer palmatum leaves polyphenols[J].Natural Product Research and Development,2018,30(3):354-361.
[22] 徐文流,贝琦,梁诗雅,等.红曲霉发酵提高金银花的抗氧化活性[J].现代食品科技,2020,36(9):47-53;163.
XU W L,BEI Q,LIANG S Y,et al.Improvement of the antioxidative activities of Flos Lonicerae japonicae through its solid-state fermentation with Monascus[J].Modern Food Science and Technology,2020,36(9):47-53;163.
[23] 付晶晶,肖海芳,宋元达.金银花等6种植物提取物总黄酮含量与抗氧化性相关性研究[J].食品与机械,2017,33(6):159-163.
FU J J,XIAO H F,SONG Y D.Acomparative study of contents of total flavonoids and their antioxidant activities in six plants[J].Food & Machinery,2017,33(6):159-163.
[24] 朱小峰,朱晓娣,王金梅.封丘产金银花不同提取物的体外抗氧化、抗凝血及α-葡萄糖苷酶抑制活性研究[J].中国药房,2016,27(34):4 804-4 806.
ZHU X F,ZHU X D,WANG J M.Study on in vitro antioxidant,anticoagulant and α-glucosidase inhibitory activities of different extracts of Lonicerae japonicae from Fengqiu[J].China Pharmacy,2016,27(34):4 804-4 806.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司