食品与发酵工业 >

2022 , Vol. 48 >Issue 7: 64 - 69

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029800

研究报告

清香酒醅来源Lactobacillus plantarum SL32-2自溶酶的酶学性质及其底物特异性初探

  • 来欢欢 ,
  • 张凯悦 ,
  • 田青 ,
  • 董晋文 ,
  • 赵微 ,
  • 崔美林 ,
  • 张秀红
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  • 1(山西师范大学 生命科学学院,山西 太原,030031)
    2(山西师范大学 食品科学学院,山西 太原,030031)
硕士研究生(张秀红教授为通信作者,E-mail:cwm_ming@163.com)

收稿日期: 2021-10-23

  修回日期: 2021-12-03

  网络出版日期: 2022-04-27

基金资助

山西省自然科学基金项目(201901D111286)

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Enzymatic properties and substrate specificity of autolysin from Lactobacillus plantarum SL32-2 derived from fermented grains of light-flavor Baijiu

  • LAI Huanhuan ,
  • ZHANG Kaiyue ,
  • TIAN Qing ,
  • DONG Jinwen ,
  • ZHAO Wei ,
  • CUI Meilin ,
  • ZHANG Xiuhong
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  • 1(College of Life Science,Shanxi Normal University,Taiyuan 030031,China)
    2(College of Food Science,Shanxi Normal University,Taiyuan 030031,China)

Received date: 2021-10-23

  Revised date: 2021-12-03

  Online published: 2022-04-27

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摘要

为研究Lactobacillus plantarum SL32-2自溶酶的酶学性质及底物特异性,以该细菌细胞为底物分析其自溶酶的最适温度及最适pH,结合该菌株在有无外加自溶酶、自溶前后还原糖和氨基酸的变化以及对4种底物的敏感性,分析其自溶酶的底物特异性。结果表明,L.plantarum SL32-2在对数早期培养4 h时,自溶度最高;其自溶酶的最适pH为7.5,最适温度为37 ℃;添加自溶酶后,L.plantarum SL32-2的自溶度增加了12.04%、还原糖和氨基酸分别增加了1.05和42.83倍;底物特异性分析发现,L.plantarum SL32-2自溶酶中β-N-乙酰葡萄糖胺酶、酰胺酶、羧肽酶、内肽酶的活性分别为0.003 6、0.002 7、1.126 7和2.098 7 U,表明作用于肽链的酶活性远高于作用于糖链的酶。因此,有望通过添加自溶酶的方式,控制酒醅中乳酸菌的过量生长。

关键词： 乳杆菌; 自溶酶; 酶学性质; 自溶度; 底物特异性

本文引用格式

来欢欢 , 张凯悦 , 田青 , 董晋文 , 赵微 , 崔美林 , 张秀红 . 清香酒醅来源Lactobacillus plantarum SL32-2自溶酶的酶学性质及其底物特异性初探[J]. 食品与发酵工业, 2022 , 48(7) : 64 -69 . DOI: 10.13995/j.cnki.11-1802/ts.029800

Abstract

In order to study the enzymatic properties and substrate specificity of autolysin from Lactobacillus plantarum SL32-2, the optimal temperature and pH of the autolysin were analyzed with its bacterial cell as substrate; substrate specificity were analyzed with the concentration changes of reducing sugar and amino acids before and after autolysis with or without the addition the autolysin, as well as the sensitivity of four different substrates. The results showed that the autolysis rate of L. plantarum SL32-2 reached the highest when it was cultured for 4 h at the beginning of logarithmic growth stage. The optimal pH and temperature of its autolysin were 7.5 and 37 ℃, respectively. After adding autolysins, the autolysis rate of L. plantarum SL32-2 increased by 12.04%, and the concentration of reducing sugar and amino acids in buffers increased by 1.05 times and 42.83 times, respectively. For substrate-specific analysis, the activity of β-N-acetylglucamase, aminase, carboxyl peptidase and endopeptidase was 0.003 6, 0.002 7, 1.126 7 and 2.098 7 U, respectively, indicating that the enzyme activities acting on the peptide chain were higher than that on sugar chain.

Key words： Lactobacillus; autolysin; enzymatic properties; autolysis rate; substrate specificity

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