该试验探讨了从健康儿童肠道分离的干酪乳杆菌对便秘小鼠肠道粪便菌群组成的影响。选择体重为(20±2)g的小鼠72只,随机分为6组,分别为正常对照组、模型对照组、阳性药物对照组、干酪乳杆菌低剂量组、中剂量组和高剂量组。用复方地芬诺酯灌胃小鼠,建立便秘模型。正常对照组小鼠灌胃生理盐水,模型对照组小鼠灌胃复方地芬诺酯和生理盐水,阳性对照组灌胃复方地芬诺酯和嗜热链球菌,其余各组便秘小鼠灌胃复方地芬诺酯和相应浓度的干酪乳杆菌。灌胃3 d后,采集小鼠肠道内的粪便,用于分析菌群组成。结果表明,与正常对照组相比,便秘小鼠肠道菌群中乳杆菌属、另枝菌属丰度有不同程度的降低,而普雷沃氏菌属、norank_f_Muribaculaceae丰度升高。干酪乳杆菌干预3 d,优势菌群为norank_f_Muribaculaceae、乳杆菌属、拟普雷沃菌属、嗜冷杆菌属。与模型对照组相比,干酪乳杆菌组小鼠肠道菌群中普雷沃氏菌属、norank_f_Muribaculaceae丰度降低,另枝菌属丰度升高;干酪乳杆菌中剂量组、干酪乳杆菌高剂量组中乳杆菌属丰度升高。提示干酪乳杆菌可通过抑制条件致病菌普雷沃氏菌属的增殖,促进肠道中乳杆菌属、另枝菌属的生长,从而改善肠道菌群,达到缓解便秘的作用。
The impact of different Lactobacillus casei doses on the intestinal microbiota in mice was evaluated in this experiment. Seventy-two mice weighing (20±2) g were selected and randomly divided into six groups: normal control group, model control group, positive drug control group, L. casei low-dose group, medium-dose group and high-dose group, respectively. Mice were fed with compound diphenoxylate to establish a constipation model. Mice in the normal control group were fed with saline, and mice in the model control group were fed with compound difenoxil and saline, meanwhile, mice in the positive control group were fed with compound difenoxil and Streptococcus thermophilus, and mice in the remaining groups with constipation were fed with compound difenoxil and corresponding concentrations of L. casei. After 3 days of gavage, feces were collected from the intestinal tract of the mice for analysis of flora composition. The results showed that the abundance of Lactobacillus and Alistipes in the intestinal flora of constipated mice was reduced to different degrees, while the abundance of Prevotella and norank_f_Muribaculaceae was increased compared with the normal control group. After 3 days of Lactobacillus casei intervention, the dominant flora were norank_f_Muribaculaceae, Lactobacillus, Prevotella and Cryobacillus. Compared with the model control group, the abundance of Prevotella and norank_f_Muribaculaceae in the intestinal flora of mice in L. casei groups decreased, and the abundance of Alistipes increased; the abundance of Lactobacillus in the L. casei medium-dose group and high-dose group increased. It is suggested that L. casei can improve the intestinal flora and relieve constipation by inhibiting the growth of Prevotella and promoting the growth of Lactobacillus and Alistipes in the intestinal tract.
[1] POUILLART P R, DÉPEINT F, ABDELNOUR A, et al.Nutriose, a prebiotic low-digestible carbohydrate, stimulates gut mucosal immunity and prevents TNBS-induced colitis in piglets[J].Inflammatory Bowel Diseases, 2009, 16(5):783-794.
[2] JIANG P P, SANGILD P T.Intestinal proteomics in pig models of necrotising enterocolitis, short bowel syndrome and intrauterine growth restriction[J].Proteomics Clinical Applications, 2014, 8(9-10):700-714.
[3] GONZALEZ L M, MOESER A J, BLIKSLAGER A T.Porcine models of digestive disease:The future of large animal translational research[J].Translational Research, 2015, 166(1):12-27.
[4] BHARUCHA A E, PEMBERTON J H, LOCKE G R L.American Gastroenterological Association technical review on constipation[J].Gastroenterology, 2013, 144(1):218-238.
[5] SHARMA N, DOERNER K C, ALOK P C, et al.Skatole remediation potential of Rhodopseudomonas palustris WKU-KDNS3 isolated from an animal waste lagoon[J].Letters in Applied Microbiology, 2015, 60(3):298-306.
[6] KHAN I, ULLAH N, ZHA L J, et al.Alteration of gut microbiota in inflammatory bowel disease (IBD):Cause or consequence? IBD treatment targeting the gut microbiome[J].Pathogens (Basel, Switzerland), 2019, 8(3):126-153.
[7] DELZENNE N M, KNUDSEN C, BEAUMONT M, et al.Contribution of the gut microbiota to the regulation of host metabolism and energy balance:A focus on the gut-liver axis[J].The Proceedings of the Nutrition Society, 2019, 78(3):319-328.
[8] ZHAO Y, ZHANG X.Interactions of tea polyphenols with intestinal microbiota and their implication for anti-obesity[J].Journal of the Science of Food and Agriculture, 2020, 100(3):897-903.
[9] FORD A C, LACY B E, TALLEY N J.Irritable bowel syndrome[J].The New England Journal of Medicine, 2017, 376(26):2 566-2 578.
[10] CHASSARD C, DAPOIGNY M, SCOTT K P, et al.Functional dysbiosis within the gut microbiota of patients with constipated-irritable bowel syndrome[J].Alimentary Pharmacology & Therapeutics, 2012, 35(7):828-838.
[11] WICHMANN A, ALLAHYAR A, GREINER T U, et al.Microbial modulation of energy availability in the colon regulates intestinal transit.[J].Cell Host & Microbe, 2013, 14(5):582-590.
[12] ZHANG Z, RAN C, DING Q W, et al.Ability of prebiotic polysaccharides to activate a HIF1α-antimicrobial peptide axis determines liver injury risk in zebrafish[J].Communications Biology, 2019, 2:274.
[13] NOGUERA A, CENTENO C, LIBRADA S, et al.Clinical use of oral laxatives in palliative care services in Spain[J].Supportive Care in Cancer, 2010, 18(11):1 491-1 494.
[14] 冯丽莉, 王华, 王世杰, 等.即食型乳酸菌菌粉改善肠道健康的研究[J].食品研究与开发, 2017, 38(9):34-37.
FENG L L, WANG H, WANG S J, et al.The study of the improvement of intestinal health by instant type Lactobacillus powder[J].Food Research and Development, 2017, 38(9):34-37.
[15] 田颖, 戴倩倩, 时明慧, 等.双歧杆菌BB12对便秘大鼠的通便作用及对胃肠激素的调节[J].食品科学, 2016, 37(13):204-208.
TIAN Y, DAI Q Q, SHI M H, et al.Bifidobacterium BB12 promotes defecation and regulates gastrointestinal hormones in constipated rats[J].Food Science, 2016, 37(13):204-208.
[16] 易若琨, 骞宇, 王强, 等.植物乳杆菌YS-1对活性炭诱导小鼠便秘的预防作用[J].食品科学, 2017, 38(17):238-243.
YI R K, QIAN Y, WANG Q, et al.Preventive effect of Lactobacillus plantarum YS-1 on activated carbon-induced constipation in mice[J].Food Science, 2017, 38(17):238-243.
[17] 陆文伟, 胡文兵, 曹文金, 等.干酪乳杆菌LC01对小鼠肠道菌群和肠道转运调节作用的剂量效应[J].食品科学, 2019, 40(15):211-217.
LU W W, HU W B, CAO W J, et al.Dose-dependent modulation of intestinal microbiota and peristalsis in mice by Lactobacillus casei LC01[J].Food Science, 2019, 40(15):211-217.
[18] 靳志敏, 张宏博, 刘夏炜, 等.不同剂量植物乳杆菌对小鼠肠道菌群的影响[J].食品工业科技, 2014, 35(24):342-345.
JIN Z M, ZHANG H B, LIU X W, et al.Effects of supplementation of Lactobacillus plantarum with different dosage on fecal microbiota[J].Science and Technology of Food Industry, 2014, 35(24):342-345.
[19] 张董燕, 季海峰, 刘辉,等.卷曲乳杆菌对生长猪生长性能、粪便菌群和短链脂肪酸组成以及血清长链脂肪酸组成的影响[J].动物营养学报, 2019, 31(4):1 564-1 573.
ZHANG D Y, JI H F, LIU H, et al.Effects of Lactobacillus crispatus on growth performance, fecal microbiota and short chain fatty acid composition and serum long chain fatty acid composition of growing pigs[J].Chinese Journal of Animal Nutrition, 2019, 31(4):1 564-1 573.
[20] 王琳琳. 双歧杆菌对便秘的影响及其作用机理研究[D].江苏无锡:江南大学, 2017.
WANG L L, Study of the effects and mechanisms of Bifidobacteria on constipation alleviation[D].Wuxi:Jiangnan University, 2017.
[21] DIMIDI E, CHRISTODOULIDES S, SCOTT S M, et al.Mechanisms of action of probiotics and the gastrointestinal microbiota on gut motility and constipation[J].Advances in Nutrition (Bethesda, Md), 2017, 8(3):484-494.
[22] 郑慧伶, 李艳霞, 张翠丽, 等.四种小鼠便秘模型建立方法的比较[J].现代生物医学进展, 2013, 13(28):5 456-5 459.
ZHENG H L, LI Y X, ZHANG C L, et al. Comparison of four methods for constructing the constipation model in mice[J].Progress in Modern Biomedicine, 2013, 13(28):5 456-5 459.
[23] LIU B, YANG L J, CUI Z F, et al.Anti-TNF-α therapy alters the gut microbiota in proteoglycan-induced ankylosing spondylitis in mice[J].MicrobiologyOpen, 2019, 8(12):e927.
[24] YU Q H, ZHAO J J, XU Z K, et al.Inulin from Jerusalem artichoke tubers alleviates hyperlipidemia and increases abundance of Bifidobacteria in the intestines of hyperlipidemic mice[J].Journal of Functional Foods, 2018, 40:187-196.
