建立了柠檬苦素类化合物的QuEChERS-HPLC检测方法,同时测定了不同品种柚类果实中柠檬苦素、诺米林、黄柏酮3种柠檬苦素类化合物含量。样品经乙腈均质超声提取,离心后取上清液,用QuEChERS净化管净化,通过CAPCELL PAK C18 MG 色谱柱(250 mm×4.6 mm,5 μm)梯度洗脱分离,检测波长为210 nm,二极管阵列检测器。结果表明,方法在0.10~200 μg/mL线性良好,相关系数大于0.999,平均回收率为99.0%~100.3%,相对标准偏差为1.0%~2.1%。柚类果实不同品种以及不同果实组织部位中3种柠檬苦素类化合物含量差异显著。该研究可为柠檬苦素类化合物在食品和医药等领域的利用研究提供参考。
The QuEChERS purification-high performance liquid chromatography detection method of limonins was established, and the contents of limonoid, nomilin and corkone in different varieties of pomelo fruits were determined at the same time. The sample was homogenized and ultrasonically extracted with acetonitrile. The supernatant was taken after centrifugation and purified with QuEChERS tube. The chromatographic convention was performed on CAPCELL PAK C18 MG column (250 mm×4.6 mm, 5 μm), and the detection wavelength was set at 210 nm in a diode array detector. The results showed that the linearity was good in the concentration range of 0.1-200 μg/mL, and the correlation coefficient was greater than 0.999. The average recovery was 99.0%-100.3% and the relative standard deviation was 1.0%-2.1%. There were significant differences in the content of three limonoid compounds in different varieties of pomelo fruits and different tissues of fruit. This research provides a reference for the use of limonin compounds in food and medicine industries.
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