食品与发酵工业 >

2022 , Vol. 48 >Issue 4: 226 - 234

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.026592

研究报告

白茶多酚对D-半乳糖致小鼠肾损伤模型的改善作用

  • 李冲 ,
  • 杨悦 ,
  • 骞宇 ,
  • 赵欣
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  • 1(重庆第二师范学院重庆市功能性食品协同创新中心,重庆,400067)
    2(重庆第二师范学院重庆市功能性食品工程技术研究中心,重庆,400067)
    3(重庆第二师范学院功能性食品研发重庆市工程实验室,重庆,400067)
    4(重庆第二师范学院 生物与化学工程学院,重庆,400067)
硕士,实验师(赵欣教授为通信作者,E-mail:zhaoxin@cque.edu.cn)

收稿日期: 2020-12-30

  修回日期: 2021-03-19

  网络出版日期: 2022-03-16

基金资助

重庆第二师范学院校级科研项目资助(KY201921C);重庆市高校创新研究群体项目(CXQTP20033)

收起

Effect of polyphenols in white tea on kidney injury induced by D-galactose in mice

  • LI Chong ,
  • YANG Yue ,
  • QIAN Yu ,
  • ZHAO Xin
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  • 1(Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China)
    2(Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China)
    3(Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing 400067, China)
    4(College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, China)

Received date: 2020-12-30

  Revised date: 2021-03-19

  Online published: 2022-03-16

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摘要

该研究建立D-半乳糖诱导小鼠肾损伤模型,用不同剂量[250、500 mg/(kg·d)]白茶多酚灌胃小鼠42 d,于第6周末测量小鼠肾组织中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)、总抗氧化能力(total antioxidant capacity,T-AOC)、一氧化氮(nitric oxide,NO)、丙二醛(malondialdehyde,MDA)和细胞因子IL-6、IL-10、TNF-α、IFN-β、IFN-γ的含量。组织学分析肾脏切片病理变化,体外抗氧化试验考察白茶多酚对DPPH自由基、ABTS阳离子自由基清除能力以及肾脏器官系数的分析。此外,通过HPLC分析白茶多酚的生物活性成分。结果表明,白茶多酚显著(P<0.05)提高CAT、GSH-Px、T-AOC、IL-10、IFN-β含量,降低MDA、NO、IL-6、TNF-α、IFN-γ含量,使肾脏细胞排列顺序更为规则,且对DPPH自由基以及ABTS阳离子自由基具有明显的清除效果,也能有效抵抗由D-半乳糖导致的小鼠肾脏萎缩。同时,HPLC分析发现白茶多酚里面含有隐绿原酸、没食子儿茶素没食子酸酯、表儿茶素、芦丁、山柰苷、对香豆酸6种活性物质。总之,白茶多酚具有较好的抗氧化活性,能有效改善由D-半乳糖诱导的小鼠肾损伤,其药理价值可进一步深入研究。

关键词： 白茶多酚; 体外抗氧化活性; D-半乳糖; 肾损伤; 高效液相色谱

本文引用格式

李冲 , 杨悦 , 骞宇 , 赵欣 . 白茶多酚对D-半乳糖致小鼠肾损伤模型的改善作用[J]. 食品与发酵工业, 2022 , 48(4) : 226 -234 . DOI: 10.13995/j.cnki.11-1802/ts.026592

Abstract

To investigate whether white tea polyphenols can ameliorate kidney injury in mice, the kidney damaged mice induced by D- galactose model was established, then treated with different concentrations [250, 500 mg/(kg·d)] of white tea polyphenols lavage mouse model for 42 d. The contents of GSH-Px, CAT, T-AOC, NO, MDA and cytokines IL-6, IL-10, TNF-α, IFN-β and IFN-γ were measured in the kidney of mice at the end of the 6th week. Histology (HE staining) was used to analyze the pathological changes of kidney sections. Antioxidant test in vitro was used to investigate the removal ability of polyphenols to DPPH and ABTS. The renal organ coefficient were also been analyzed. In addition, the HPLC were used to analyze bioactive components of white tea polyphenols. The results showed that polyphenols of white tea (P<0.05) significantly improved the contents of CAT, GSH-PX, T-AOC, IL-10 and IFN-β and reduced the contents of MDA, NO, IL-6, TNF-α and IFN-γ. It also made the sequence of renal cells more regular. Moreover, its apparent effect on scavenged DPPH and ABTS free radicals, and could effectively resist the renal atrophy caused by D-galactose in mice. Furthermore, HPLC analysis showed that polyphenols of white tea contained six active substances which were cryptochlorogenic acid, epigallocatechin gallate, l-epicatechin, rutin, kaempferitrin and p-coumaric acid. In conclusion, polyphenols in white tea have a good antioxidant activity and can effectively improve kidney injury induced by D-galactose in mice, and their pharmacological value could be further studied.

Key words： white tea polyphenols; antioxidant activity in vitro; D-galactose; kindey injury; high performance liquid chromatography

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