以磷脂及玉米醇溶蛋白为载体材料,采用“两步法”构建了脂质包衣的姜黄素纳米负载体系。首先利用溶剂共沉淀法制备姜黄素/玉米醇溶蛋白纳米粒,然后将脂质体与姜黄素/玉米醇溶蛋白纳米粒共同挤压过膜以制备脂质包衣的姜黄素纳米粒。制得的纳米粒粒径较小(90 nm),粒径分布(polydispersity index,PDI=0.234)均匀,同时具备较高的包封率(95.3%)和载药量(17.8%)。随后研究了不同姜黄素纳米粒在不同pH、光照、高温条件下的稳定性、抗氧化活性及模拟胃肠道环境中的释放行为。稳定性实验表明,相较于未包裹的自由姜黄素及未包衣的姜黄素/玉米醇溶蛋白纳米粒,经脂质包衣后,颗粒具备更好的水分散性、光热稳定性以及抗氧化性,同时体外释放实验也表明,脂质包衣的纳米粒能够更有效地阻止姜黄素在胃部的泄露,提高姜黄素口服递送至小肠的效率,且在小肠部位缓慢释放。由此说明,脂质包衣的玉米醇溶蛋白纳米递送体系能够显著提高姜黄素的水分散性、理化稳定性及口服递送效率,扩展了姜黄素在食品工业及生物医药领域的应用。
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