研究报告

奶酪中乳酸菌的分离筛选及其对小清蛋白的降解性能初步评价

  • 李晓晨 ,
  • 王进 ,
  • 徐晨晨 ,
  • 肖叶 ,
  • 李燕 ,
  • 卢瑛 ,
  • 李晓晖
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  • 1(上海海洋大学 食品学院,上海,201306)
    2(上海水产品及加工及贮藏工程技术研究中心,上海,201306)
    3(农业部水产品贮藏保鲜质量安全风险评估实验室,上海,201306)
硕士研究生(卢瑛教授和李晓晖副教授为共同通信作者,E-mail:y-lu@shou.edu.cn;xhli@shou.edu.cn)

收稿日期: 2021-03-08

  修回日期: 2021-04-09

  网络出版日期: 2022-02-28

基金资助

国家重点研发计划资助(2019YFD0902003);上海市科技兴农重点攻关项目[沪农科攻字(2016)第4-4号]

Isolation and screening of lactic acid bacteria from cheese and its degradability evaluation to parvalbumin

  • LI Xiaochen ,
  • WANG Jin ,
  • XU Chenchen ,
  • XIAO Ye ,
  • LI Yan ,
  • LU Ying ,
  • LI Xiaohui
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  • 1(College of Food, Shanghai Ocean University, Shanghai 201306, China)
    2(Shanghai Aquatic Product and Processing and Storage Engineering Technology Research Center, Shanghai 201306, China)
    3(Laboratory for Risk Assessment of Quality and Safety of Storage and Preservation of Aquatic Products, Ministry of Agriculture, Shanghai 201306, China)

Received date: 2021-03-08

  Revised date: 2021-04-09

  Online published: 2022-02-28

摘要

为筛选可降解鱼类主要过敏原小清蛋白(parvalbumin,PV)的乳酸菌,该研究以产蛋白酶作为初筛标准从奶酪中分离筛选获得8株乳酸菌,经16S rDNA测序鉴定为Lactobacillus plantarum 4株,Lactobacillus casei 2株,Enterococcus faecalis 2株。利用这8株乳酸菌对草鱼中提取富集的PV溶液进行孵育,SDS-PAGE分析发现Lactobacillus casei B-2和Lactobacillus plantarum B-17孵育后的PV含量显著减少,进一步研究这2株乳酸菌对PV免疫活性的影响作用,结果发现这2株菌孵育PV溶液36 h后,PV发生降解,其含量显著下降,且免疫活性分别下降了96.5% 和84.8%。由此可见,这2株乳酸菌具有降低鱼类主要过敏原致敏性的潜在能力,为今后乳酸菌发酵鱼肉开发低致敏鱼肉制品提供了科学依据。

本文引用格式

李晓晨 , 王进 , 徐晨晨 , 肖叶 , 李燕 , 卢瑛 , 李晓晖 . 奶酪中乳酸菌的分离筛选及其对小清蛋白的降解性能初步评价[J]. 食品与发酵工业, 2022 , 48(2) : 65 -70 . DOI: 10.13995/j.cnki.11-1802/ts.027284

Abstract

To screen the lactic acid bacteria that can degrade the main fish allergen, parvalbumin (PV), eight lactic acid bacteria strains were isolated and identified by 16S rDNA sequencing from cheese, including Lactobacillus plantarum (four strains), Lactobacillus casei (two strains) and Enterococcus faecalis (two strains). These eight strains of lactic acid bacteria were incubated with the enriched PV extraction from Ctenopharyngodon idella. According to the SDS-PAGE analysis, it was found that the PV content decreased significantly when incubated with Lactobacillus casei B-2 and Lactobacillus plantarum B-17 strains. The effect of these two strains on the immunoactivity of PV was further studied. Our results showed that PV was degraded obviously after incubation with B-2 and B-17 strains for 36 h, and the content of PV decreased significantly. Also, the immunological activity decreased by 96.5% and 84.8% respectively. Therefore, these two strains of lactic acid bacteria have the potential to reduce the allergenicity of the main allergen PV of fish, which provides a scientific basis for the development of low allergenic fish products by lactic acid bacteria fermentation in the future.

参考文献

[1] 伍恒, 钱和, 汪何雅, 等.发酵法对降低食品过敏原的作用[J].食品工业科技, 2011, 32(8):453-457.
WU H, QIAN H, WANG H Y, et al.A summary on removing the allergen from food by fermentation[J].Science and Technology of Food Industry, 2011, 32(8):453-457.
[2] FRIAS J, SONG Y S, MARTÍNEZ-VILLALUENGA C, et al.Immunoreactivity and amino acid content of fermented soybean products[J].Journal of Agricultural & Food Chemistry, 2008, 56(1):99-105.
[3] 肖叶, 叶精勤, 李晓晨, 等.植物乳杆菌(Lactobacillus plantarum)对过敏原原肌球蛋白免疫活性的消减作用[J].食品科学, 2021,42(18):95-102.
XIAO Y, YE J Q, LI X C, et al.Study on the reduction effect of Lactobacillus plantarum on the immunological activity of tropomyosin[J].Food Science, 2021,42(18):95-102.
[4] TZVETKOVA I, DALGALARRONDO M, DANOVA S, et al.Hydrolysis of major dairy proteins by lactic acid bacteria from Bulgarian yogurts[J].Journal of Food Biochemistry, 2007, 31(5):680-702.
[5] KLEBER N, WEYRICH U, HINRICHS J.Screening for lactic acid bacteria with potential to reduce antigenic response of β-lactoglobulin in bovine skim milk and sweet whey[J].Innovative Food Science&Emerging Technologies, 2006, 7(3):233-238.
[6] 杨华, 刘卫兵, 伯慧敏.慢性荨麻疹370例体外变应原检测分析[J].中国医学文摘(皮肤科学), 2009, 26(5):273-275.
YANG H, LIU W B, BO H M.Investigation of allergens in 370 patients with chronic urticaria[J].China Medical Abstract of Dermatology, 2009, 26(5):273-275.
[7] KOBAYASHI Y, YANG T, YU C T, et al.Quantification of major allergen parvalbumin in 22 species of fish by SDS-PAGE[J].Food Chemistry, 2016, 194:345-353.
[8] KUBOTA H, KOBAYASHI A, KOBAYASHI Y, et al.Reduction in IgE reactivity of Pacific mackerel parvalbumin by heat treatment[J].Food Chemistry, 2016, 206:78-84.
[9] 国家卫生和计划生育委员会, 国家食品药品监督管理总局.GB 4789.35—2016食品微生物学检验 乳酸菌检验[S].北京:中国标准出版社, 2017.
National Health and Family Planning Commission, China Food and Drug Administration.GB 4789.35—2016 Food microbiological inspection lactic acid bacteria test[S].Beijing:Standards Press of China, 2017.
[10] 胡玲萍, 裘迪红, 王鑫钰.泡菜中高产蛋白酶乳酸菌的筛选鉴定及其酶学特性初步研究[J].食品与发酵工业, 2015, 41(6):35-40.
HU L P, QIU D H, WANG X Y.Screening and identification of lactic acid bacteria with high productivity of protease in pickled vegetable and studies on the enzymology characteristics of the protease[J].Food and Fermentation Industries, 2015, 41(6):35-40.
[11] 郭艳荣, 党娜, 苏馨, 等.埃塞俄比亚发酵食品中乳酸菌的分离鉴定[J].中国乳品工业, 2020, 48(5):15-19.
GUO Y R, DANG N, SU X, et al.Isolation and identification of lactic acid bacteria from fermented foods in Ethiopian[J].China Dairy Industry, 2020, 48(5):15-19.
[12] 呼斯楞, 刘红新, 于洁, 等.内蒙古呼伦贝尔地区传统发酵乳中乳酸菌的多样性分析[J].微生物学通报, 2016, 43(5):984-990.
HU S L, LIU H X, YU J, et al.Diversity of lactic acid bacteria associated with traditional fermented milk from Hulun Buir in Inner Mongolia[J].Microbiology, 2016, 43(5):984-990.
[13] 周滟晴, 刘婷, 周婉婷, 等.高产酸果醋醋酸菌的筛选鉴定及其耐醇和耐温性探究[J].食品与发酵工业, 2021,47(10):72-78.
ZHOU Y Q, LIU T, ZHOU W T, et al.Isolation and identification of an acetic acid bacteria with high acid yield and research on tolerance to ethanol and temperature[J].Food and Fermentation Industries, 2021,47(10):72-78.
[14] ZHANG X P, LI Y R, TAO Y, et al.A novel method based on infrared spectroscopic inception-resnet networks for the detection of the major fish allergen parvalbumin[J].Food Chemistry, 2021, 337:127986.
[15] SONG Y N, LI Z X, LIN H, et al.Effect of malondialdehyde treatment on the IgE binding capacity and conformational structure of shrimp tropomyosin[J].Food Chemistry, 2015, 175:374-380.
[16] PESCUMA M, HÉBERT E M, HAERTLÉ T, et al.Lactobacillus delbrueckii sub sp.bulgaricus CRL 454 cleaves allergenic peptides of β-lactoglobulin[J].Food Chemistry, 2015, 170:407-414.
[17] 丁婕, 王梦梦, 汪立平, 等.鼠李糖乳酸杆菌对河豚肝脏中河豚毒素毒性的影响[J].食品科学, 2019, 40(19):237-241.
DING J, WANG M M, WANG L P, et al.Effect of Lactobacillus rhamnosus on the toxicity of tetrodotoxin in the liver of puffer fish[J].Food Science, 2019, 40(19):237-241.
[18] SHARP M F, STEPHEN J N, KRAFT L, et al.Immunological cross-reactivity between four distant parvalbumins-impact on allergen detection and diagnostics[J].Molecular Immunology, 2015, 63(2):437-448.
[19] KAWASE S, USHIO H, OHSHIMA T, et al.Preparation of monoclonal antibodies against tuna parvalbumin[J].Fisheries Science, 2001, 67(3):559-561.
[20] DO CARMO A, DA SILVA D, DE OLIVEIRA M, et al.Genes involved in protein metabolism of the probiotic lactic acid bacterium Lactobacillus delbrueckii UFV H2b20[J].Beneficial Microbes, 2011, 2(3):209-220.
[21] HEBERT E M, MAMONE G, PICARIELLO G, et al.Characterization of the pattern of α s1-and β-casein breakdown and release of a bioactive peptide by a cell envelope proteinase from Lactobacillus delbrueckii subsp.lactis CRL 581[J].Applied and Environmental Microbiology, 2008, 74(12):3 682-3 689.
[22] ANGGRAINI H, TONGKHAO K, CHANPUT W.Reducing milk allergenicity of cow, buffalo, and goat milk using lactic acid bacteria fermentation[R].Malang:8th Annual Basic Science International Conference (BaSIC)-Coverage of Basic Sciences toward the World's Sustainability Challanges, 2018.
[23] KLICHE T, LI B, BOCKELMANN W, et al.Screening for proteolytically active lactic acid bacteria and bioactivity of peptide hydrolysates obtained with selected strains[J].Applied Microbiology and Biotechnology, 2017, 101(20):7 621-7 633.
[24] LAW J, HAANDRIKMAN A.Proteolytic enzymes of lactic acid bacteria[J].International Dairy Journal, 1997, 7(1):1-11.
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