该文以复合改性小米淀粉稳定的Pickering乳液负载β-胡萝卜素,研究了功能性Pickering乳液的pH稳定性、盐离子稳定性、贮存稳定性,通过透析实验、贮存实验、模拟人体胃肠消化实验,研究功能活性物质在复杂环境中的荷载特性和释药特性。结果表明,负载β-胡萝卜素的复合改性小米淀粉Pickering乳液具有良好的pH稳定性、盐离子稳定性和贮存稳定性;β-胡萝卜素在复合改性小米淀粉Pickering乳液的初始负载率为92.92%,相较单一改性辛烯基琥珀酸淀粉(82.44%)显著提高;光照、高温等不良条件会引起负载率下降,15 d后复合改性小米淀粉Pickering乳液中β-胡萝卜素负载率依次为71.71%(4 ℃避光)>62.60%(25 ℃避光)>58.25%(25 ℃光照)>51.97%(37 ℃避光)。透析释放和模拟体外胃肠消化实验结果表明,复合改性小米淀粉稳定的Pickering乳液对β-胡萝卜素有较强的保护作用,有效降低了β-胡萝卜素释放速率,提升了β-胡萝卜素保留率。
Composite modified millet starch prepared by hydrophobic modification of octenyl succinic acid (OSA) and ball milling was used to stabilize Pickering emulsion and loaded with β-carotene. The stability of Pickering emulsion was confirmed at different pH salt and storage conditions. The loading and release characteristics of active substances in a complex environment were evaluated by dialysis experiment, storage experiment and simulated human gastrointestinal digestion experiment. The results showed that composite modified millet starch Pickering emulsion loaded with β-carotene showed good pH, salt ion and storage stability. The initial loading rate of β-carotene in Pickering emulsion of composite modified millet starch was 92.92%, which was significantly higher than that of OSA starch with single modified (82.44%). After 15 days, the loading rate of β-carotene in the Pickering emulsion of composite modified millet starch was as follows: 71.71% (4 ℃, light) >62.60% (25 ℃, light) >58.25% (25 ℃, light) >51.97% (37 ℃, light), which was due to adverse conditions such as light and high temperature. Dialysis release and simulated gastrointestinal digestion in vitro experimental results showed that Pickering emulsion stabilized by compound modified millet starch had a strong protective effect on β-carotene, effectively reduced the releasing rate, and increased the retention rate of β-carotene.
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