研究报告

基于营养元素的茎瘤芥主成分分析和产地溯源

  • 江波 ,
  • 黄建华
展开
  • 1(长江师范学院 现代农业与生物工程学院,重庆,408100)
    2(湖南中医药研究院 中医研究所,湖南 长沙,410013)
硕士,副教授(黄建华研究员为通信作者,E-mail:jhhuang85@163.com)

收稿日期: 2021-05-02

  修回日期: 2021-06-25

  网络出版日期: 2022-04-25

基金资助

国家自然科学基金项目(81603400);重庆市自然科学基金项目(cstc2018jcyjAx0594)

Principal component analysis and geographical origin traceability of tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) base on nutrient elements

  • JIANG Bo ,
  • HUANG Jianhua
Expand
  • 1(School of Advanced Agriculture and Bioengineering, Yangtze Normal University, Chongqing 408100, China)
    2(Institute of Chinese Materia Medica, Hunan Academy of Traditional Chinese Medicine, Changsha 410013, China)

Received date: 2021-05-02

  Revised date: 2021-06-25

  Online published: 2022-04-25

摘要

探寻茎瘤芥中营养元素含量特征规律及其与产地的关系。通过微波消解系统消解茎瘤芥样品,采用电感耦合等离子体发射光谱分析消解液中的常量营养元素(Mg、P、S、Ca、K)和微量营养元素(B、Mo、Mn、Fe、Cu、Zn、Sr),测量数据经标准化处理后,利用主成分分析(principal component analysis,PCA)提取主因子识别茎瘤芥的特征元素,利用聚类分析(cluster analysis,CA)构建判别模型对茎瘤芥产地进行溯源。结果表明,PCA提取的2个主因子累积方差贡献率达85.971%,能体现原始数据的主要信息,因子分析识别出Mg、P、S、K、B、Mn、Zn、Cu为茎瘤芥的特征元素,CA将16种茎瘤芥样品聚为三大类,不同样品中营养元素含量具有明显地域分布特征,可用于茎瘤芥产地的判别。该研究可为茎瘤芥的品质评价和产地溯源提供科学理论依据。

本文引用格式

江波 , 黄建华 . 基于营养元素的茎瘤芥主成分分析和产地溯源[J]. 食品与发酵工业, 2022 , 48(6) : 46 -51 . DOI: 10.13995/j.cnki.11-1802/ts.027925

Abstract

The aim of this study was to explore the content characteristics of nutrient elements in tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) and establish the relationship between nutrient elements and producing area. Tumorous stem mustard samples were digested by microwave system, and the major nutrient elements (Mg, P, S, Ca, K) and trace nutrient elements (B, Mo, Mn, Fe, Cu, Zn, Sr) were simultaneously analyzed in the sample solution by inductively coupled plasma optical emission spectrometry (ICP-OES). After the measurement data were standardized, the principal component analysis was used to extract the principal factors to identify the characteristic elements of tumorous stem mustard. Cluster analysis was used to establish a discriminant model to trace the origin of tumorous stem mustard in different regions. The results showed that the cumulative variance contribution rate of the five principal components extracted by principal component analysis was 85.971%. Factor analysis identified Mg, P, S, K, B, Mn, Zn, and Cu as the characteristic elements of tumorous stem mustard. Cluster analysis showed that 16 tumorous stem mustard samples could be classified into three groups. The contents of nutrient elements in different samples have obvious regional distribution characteristics, which can be used for the identification of the origin of tumorous stem mustard. This research could provide a scientific theoretical basis for the quality evaluation and origin traceability of tumorous stem mustard.

参考文献

[1] 孙勃, 夏雪, 辜金花, 等.笋子芥和茎瘤芥生物活性物质与抗氧化能力分析[J].核农学报, 2016, 30(3):485-492.
SUN B, XIA X, GU J H, et al.Analysis of bioactive compounds and antioxidant capacities in Brassica juncea var.Crassicauiis and Brassica juncea var.tumida[J].Journal of Nuclear Agricultural Sciences, 2016, 30(3):485-492.
[2] LI W F, YANG H Y, LI C M, et al.Chemical composition, antioxidant activity and antitumor activity of tumorous stem mustard leaf and stem extracts[J].CyTA-Journal of Food, 2019, 17(1):272-279.
[3] XIE Q L, HU Z L, ZHANG Y J, et al.Accumulation and molecular regulation of anthocyanin in purple tumorous stem mustard (Brassica juncea var.tumida Tsen et Lee)[J].Journal of Agricultural and Food Chemistry, 2014, 62(31):7 813-7 821.
[4] XIE Q L, YAN F, HU Z L, et al.Accumulation of anthocyanin and its associated gene expression in purple tumorous stem mustard (Brassica juncea var. tumida Tsen et Lee) sprouts when exposed to light, dark, sugar and methyl jasmonate[J].Journal of Agricultural and Food Chemistry, 2019, 67(3):856-866.
[5] SHI H, WANG L L, SUN L T, et al.Cell division and endoreduplication play important roles in stem swelling of tuber mustard (Brassica juncea Coss.var.tumida Tsen et Lee)[J].Plant Biology, 2012, 14(6):956-963.
[6] FANG P, CHEN F B, YAO Q L, et al.Analysis of genetic diversity in the tuber mustard (Brassica juncea var. tumida Tsen et Lee) in the Yangtze River Basin of China[J].Genetic Resources and Crop Evolution, 2013, 60:129-143.
[7] 赵亚南, 刘玉红, 唐振亚, 等.不同养分配比对茎瘤芥产量和营养品质的影响[J].西南大学学报(自然科学版), 2013, 35(1):49-52.
ZHAO Y N, LIU Y H, TANG Z Y, et al.Effects of different fertilizer ratios on the yield and nutrition quality of tumorous stem mustard[J].Journal of Southwest University (Natural Science), 2013, 35(1):49-52.
[8] 吴振, 李红, 杨勇, 等.基于无机元素的花椒产地溯源和品种聚类分析[J].食品科学, 2019, 40(16):213-219.
WU Z, LI H, YANG Y, et al.Geographical origin traceability and varietal classification of Zanthoxylum based on mineral profile[J].Food Science, 2019, 40(16):213-219.
[9] 申丽娟, 丁恩俊, 谢德体, 等.电感耦合等离子体原子发射光谱法测定不同产地山银花金属元素主成分及其聚类分析[J].食品科学, 2014, 35(2):173-176.
SHEN L J, DING E J, XIE D T, et al.Principal component analysis and cluster analysis of metal elements in Flos lonicerae from different areas using inductively coupled plasma-atomic emission spectrometry[J].Food Science, 2014, 35(2):173-176.
[10] 廖享, 高晶, 符继红, 等.ICP-AES法对新疆薰衣草无机元素主成分与聚类分析[J].计算机与应用化学, 2015, 32(7):841-844.
LIAO X, GAO J, FU J H, et al.Xinjiang lavender inorganic element principal components and cluster analysis based on ICP-AES method[J].Computers and Applied Chemistry, 2015, 32(7):841-844.
[11] ŠKRBIĆ B, -DURIŠIĆ-MLADENOVIĆ N, CVEJANOV J.Principal component analysis of trace elements in Serbian wheat[J].Journal of Agricultural and Food Chemistry, 2005, 53(6):2 171-2 175.
[12] KARA D.Evaluation of trace metal concentrations in some herbs and herbal teas by principal component analysis[J].Food Chemistry, 2009, 114(1):347-354.
[13] KARASAKAL A.Determination of major, minor, and toxic elements in tropical fruits by ICP-OES after different microwave acid digestion methods [J].Food Analytical Methods, 2021, 14(2): 344-360.
[14] 庞敏. 明日叶中七种矿物质元素含量测定及营养质量指数分析[J].食品与发酵工业, 2020, 46(20):249-253.
PANG M.Quantitative detection of seven minerals in Ashitaba stem and leaf and the analysis of the index of nutritional quality[J].Food and Fermentation Industries, 2020, 46(20):249-253.
[15] FOSCHI M, D'ARCHIVIO A A, ROSSI L.Geographical discrimination and authentication of lentils (Lens culinaris Medik.) by ICP-OES elemental analysis and chemometrics[J].Food Control, 2020, 118:107438.
[16] 占敏宣, 魏清江, 林雄, 等.PCA再分析采收成熟度对桃溪蜜柚贮藏品质变化模式的影响[J].食品与发酵工业, 2021, 47(9):183-190.
ZHAN M X, WEI Q J, LIN X, et al.Comprehensively analyze the effect of harvest ripening degree maturity on storage quality of Taoxi pomelo based on PCA[J].Food and Fermentation Industries, 2021, 47(9):183-190.
[17] 张孟琴, 徐路, 孙亚真, 等.月季花瓣营养成分评价及主成分和聚类分析[J].食品与发酵工业, 2021, 47(2):274-278.
ZHANG M Q, XU L, SUN Y Z, et al.The evaluation of nutrient component of rose petals and the principal component analysis from different varieties[J].Food and Fermentation Industries, 2021, 47(2):274-278.
[18] 朱周俊, 袁德义, 邹锋, 等.不同锥栗农家种种仁中9 种矿质元素含量的因子分析与聚类分析[J].食品科学, 2019, 40(2):165-170.
ZHU Z J, YUAN D Y, ZOU F, et al.Factor analysis and cluster analysis of contents of 9 mineral elements in seed kernels of Castanea henryi from different varieties[J].Food Science, 2019, 40(2):165-170.
[19] 国家卫生和计划生育委员会,国家食品药品监督管理总局. GB 5009.268—2016 食品安全国家标准 食品中多元素的测定[S].北京:中国标准出版社, 2016.
State Health and Family Planning Commission of the People's Republic of China, State Adiministration for Market Regulation.GB 5009.268—2016 National food safety standard determination of multielement in foods[S].Beijing:Standards Press of China, 2017.
[20] 开建荣, 王彩艳, 赵丹青, 等.红枸杞、黑枸杞和黄枸杞中49种无机元素比较研究[J].食品与发酵工业, 2020, 46(9):152-157.
KAI J R, WANG C Y, ZHAO D Q, et al.Comparative study of 49 inorganic elements in red, black and yellow Lycium barbarum[J].Food and Fermentation Industries, 2020, 46(9):152-157.
[21] BRO R, SMILDE A K.Principal component analysis[J].Analytical Methods, 2014, 6:2 812-2 831.
[22] 张彦聪, 李昀哲, 张军, 等.柠檬椰汁复合果酒的工艺研究及香气特征分析[J].食品与发酵工业, 2021, 47(4):173-181.
ZHANG Y C, LI Y Z, ZHANG J, et al.Fermentation optimization and aroma characteristic of lemon-coconut compound fruit wine[J].Food and Fermentation Industries, 2021, 47(4):173-181.
文章导航

/