为科学配伍肠道健康食品中的益生菌与益生元,在相同的体外培养条件下评价乳双歧杆菌BL-99、婴儿双歧杆菌YLGB-1496、副干酪乳杆菌K56、副干酪乳杆菌ET-22等4株益生菌对12种益生元的利用特点,高通量筛选典型的合生元组合并分析其发酵特性。研究通过微生物菌落自动化工作站测定所有组合的生长速率,初步筛选特征组合,并与市售典型益生菌乳双歧杆菌BB-12和鼠李糖乳杆菌LGG对照,评价特征组合中益生元对益生菌的促增殖及促产酸能力,并通过益生元降解率和代谢产物分析评价其体外发酵特性。以代谢产物作为主要指标,结果显示不同益生元对益生菌的作用效果差异显著,乳双歧杆菌BL-99与乳糖组合的益生效果较好,而婴儿双歧杆菌YLGB-1496、副干酪乳杆菌K56、副干酪乳杆菌ET-22则与低聚半乳糖组合的益生效果较好。该研究通过高通量组合筛选,为益生菌与益生元的组合提供数据支持,为肠道健康食品开发奠定基础。
To scientifically explore probiotic and prebiotic combinations good for intestinal health, the microbial colony automation workstation was used for high-throughput sequencing. The growth rate from four probiotics (Bifidobacterium lactis BL-99, Bifidobacterium infantis YLGB-1496, Lactobacillus paracasei K56 and Lactobacillus paracasei ET-22) and 12 prebiotics were determined. Commercial probiotics, Bifidobacterium lactis BB-12 and Lactobacillus rhamnosus LGG, were used as control. The results showed that B. lactis BL-99 combined with lactose and B. infantis YLGB-1496, L. paracasei K56, L. paracasei ET-22 combined with galactooligosaccharides showed better effect. This research provides data support for the combination of probiotics and prebiotics through high-throughput sequencing, and lays a foundation for intestinal health food development.
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