降低培养基中的氮源浓度有助于鼠李糖乳杆菌耐受热胁迫和氧化胁迫,但是氮源浓度的降低是否会影响菌株对其他胁迫的耐受尚不清晰。该文通过去除MRS培养基中的蛋白胨,研究低氮源环境下鼠李糖乳杆菌hsryfm 1301的胁迫耐受能力。结果显示,交叉适应和低氮源配合能够进一步提高菌株的热胁迫和氧化胁迫耐受能力。低氮源培养基中,指数期菌体在热胁迫和氧化胁迫下的存活率分别上升了129倍和40倍,而且对酸胁迫、胆盐胁迫、冷冻胁迫和渗透压胁迫的耐受能力未受影响。表明低氮源培养和交叉适应配合将有利于提高菌株在喷雾干燥等造成热胁迫和氧化胁迫的生产过程中的存活率。
The heat and oxidative tolerance of Lactobacillus rhamnosus hsryfm 1301 was strengthened in low nitrogen source environment, but it was unknown whether low nitrogen source would affect the tolerance of L. rhamnosus hsryfm 1301 to other stresses. The influence of nitrogen sources on the stress tolerance of L. rhamnosus was investigated. It was shown that the thermotolerance and aerotolerance of L. rhamnosus hsryfm 1301 were further strengthened by low nitrogen source environment and cross-adaptation. Under low nitrogen source condition, the thermotolerance and aerotolerance of L. rhamnosus hsryfm 1301 increased by 129 and 40 times, respectively, during the exponential phase, while its tolerance to other common stresses such as acid stress, bile salt stress, freezing stress and osmotic stress, was not influenced. It was suggested that low nitrogen source environment and cross-adaptation could benefit the tolerance of L. rhamnosus to heat stress and oxidative stress during production processes such as spray drying.
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