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铁蛋白纳米笼分子装载途径及食品活性物质递送的研究进展

  • 陈海 ,
  • 马良 ,
  • 戴宏杰 ,
  • 付余 ,
  • 王洪霞 ,
  • 张宇昊
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  • (西南大学 食品科学学院,重庆,400715)
博士,副教授(张宇昊教授为通信作者,E-mail:zhy1203@163.com)

收稿日期: 2021-04-25

  修回日期: 2021-05-26

  网络出版日期: 2022-03-04

基金资助

中央高校基本科研业务费专项资金项目(SWU120055);国家自然科学基金项目(31671881;31972102)

Encapsulation strategies of ferritin and its application for food bioactive compounds delivery: A review

  • CHEN Hai ,
  • MA Liang ,
  • DAI Hongjie ,
  • FU Yu ,
  • WANG Hongxia ,
  • ZHANG Yuhao
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  • (College of Food Science,Southwest University,Chongqing 400715,China)

Received date: 2021-04-25

  Revised date: 2021-05-26

  Online published: 2022-03-04

摘要

铁蛋白(ferritin)是一种广泛存在于动物、植物和微生物中的多亚基笼形结构蛋白,具有调节体内铁代谢平衡的功能,同时可以保护细胞免受因各种环境胁迫而导致的细胞氧化损伤。近年来,随着研究的深入,铁蛋白独特的纳米笼形结构以及特殊的理化性质使其成为一种具有广泛应用前景的新型蛋白质纳米载体材料。文章对铁蛋白的分子结构和功能进行了简要阐述,介绍了铁蛋白纳米颗粒的制备方法,总结了铁蛋白装载外源性小分子的基本途径:基于可逆组装特性的分子装载途径和基于环境响应的通道“门控”特性的分子装载途径;并综述了铁蛋白作为纳米载体在食品生物活性物质应用中的最新研究进展,以期为铁蛋白纳米载体的开发及在食品领域的应用提供研究思路。

本文引用格式

陈海 , 马良 , 戴宏杰 , 付余 , 王洪霞 , 张宇昊 . 铁蛋白纳米笼分子装载途径及食品活性物质递送的研究进展[J]. 食品与发酵工业, 2022 , 48(3) : 304 -310 . DOI: 10.13995/j.cnki.11-1802/ts.027868

Abstract

Ferritin is a multi-subunit protein nanocage that is widely distributed in many types of organisms from microbes to plants and animals. Its function mainly involves the regulation of iron metabolism, as well as cell protection from oxidative damage caused by various environmental stresses. In recent years, with a deep understanding of ferritin protein, it has been considered as a novel protein nano-carrier that can be applied in multiple disciplines due to the unique cage-like structure and specific characteristics of ferritin nanocage. Herein, the distribution, structure, function and preparation of ferritin nanocage have been introduced and followed by an overview of the molecular encapsulation strategy of ferritin nanocages, including the reversible disassembly/reassembly property mediated approach and stimulus-responsive channel-mediated approach. Finally, the applications of ferritin as a nano-carrier for food bioactive compounds encapsulation were reviewed. The purpose of this review was to provide research ideas for the preparation and development of ferritin nano-carrier and its application in food science.

参考文献

[1] MING T H,HUAN H S,SU C,et al.Structural comparison of two ferritins from the marine invertebrate Phascolosoma esculenta[J].FEBS Open Bio,2021,11(3):793-803.
[2] MELMAN A,BOU-ABDALLAH F.Iron mineralization and core dissociation in mammalian homopolymeric H-ferritin:Current understanding and future perspectives[J].Biochimica et Biophysica Acta General Subjects,2020,1864(11):129700.
[3] LV C,YIN S H,ZHANG X Q,et al.16-mer ferritin-like protein templated gold nanoclusters for bioimaging detection of methylmercury in the brain of living mice[J].Analytica Chimica Acta,2020,1127:149-155.
[4] YANG L,FAN D W,ZHANG Y,et al.Ferritin-based electrochemiluminescence nanosurface energy transfer system for procalcitonin detection using HWRGWVC heptapeptide for site-oriented antibody immobilization[J].Analytical Chemistry,2019,91(11):7 145-7 152.
[5] YAO H C,ZHAO W W,ZHANG S G,et al.Dual-functional carbon dot-labeled heavy-chain ferritin for self-targeting bio-imaging and chemo-photodynamic therapy[J].Journal of Materials Chemistry B,2018,6(19):3 107-3 115.
[6] SITIA L,BONIZZI A,MAZZUCCHELLI S,et al.Selective targeting of cancer-associated fibroblasts by engineered H-ferritin nanocages loaded with navitoclax[J].Cells,2021,10(2):328.
[7] LIU Y Q,YANG R,LIU J G,et al.Fabrication,structure,and function evaluation of the ferritin based nano-carrier for food bioactive compounds[J].Food Chemistry,2019,299:125097.
[8] ZHANG N,YU X Q,XIE J X,et al.New insights into the role of ferritin in iron homeostasis and neurodegenerative diseases[J].Molecular Neurobiology,2021,58(6):2 812-2 823.
[9] PULLIN J,BRADLEY J M,MOORE G R,et al.Electron transfer from haem to the di-iron ferroxidase centre in bacterioferritin[J].Angewandte Chemie International Edition,2021,60(15):8 376-8 379.
[10] CHASTEEN N D,HARRISON P M.Mineralization in ferritin:An efficient means of iron storage.[J].Journal of Structural Biology,1999,126(3):182-194.
[11] YANG R,TIAN J,LIU Y Q,et al.Interaction mechanism of ferritin protein with chlorogenic acid and iron ion:The structure,iron redox,and polymerization evaluation[J].Food Chemistry,2021,349:129144.
[12] 付晓苹,云少君,赵广华.植物铁蛋白的铁氧化沉淀与还原释放机理[J].农业生物技术学报,2014,22(2):239-248.
FU X P,YUN S J,ZHAO G H.Mechanisms of iron oxidative deposition and iron reductive release in phytoferritin[J].Journal of Agricultural Biotechnology,2014,22(2):239-248.
[13] 辛敏,黄昀,郝鹏飞,等.鹰嘴豆铁蛋白提取工艺优化研究[J].天然产物研究与开发,2014,26(2):283-288.
XIN M,HUANG Y,HAO P F,et al.Optimization of extraction conditions of ferritin from chickpea seed[J].Natural Product Research and Development,2014,26(2):283-288.
[14] 饶承冬,叶浪,邓静,等.响应面法优化鲟鱼肝脏铁蛋白提取工艺[J].四川农业大学学报,2018,36(6):851-856.
RAO C D,YE L,DENG J,et al.Optimization of extraction process of ferritin from sturgeon liver by response surface methodology[J].Journal of Sichuan Agricultural University,2018,36(6):851-856.
[15] SONG X T,ZHENG Y X,ZHU L,et al.Development of robust and facile purification process for production of recombinant human ferritin heavy chain nanoparticle from Escherichia coli[J].Process Biochemistry,2021,104(5):1-9.
[16] SU C,MING T H,WU Y,et al.Crystallographic characterization of ferritin from Sinonovacula constricta[J].Biochemical and Biophysical Research Communications,2020,524(1):217-223.
[17] LI H,ZANG J C,TAN X Y,et al.Purification and characterizations of a nanocage ferritin GF1 from oyster (Crassostrea gigas)[J].LWT,2020,127:109416.
[18] DONG X B,TANG B,LI J,et al.Expression and purification of intact and functional soybean (Glycine max) seed ferritin complex in Escherichia coli[J].Journal of Microbiology and Biotechnology,2008,18(2):299-307.
[19] 夏小雨,李晗,王震宇,等.人源重链铁蛋白纯化及其纳米粒制备[J].食品科学,2020,41(12):91-98.
XIA X Y,LI H,WANG Z Y,et al.Purification and preparation of nanoparticles of human H-chain ferritin[J].Food Science,2020,41(12):91-98.
[20] MAITY B,LI Z P,NIWASE K,et al.Single-molecule level dynamic observation of disassembly of the apo-ferritin cage in solution[J].Physical Chemistry Chemical Physics,2020,22(33):18 562-18 572.
[21] SHAMSI A,SHAHWAN M,KHAN M S,et al.Elucidating the interaction of human ferritin with quercetin and naringenin:Implication of natural products in neurodegenerative diseases:Molecular docking and dynamics simulation insight[J].ACS Omega,2021,6(11):7 922-7 930.
[22] MANSOURIZADEH F,ALBERTI D,BITONTO V,et al.Efficient synergistic combination effect of Quercetin with Curcumin on breast cancer cell apoptosis through their loading into Apo ferritin cavity[J].Colloids and Surfaces.B,Biointerfaces,2020,191:110982.
[23] ZHANG T,LV C,CHEN L L,et al.Encapsulation of anthocyanin molecules within a ferritin nanocage increases their stability and cell uptake efficiency[J].Food Research International,2014,62:183-192.
[24] CHEN L L,BAI G L,YANG R,et al.Encapsulation of β-carotene within ferritin nanocages greatly increases its water-solubility and thermal stability[J].Food Chemistry,2014,149:307-312.
[25] CHEN H,ZHANG S,XU C,et al.Engineering protein interfaces yields ferritin disassembly and reassembly under benign experimental conditions[J].Chemical Communications(Cambridge England),2016,52(46):7 402-7 405.
[26] WANG W M,WANG L L,LI G B,et al.AB loop engineered ferritin nanocages for drug loading under benign experimental conditions[J].Chemical Communications (Cambridge,England),2019,55(82):12 344-12 347.
[27] LIAO X Y,YUN S J,ZHAO G H.Structure,function,and nutrition of phytoferritin:A newly functional factor for iron supplement[J].Critical Reviews in Food Science and Nutrition,2014,54(10):1 342-1 352.
[28] YANG R,LIU Y Q,MENG D M,et al.Alcalase enzymolysis of red bean (adzuki) ferritin achieves nanoencapsulation of food nutrients in a mild condition[J].Journal of Agricultural and Food Chemistry,2018,66(8):1 999-2 007.
[29] MIRMOGHTADAIE L,SHOJAEE ALIABADI S,HOSSEINI S M.Recent approaches in physical modification of protein functionality[J].Food Chemistry,2016,199:619-627.
[30] YANG R,LIU Y Q,MENG D M,et al.Effect of atmospheric cold plasma on structure,activity,and reversible assembly of the phytoferritin[J].Food Chemistry,2018,264:41-48.
[31] MENG D M,WANG B W,ZHEN T Y,et al.Pulsed electric fields-modified ferritin realizes loading of rutin by a moderate pH transition[J].Journal of Agricultural and Food Chemistry,2018,66(46):12 404-12 411.
[32] MENG D M,ZUO P,SONG H L,et al.Influence of manothermosonication on the physicochemical and functional properties of ferritin as a nanocarrier of iron or bioactive compounds[J].Journal of Agricultural and Food Chemistry,2019,67(23):6 633-6 641.
[33] YANG R,LIU Y Q,MENG D M,et al.Urea-driven epigallocatechin gallate (EGCG) permeation into the ferritin cage,an innovative method for fabrication of protein-polyphenol Co-assemblies[J].Journal of Agricultural and Food Chemistry,2017,65(7):1 410-1 419.
[34] YANG R,LIU Y Q,BLANCHARD C,et al.Channel directed rutin nano-encapsulation in phytoferritin induced by guanidine hydrochloride[J].Food Chemistry,2018,240:935-939.
[35] YANG R,TIAN J,LIU Y Q,et al.Thermally induced encapsulation of food nutrients into phytoferritin through the flexible channels without additives[J].Journal of Agricultural and Food Chemistry,2017,65(46):9 950-9 955.
[36] 张晨曦,张晓荣,吕晨艳,等.超声辅助法制备铁蛋白-虾青素包埋物[J].食品科学,2020,42(11):94-101.
ZHANG C X,ZHANG X R,LYU C Y.et al.Ultrasound-assisted encapsulation of astaxanthin within ferritin nanocages with enhanced efficiency[J].Food Science,2020,42(11):94-101.
[37] BAYAT P,FARSHCHI M,YOUSEFIAN M,et al.Flavonoids,the compounds with anti-inflammatory and immunomodulatory properties,as promising tools in multiple sclerosis (MS) therapy:A systematic review of preclinical evidence[J].International Immunopharmacology,2021,95:107562.
[38] DE CARVALHO A P A,CONTE C A Jr.Health benefits of phytochemicals from Brazilian native foods and plants:Antioxidant,antimicrobial,anti-cancer,and risk factors of metabolic/endocrine disorders control[J].Trends in Food Science & Technology,2021,111:534-548.
[39] YANG R,SUN G Y,ZHANG M,et al.Epigallocatechin gallate (EGCG) decorating soybean seed ferritin as a rutin nanocarrier with prolonged release property in the gastrointestinal tract[J].Plant Foods for Human Nutrition,2016,71(3):277-285.
[40] YANG R,GAO Y,ZHOU Z,et al.Fabrication and characterization of ferritin-chitosan-lutein shell-core nanocomposites and lutein stability and release evaluation in vitro[J].RSC Advances,2016,6(42):35 267-35 279.
[41] DREWNOWSKI A,GOMEZ-CARNEROS C.Bitter taste,phytonutrients,and the consumer:A review[J].The American Journal of Clinical Nutrition,2000,72(6):1 424-1 435.
[42] LI H,TAN X Y,XIA X Y,et al.Improvement of thermal stability of oyster (Crassostrea gigas) ferritin by point mutation[J].Food Chemistry,2021,346:128879.
[43] ZHANG X R,ZANG J C,CHEN H,et al.Thermostability of protein nanocages:the effect of natural extra peptide on the exterior surface[J].RSC Advances,2019,9(43):24 777-24 782.
[44] YANG R,ZHOU Z K,SUN G Y,et al.Synthesis of homogeneous protein-stabilized rutin nanodispersions by reversible assembly of soybean (Glycine max) seed ferritin[J].RSC Advances,2015,5(40):31 533-31 540.
[45] LV C,ZHAO G H,LÖNNERDAL B.Bioavailability of iron from plant and animal ferritins[J].The Journal of Nutritional Biochemistry,2015,26(5):532-540.
[46] ZHANG Y,DONG Y X,LI X,et al.Proanthocyanidin encapsulated in ferritin enhances its cellular absorption and antioxidant activity[J].Journal of Agricultural and Food Chemistry,2019,67(41):11 498-11 507.
[47] MONTEMIGLIO L C,TESTI C,CECI P,et al.Cryo-EM structure of the human ferritin-transferrin receptor 1 complex[J].Nature Communications,2019,10(1):1121.
[48] RENSI N,SAMMARCO A,MOCCIA V,et al.Evaluation of TFR-1 expression in feline mammary cancer and in vitro antitumor efficacy study of doxorubicin-loaded H-ferritin nanocages[J].Cancers,2021,13(6):1 248.
[49] PANDOLFI L,BELLINI M,VANNA R,et al.H-ferritin enriches the curcumin uptake and improves the therapeutic efficacy in triple negative breast cancer cells[J].Biomacromolecules,2017,18(10):3 318-3 330.
[50] MENG D M,CHEN S N,LIU J,et al.Double-interface binding of two bioactive compounds with cage-like ferritin[J].Journal of Agricultural and Food Chemistry,2020,68(29):7 779-7 788.
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