采用微波辅助低共熔溶剂(deep eutectic solvent,DES)提取螺旋藻多糖,并对提取的多糖进行部分分离纯化后,评价其体外抗氧化活性和抗癌活性。对9种不同的DESs组合溶剂提取效果进行了比较,采用三水平四变量Box-Behnken设计和响应面法实验设计确定了DES提取螺旋藻多糖的最佳提取条件。结果表明:氯化胆碱和1, 4-丁二醇以1∶4物质的量比组成的DES体系对多糖的提取效果最佳。当DES含水量29%、提取时间28 min、提取温度81 ℃和固液比20 mg/mL,在此条件下,获得的多糖平均得率为109.80 mg/g。螺旋藻中提取的多糖具有良好的清除DPPH和ABTS阳离子自由基体外抗氧化活性。多糖对人宫颈癌HeLa细胞生长速度有很强的抑制作用。因此,DES作为一种环境友好的溶剂,可以应用于从各种植物材料中提取多糖。
Based on natural deep eutectic solvent (DES), microwave-assisted green extraction was applied for production of polysaccharides from Spirulina platensis. After partial separation and purification of the polysaccharides extracted by DES, antioxidant and anticancer activity in vitro were evaluated. Nine different combinations of deep eutectic solvents were evaluated for extraction of polysaccharides from S. platensis, and the results showed that DES system composed of choline chloride and 1,4-butanediol with molar ratio of 1:4 possessed the optimal extraction efficiency for polysaccharides. The maximum yields of polysaccharides109.80 mg/g was attained at 81 ℃, extracted for 28 min and solid-liquid ratio of 20 mg/mL, with DES water content of 29%. The results revealed that polysaccharides extracted from S. platensis exhibited excellent antioxidant activities in vitro, including DPPH and ABTS radical-scavenging activity. For human cervical cancer HeLa cells, the polysaccharides possessed a strong inhibition effect on the growth rate. Thus, DES, as an environmentally-friendly solvent, can be applied to extract polysaccharides from plant materials.
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