将天然抗氧化剂酚类与白果壳纳米纤维素结合,探究纤维素对不同种类多酚的吸附行为及动力学的影响,并制备一种具有抗氧化功能的纤维素/酚复合颗粒。该研究使用氮气吸附仪对白果壳纳米纤维素进行孔性能表征,研究了其对儿茶素、茶多酚、表没食子儿茶素没食子酸酯、没食子酸和阿魏酸的吸附行为,采用一级和二级动力学模型对吸附曲线进行拟合,以Weber-Morris颗粒间扩散模型描述吸附过程,用X射线光电子能谱分析纤维素吸附多酚前后的表面化学结构变化,以DPPH自由基、ABTS阳离子自由基清除能力为标准,考察复合颗粒的抗氧化能力。实验结果表明,白果壳纳米纤维素的比表面积为246.71 m2/g,孔体积为0.35 cm3/g,选取的5种酚类物质均可以自发吸附到纤维素上,展示出快速—慢速—平衡的吸附过程。其中白果壳纤维素对儿茶素的吸附能力最大,可达3.7 mg/g纤维素。以儿茶素为例,进一步探究离子强度、pH、温度3种环境因素对吸附情况的影响,中性pH和高离子强度有利于纤维素对酚的吸附,而温度变化对吸附作用没有显著影响。纤维素吸附儿茶素后,其表面的化学结构发生变化。吸附儿茶素的纳米纤维素颗粒展现出一定的抗氧化能力,可为纤维素的应用提供更多的可能。
There is a certain spontaneous adsorption behavior between polyphenols and plant cell wall materials, and this adsorption behavior has a positive impact on the bioavailability and processing utilization of polyphenols. This research attempted to combine natural antioxidant polyphenols with ginkgo husk nanocellulose, aiming to explore the adsorption behavior and kinetics of different types of polyphenols by cellulose and to prepare a cellulose/phenol composite particle with antioxidant function. In this experiment, the pore properties of ginkgo shell nanocellulose were characterized by nitrogen adsorption apparatus, and the adsorption behavior of catechins, tea polyphenols, EGCG, gallic acid and ferulic acid on the nanocellulose of the ginkgo shell was studied. The first- and second-order kinetic models were used to fit the adsorption curves, the Weber-Morris interparticle diffusion model was used to describe the adsorption process, the surface chemical structure of cellulose before and after adsorption of polyphenols was analyzed by XPS, and the anti-oxidation ability of the composite particles was investigated based on the DPPH/ABTS+ free radical scavenging ability. The experimental results showed that the specific surface area and pore volume of nanocellulose particles were 246.71 m2/g and 0.35 cm3/g, respectively. The five selected polyphenols could all be adsorbed on cellulose spontaneously, which showing a fast-slow-balanced adsorption process. Among them, the adsorption capacity of ginkgo husk cellulose for catechins was the largest, which reaching 3.7 mg/g cellulose. Taking catechins as an example, the effects of ionic strength, pH and temperature on the adsorption were further investigated. Neutral pH and high ionic strength were beneficial to the adsorption of polyphenols by cellulose, however, temperature changes had no significant effect on the adsorption. After cellulose adsorbs catechins, the chemical structure of its surface changes. The catechin-adsorbed nanocellulose particles exhibit a certain antioxidant capacity, which provides more possibilities for the application of cellulose.
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