以鱼皮明胶(fish skin gelatin,FSG)-海藻酸钠(sodium alginate,Alg)为研究对象,通过对不同比例FSG-Alg复合体系稳定性和相互作用力类型的评估,明确复合体系中FSG与Alg的相互作用及其规律。结果表明,pH是影响FSG-Alg复合体系相互作用的主要原因,复合体系在pH 7时共溶,浊度最小;Zeta电位在pH 7时整体电势更高,此时FSG-Alg复合体系最稳定;荧光光谱和等温滴定量热分析结果表明FSG与Alg的相互作用为静电作用,pH 7时,随着海藻酸钠含量增加呈现出荧光猝灭的趋势,并且在FSG∶Alg质量比为1∶2时,复合体系的蛋白与多糖间相互作用最强。冷场扫描电镜图像表明,在pH 7时FSG与Alg复合形成了新的空间构象,Alg改变了FSG的空间结构。该研究为后续FSG-Alg复合体系的深入研究提供了参考依据。
In this research, fish skin gelatin (FSG) and sodium alginate (Alg) were the research objects and the interaction and rules between FSG and Alg in the FSG-Alg composite system were investigated by evaluating the stability and type of interaction under different proportions of FSG and Alg. The results indicated that pH was the main factor affecting the interaction of FSG-Alg composite system. The composite system was co-soluble at pH 7, and the turbidity was the minimum. The Zeta potential was higher at pH 7 with the most stable of FSG-Alg composite system than at other pH value. Fluorescence spectrum and isothermal titration calorimetry analysis showed that the interaction between FSG and Alg was electrostatic. The fluorescence quenching trend was presented with the increase of Alg concentration at pH 7, and the interaction between protein and polysaccharide in the composite system was the strongest when the mixing ratio of FSG and Alg was 1∶2. Cryo-scanning electron microscopy images indicated that FSG and Alg compounded to form a new spatial conformation at pH 7, and the presence of Alg changed the spatial structure of FSG. This research provides a reference for further study of FSG-Alg composite system.
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