食品与发酵工业 >

2022 , Vol. 48 >Issue 8: 239 - 244

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.028456

分析与检测

高效液相色谱法测定鸡蛋中三种姜黄素类化合物

  • 周禹君 ,
  • 公丕学 ,
  • 戴琨 ,
  • 刘桂亮 ,
  • 薛霞 ,
  • 刘艳明
展开
  • (山东省食品药品检验研究院,山东省特殊医学用途配方食品质量控制工程技术研究中心,山东省食品药品安全检测工程技术研究中心,山东 济南,250101)
第一作者:硕士,工程师(公丕学高级工程师和刘艳明研究员为共同通信作者,E-mail:gongpeixue@126.com;msymliu@126.com)

收稿日期: 2021-06-24

  修回日期: 2021-08-02

  网络出版日期: 2022-05-18

基金资助

国家重点研发计划 (2017YFC1601600)

收起

Determination of three curcumin compounds in eggs by high performance liquid chromatography

  • ZHOU Yujun ,
  • GONG Pixue ,
  • DAI Kun ,
  • LIU Guiliang ,
  • XUE Xia ,
  • LIU Yanming
Expand
  • (Shandong Institute for Food and Drug Control, Shandong Research Center of Engineering and Technology for Quality Control of Food for Special Medical, Shandong Research Center of Engineering and Technology for Safety Inspection of Food and Drug, Jinan 250101, China)

Received date: 2021-06-24

  Revised date: 2021-08-02

  Online published: 2022-05-18

Fold

摘要

建立了检测鸡蛋中3种姜黄素类化合物的高效液相色谱-紫外/二极管阵列检测方法。样品经搅打混匀,乙腈分散提取,正己烷除脂,通过Atlantis T3 C18色谱柱分离,以V(0.2%甲酸,体积分数)∶V(乙腈)=1∶1为流动相,流速1.0 mL/min,紫外/二极管阵列检测器检测,检测波长为425 nm,外标法定量。结果表明,3种姜黄素类化合物在0.05~100 μg/mL线性关系良好,线性相关系数均大于0.999 9,方法的加标回收率在85%~101%,精密度在0.34%~4.16%,3种姜黄素类化合物的检出限可达0.5 mg/kg,定量限可达1 mg/kg。该方法灵敏度高、重复性好、结果准确,适用于鸡蛋中3种姜黄素类化合物的分析检测。

关键词： 姜黄素类化合物; 紫外/二极管阵列检测器; 鸡蛋; 高效液相色谱法

本文引用格式

周禹君 , 公丕学 , 戴琨 , 刘桂亮 , 薛霞 , 刘艳明 . 高效液相色谱法测定鸡蛋中三种姜黄素类化合物[J]. 食品与发酵工业, 2022 , 48(8) : 239 -244 . DOI: 10.13995/j.cnki.11-1802/ts.028456

Abstract

A high-performance liquid chromatography method with an ultraviolet detector or photodiode array detector for the detection of three curcumin compounds in eggs was established. The sample was crushed and mixed, decentralized extraction of acetonitrile, greased removal of N-hexane. The separation was performed by Atlantis T3 C18 chromatographic columns, acetonitrile with 0.2% formic acid water (V/V, 1∶1) as mobile phase, flow rate 1.0 mL/min, diode detector detection, detection wavelength 425 nm, external standard legal quantity. It turns out, the linear relationship between the three curcuminoids was in the range of 0.05-100 μg/mL. The linear correlation coefficient was greater than 0.999 9 with a recovery rate of 85%-101%. The degree of precision was between 0.34% and 4.16%, detection limit of the three curcuminoids up to 0.5 mg/kg and quantitative limit up to 1 mg/kg. The method has high sensitivity, good repeatability and accurate results, it is suitable for the analysis and detection of three curcumin compounds in eggs.

Key words： curcumin compounds; ultraviolet detector or photo-diode array detector; egg; high-performance liquid chromatography

参考文献

[1] 秦晓燕, 龚菊梅, 陈卫东.总姜黄素3种单体高纯度同时分离方法[J].安徽中医学院学报, 2012, 31(1):73-77.
QIN X Y, GONG J M, CHEN W D.Simultaneous separation of three high purity monomers from curcuminoids[J].Journal of Anhui Traditional Chinese Medical College, 2012, 31(1):73-77.
[2] 史晶晶, 冯素香, 郝蕊, 等.姜黄提取物中姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素的含量测定[J].中医学报, 2015, 30(6):853-855.
SHI J J, FENG S X, HAO R, et al.Demethoxycurcumin and bisdemethoxycurcumin in Curcuma longa L.extractive[J].China Journal of Chinese Medicine, 2015, 30(6):853-855.
[3] MITO S, WATANABE K, HARIMA M, et al.Curcumin ameliorates cardiac inflammation in rats with autoimmune myocarditis[J].Biological & Pharmaceutical Bulletin, 2011, 34(7):974-979.
[4] WILKEN R, VEENA M S, WANG M B, et al.Curcumin:A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma[J].Molecular Cancer, 2011, 10:12.
[5] VALKO M, LEIBFRITZ D, MONCOL J, et al.Free radicals and antioxidants in normal physiological functions and human disease[J].The International Journal of Biochemistry & Cell Biology, 2007, 39(1):44-84.
[6] 袁鹏, 陈莹, 肖发, 等.姜黄素的生物活性及在食品中的应用[J].食品工业科技, 2012, 33(14):371-375.
YUAN P, CHEN Y, XIAO F, et al.The bioactivities of curcumin and its application in foods[J].Science and Technology of Food Industry, 2012, 33(14):371-375.
[7] 韩刚, 霍文, 李秋影, 等.姜黄素的稳定性研究[J].中成药, 2007, 29(2):291-293.
HAN G, HUO W, LI Q Y, et al.Study on the stability of curcumin[J].Chinese Traditional Patent Medicine, 2007, 29(2):291-293.
[8] 王雪梅, 陈利华, 施文婷.姜黄素类化合物的光稳定性研究[J].安徽大学学报(自然科学版), 2012, 36(3):73-78.
WANG X M, CHEN L H, SHI W T.Study on the photo-stability of curcuminoid[J].Journal of Anhui University (Natural Science Edition), 2012, 36(3):73-78.
[9] 刘永涛, 李乐, 徐春娟, 等.超高效液相色谱同时测定渔用饲料中双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素[J].中国渔业质量与标准, 2016, 6(5):60-64.
LIU Y T, LI L, XU C J, et al.Simultaneous determination of bisdemethoxycurcumin, demethoxycurcumin and curcumin in fishery feed by UPLC[J].Chinese Fishery Quality and Standards, 2016, 6(5):60-64.
[10] 黄燕芬, 洪行球.分光光度法测定总姜黄素的含量及方法学考察[J].浙江中医学院学报, 1999, 23(6):24-25;70.
HUANG Y F, HONG X Q.Measuring the total contents of curcumin with spectrophotometry and methodological observation[J].Journal of Zhejiang College of TCM, 1999, 23(6):24-25;70.
[11] 严建伟, 鲍慰文, 梁炳圻, 等.荧光分光光度法测定姜黄制剂中的姜黄素[J].中国现代应用药学, 1998, 15(2):44-45.
YAN J W, BAO W W, LIANG B Q, et al.Determination of curcumin in Curcuma longa L.of perparation by spectrophotofluorimetry[J].Chinese Journal of Modern Applied Pharmacy, 1998, 15(2):44-45.
[12] JADHAV B K, MAHADIK K R, PARADKAR A R.Development and validation of improved reversed phase-HPLC method for simultaneous determination of curcumin, demethoxycurcumin and bis-demethoxycurcumin[J].Chromatographia, 2007, 65(7-8):483-488.
[13] SIMONOVSKA B, VOVK I, GLAVNIK V, et al.Effects of extraction and high-performance liquid chromatographic conditions on the determination of lutein in spinach[J].Journal of Chromatography A, 2013, 1 276:95-101.
[14] 夏春. 高效液相色谱法测定食品中的姜黄素[J].化学分析计量, 2011, 20(1):54-55.
XIA C.Determination of the curcumin in food by HPLC[J].Chemical Analysis and Meterage, 2011, 20(1):54-55.
[15] 李伟, 肖红斌, 王龙星, 等.高效液相色谱-串联质谱法分析姜黄中微量的姜黄素类化合物[J].色谱, 2009, 27(3):264-269.
LI W, XIAO H B, WANG L X, et al.Analysis of minor curcuminoids in Curcuma longa L.by high performance liquid chromatography-tandem mass spectrometry[J].Chinese Journal of Chromatography, 2009, 27(3):264-269.
[16] VIJAYA SARADHI U V R, LING Y H, WANG J, et al.A liquid chromatography-tandem mass spectrometric method for quantification of curcuminoids in cell medium and mouse plasma[J].Journal of Chromatography B, 2010, 878(30):3 045-3 051.
[17] 吴桂碧. 薄层扫描法测定定姜黄中姜黄素的含量[J].华西药学杂志, 1995,10(3):172-174.
WU G B.Determination of curcumin in Curcuma by TLC-scanning densitometry[J].West China Journal of Pharmaceutcal Sciences, 1995,10(3):172-174.
[18] TANAKA K, KUBA Y, SASAKI T, et al.Quantitation of curcuminoids in Curcuma rhizome by near-infrared spectroscopic analysis[J].Journal of Agricultural and Food Chemistry, 2008, 56(19):8 787-8 792.
[19] 刘保启, 胡孝忠, 王玉春, 等.高效毛细管电泳法测定姜黄中姜黄素类化合物[J].分析测试学报, 2004, 23(1):109-111.
LIU B Q, HU X Z, WANG Y C, et al.HPCE determination of curcumins in Curcuma longa L.[J].Journal of Instrumental Analysis, 2004, 23(1):109-l11.
[20] THONGCHAI W, LIAWRUANGRATH B, LIAWRUANGRATH S.Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay[J].Food Chemistry, 2009, 112(2):494-499.
[21] 程司, 刘智广, 曹云新, 等.姜黄素的化学测定的新方法[J].第四军医大学学报, 2000(2):241-243.
CHENG S K, LIU Z G, CAO Y X, et al.New method in the determination of curcumin by electrochemistry[J].Journal of the Fourth Military Medical University, 2000(2):241-243.
Options
文章导航

/

〈 〉

技术支持：北京玛格泰克科技发展有限公司