食品与发酵工业 >

2022 , Vol. 48 >Issue 11: 30 - 35

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029281

研究报告

外源甘露醇复壮草菇退化菌株的研究

  • 程志虹 ,
  • 王文沛 ,
  • 谭强飞 ,
  • 张凡红 ,
  • 贠建民 ,
  • 赵风云
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  • (甘肃农业大学 食品科学与工程学院,甘肃 兰州,730070)
第一作者:硕士研究生(赵风云副教授为通信作者,E-mail:fyzhao@gsau.edu.cn)

收稿日期: 2021-09-03

  修回日期: 2021-09-27

  网络出版日期: 2022-06-23

基金资助

国家自然科学基金项目(32060708)

收起

Degraded strains of Volvariella volvacea rejuvenated by exogenous mannitol

  • CHENG Zhihong ,
  • WANG Wenpei ,
  • TAN Qiangfei ,
  • ZHANG Fanhong ,
  • YUN Jianmin ,
  • ZHAO Fengyun
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  • (College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, China)

Received date: 2021-09-03

  Revised date: 2021-09-27

  Online published: 2022-06-23

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摘要

甘露醇是一种天然的六碳糖醇,具有调节渗透压、清除活性氧等功能。以同等碳量的6种碳源(海藻糖、甘露醇、蔗糖、果糖、麦芽糖及可溶性淀粉)代替传统马铃薯葡萄糖琼脂培养基中的葡萄糖,对草菇退化菌株进行复壮研究。结果表明,甘露醇、海藻糖、蔗糖均可有效提高退化菌株的气生菌丝密度、菌丝生长速度和生物量,以甘露醇的复壮效果最佳,对其色度培养基(liquid medium supplemented with bromothymol blue and lactose,LBL)脱色能力及基质降解相关酶活力进行进一步测定。结果表明,甘露醇处理组对草菇退化菌株D2、D3的LBL脱色能力显著提高,并且能较大程度恢复滤纸酶、内切葡聚糖酶、漆酶和锰过氧化物酶活力。对退化程度较轻的D1、D2菌株,将碳源更换为甘露醇后,其性状可复壮至原菌株D0的状态。

关键词： 草菇; 甘露醇; 退化菌株; 复壮; 碳源; 纤维素酶

本文引用格式

程志虹 , 王文沛 , 谭强飞 , 张凡红 , 贠建民 , 赵风云 . 外源甘露醇复壮草菇退化菌株的研究[J]. 食品与发酵工业, 2022 , 48(11) : 30 -35 . DOI: 10.13995/j.cnki.11-1802/ts.029281

Abstract

Mannitol is a natural six-carbon sugar alcohol, which has the functions of regulating osmotic pressure and removing reactive oxygen species. In this study, six carbon sources with the same carbon content (trehalose, mannitol, sucrose, fructose, maltose and soluble starch) were used to replace the glucose in potato dextrose agar (PDA), and the rejuvenation of the degraded strains of Volvariella volvacea was carried out. The results showed that: mannitol, trehalose and sucrose effectively increased the aerial mycelium density, mycelial growth rate and biomass of degraded strains. Mannitol had the optimal rejuvenating effect. Therefore, its liquid medium supplemented with bromothymol blue and lactose (LBL) decolorization ability and matrix degradation enzyme activity was further determined. The results showed that the mannitol treatment group significantly improved the LBL decolorization ability of the degraded strains D2 and D3 of V. volvacea. And it obviously restored the activity of filter paper enzyme, endoglucanase, laccase and manganese peroxidase. For the D1 and D2 with less degradation degree, their traits rejuvenated to the state of the original strain D0 after replacing the carbon source with mannitol. This study provided a reference for the research on the rejuvenation of V. volvacea and other degraded strains of edible fungi.

Key words： Volvariella volvacea; mannitol; degenerate strain; rejuvenation; carbon source; cellulase

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