食品与发酵工业 >

2022 , Vol. 48 >Issue 2: 189 - 197

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.027695

研究报告

黄刺浆果不同组分多糖理化性质及生物活性的比较

  • 周雯 ,
  • 韩丽娟 ,
  • 马娜娜 ,
  • 漆莹 ,
  • 岳庆明 ,
  • 索南卓玛 ,
  • 郭妍 ,
  • 院珍珍
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  • 1(青海大学 农牧学院,青海 西宁,810016)
    2(青海大学省部共建三江源生态与高原农业国家重点实验室,青海 西宁,810016)
硕士研究生(韩丽娟副教授为通信作者,E-mail:hlj880105@163.com)

收稿日期: 2021-04-12

  修回日期: 2021-05-10

  网络出版日期: 2022-02-28

基金资助

青藏高原黄刺多糖精细结构解析及其干预胰岛β细胞凋亡作用机制研究(31960088);基于胰岛β细胞凋亡相关信号通路探讨黄刺浆果多糖降血糖作用机制(2020-ZJ-951Q)

收起

Comparative study on the physicochemical properties and biological activities of polysaccharides from different components in the berry of Berberi dasystachya

  • ZHOU Wen ,
  • HAN Lijuan ,
  • MA Nana ,
  • QI Ying ,
  • YUE Qingming ,
  • SUO Nanzhuoma ,
  • GUO Yan ,
  • YUAN Zhenzhen
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  • 1(College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China)
    2(State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China)

Received date: 2021-04-12

  Revised date: 2021-05-10

  Online published: 2022-02-28

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摘要

综合体外抗氧化、降血糖及对肠道微生物的调节作用全面探究不同组分黄刺多糖(Berberi dasystachya polysaccharides,BDPs)的降血糖能力。采用分级醇沉制备不同组分BDPs:BDPs-70、BDPs-80及BDPs-90,通过高效凝胶渗透色谱、傅里叶红外光谱、扫描电镜表征其初级结构,比较DPPH自由基和ABTS阳离子自由基清除率、还原力、α-淀粉酶和α-葡萄糖苷酶抑制率及其对嗜酸乳杆菌、大肠杆菌的影响。结果表明,BDPs-80的糖醛酸含量最高,BDPs-70、BDPs-80、BDPs-90重均分子质量分别为9 851、9 901、9 855 Da,且具有多糖特征吸收峰,为β-吡喃型多糖,表面结构均呈片状。3种多糖对DPPH自由基、ABTS阳离子自由基的清除率依次为:BDPs-90>BDPs-80>BDPs-70,BDPs-80的还原能力最优;对α-淀粉酶的抑制率:BDPs-90>BDPs-80>BDPs-70;对α-葡萄糖苷酶的抑制率:BDPs-80>BDPs-90>BDPs-70。3种多糖作为碳源均能促进嗜酸乳杆菌增殖(BDPs-90的促进效果显著),显著提高短链脂肪酸含量,抑制大肠杆菌生长(BDPs-80的抑制效果最好)。该研究可为将黄刺多糖开发成为预防和治疗糖尿病的食品及医药提供理论基础。

关键词： 黄刺多糖; 分级醇沉; 抗氧化; 降血糖; 肠道微生物

本文引用格式

周雯 , 韩丽娟 , 马娜娜 , 漆莹 , 岳庆明 , 索南卓玛 , 郭妍 , 院珍珍 . 黄刺浆果不同组分多糖理化性质及生物活性的比较[J]. 食品与发酵工业, 2022 , 48(2) : 189 -197 . DOI: 10.13995/j.cnki.11-1802/ts.027695

Abstract

The hypoglycemic effects of different components of Berberi dasystachya polysaccharides (BDPs) were studied comprehensively by combining the effects of antioxidation, hypoglycemia and regulation of intestinal flora in vitro. Different components of BDPs(BDPs-70, BDPs-80 and BDPs-90)were prepared by ethanol alcohol precipitation and their primary structures were characterized by high performance gel permeation chromatography, Fourier transform infrared spectroscopy and scanning electron microscopy. Comparing DPPH and ABTS free radical scavenging rate, reducing power, α-amylase and α-glucosidase inhibition rate and their effects on Lactobacillus acidophilus and Escherichia coli were compared. The results showed that the uronic acid content of BDPs-80 was the highest, and the average molecular weights of BDPs-70, BDPs-80 and BDPs-90 were 9 851, 9 901 and 9 855 Da, respectively. It showed characteristic absorption peaks of polysaccharides, which were β-pyran polysaccharides with flaky surface structure. The scavenging rates of the three polysaccharides on DPPH and ABTS free radicals were in the following order: BDPs-90>BDPs-80>BDPs-70, and the reducing power of BDPs-80 was the best. The inhibition rate of α-amylase was BDPs-90>BDPs-80>BDPs-70. The inhibition rate of α-glucosidase was BDPs-80>BDPs-90>BDPs-70. The three polysaccharides as carbon sources can promote the proliferation of Lactobacillus acidophilus (BDPs-90 has the best promotion effect), significantly increase the content of short chain fatty acids, and inhibit the growth of Escherichia coli (BDPs-80 has the best inhibitory effect). The theoretical basis for the development of Berberi dasystachya polysaccharides into food and medicine for the prevention and treatment of diabetes were provided in this research.

Key words： Berberi dasystachya polysaccharides; ethanol fractional precipitation; antioxidant activity; hypoglycemic effect; gut microbes

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