Excessive accumulation of oxalate in human body can lead to hyperoxaluria, which could eventually result in the symptom of kidney stone. Degrading excessive oxalate in human body is one of an effective way to prevent or relieve hyperoxaluria. Thirty-six lactic acid bacteria strains were isolated from healthy human gut and fermented milks in our previous study. In this study, the strain with oxalate degradation ability were assessed among these 36 strains. Lactobacillus vaginals 1F-6 was screed out due to its high oxalate degradation activity, which could degrade 83.6% of the oxalate present in culture medium. Based on this, the safety assessments of L. vaginals 1F-6 were performed using the acute toxicological experiments in mouse model. Then, a mouse model of hyperoxaluria was established. The efficiency of oxalate degradation of L. vaginals 1F-6 in vivo was analyzed, which was assessed by measuring the index of urinary oxalate excretion, blood urea nitrogen, creatinine concentration and SOD activity, respectively. The results revealed that there were no differences in body weight and organ index between control group and gavage group, which proved that L. vaginals 1F-6 had no acute toxicity in mice. Besides, L. vaginals 1F-6 could significantly decrease the content of urinary oxalate in mice, reduce the aggregation of calcium oxalate crystal, and relieve the ionic disorder and renal the injury caused by oxalic acid overaccumulation in kidney, which had a positive effect of protection on kidney. These findings provided a foundation to develop the probiotic for preventing kidney stones caused by hyperoxaluria.
[1] COE F L, WORCESTER E M, EVAN A P.Idiopathic hypercalciuria and formation of calcium renal stones”[J].Nature Reviews Nephrology, 2016, 12(9):519-533.
[2] 尹银华, 胡莹, 谢彩英, 等.尿石汤配方颗粒对大鼠草酸钙结石肾脏的保护作用[J].今日药学, 2021, 31(2):119-123;139.
YIN Y H, HU Y, XIE C Y, et al.The protective effect of Niaoshitang formula granulesn rat calcium oxalate stone kidney[J].Pharmacy Today, 2021, 31(2):119-123;139.
[3] 苟晓燕, 游芳凝, 王晓磊, 等.白芍水提物对大鼠草酸钙肾结石形成的影响[J].基因组学与应用生物学, 2020, 39(9):4 365-4 371.
GOU X Y, YOU F N, WANG X L, et al.Effect of an aqueous extract of Radix Paeoniae Alba on calcium oxalate nephrolithiasis in rat[J].Genomics and Applied Biology, 2020, 39(9):4 365-4 371.
[4] WU W Q, YANG B C, OU L L, et al.Urinary stone analysis on 12,846 patients:A report from a single center in China[J].Urolithiasis, 2014, 42(1):39-43.
[5] SIENER R, BADE D J, HESSE A, et al.Dietary hyperoxaluria is not reduced by treatment with lactic acid bacteria[J].Journal of Translational Medicine, 2013, 11:306.
[6] 国立东, 王丽群, 于纯淼, 等.乳酸菌降解草酸盐活性及机制研究进展[J].食品科学, 2018, 39(3):324-329.
GUO L D, WANG L Q, YU C M, et al.Advances in understanding oxalate-degrading activity of lactic acid bacteria and underlying mechanism[J].Food Science, 2018, 39(3):324-329.
[7] TICINESI A, NOUVENNE A, MESCHI T.Gut microbiome and kidney stone disease:Not just an Oxalobacter story[J].Kidney International, 2019, 96(1):25-27.
[8] GOMATHI S, SASIKUMAR P, ANBAZHAGAN K, et al.Screening of indigenous oxalate degrading lactic acid bacteria from human faeces and south Indian fermented foods:Assessment of probiotic potential[J].The Scientific World Journal, 2014, 2014:648059.
[9] MURRU N, BLAIOTTA G, PERUZY M F, et al.Screening of oxalate degrading lactic acid bacteria of food origin[J].Italian Journal of Food Safety, 2017, 6(2):6345.
[10] HOKAMA S, HONMA Y, TOMA C, et al.Oxalate-degrading Enterococcus faecalis[J].Microbiology and Immunology, 2000, 44(4):235-240.
[11] MURPHY C, MURPHY S, O’BRIEN F, et al.Metabolic activity of probiotics:Oxalate degradation[J].Veterinary Microbiology, 2009, 136(1-2):100-107.
[12] AFKARI R, FEIZABADI M M, ANSARI-MOGHADAM A, et al.Simultaneous use of oxalate-degrading bacteria and herbal extract to reduce the urinary oxalate in a rat model:A new strategy[J].International Braz Jurol:Official Journal of the Brazilian Society of Urology, 2019, 45(6):1 249-1 259.
[13] KWAK C, JEONG B C, KU J H, et al.Prevention of nephrolithiasis by Lactobacillus in stone-forming rats:A preliminary study[J].Urological Research, 2006, 34(4):265-270.
[14] 任志华. 草酸降解菌的分离鉴定及其对犬草酸钙尿结石的预防作用与机理研究[D].南京:南京农业大学, 2011.
REN Z H.Isolation and identification of oxalate-degrading bacteria and their preventive effect and mechanism on calcium oxalate urolithiasis in canine[D].Nanjing:Nanjing Agricultural University, 2011.
[15] 何春燕, 黄敏华, 李惠萍.比色法测定啤酒中的草酸含量[J].啤酒科技, 2006(11):13-15.
HE C Y, HUANG M H, LI H P.Determination of oxalic acid in beer by colorimetric method[J].Beer Science and Technology, 2006(11):13-15.
[16] KULLIN B, TANNOCK G W, LOACH D M, et al.A functional analysis of the formyl-coenzyme A (frc) gene from Lactobacillus reuteri 100-23C[J].Journal of Applied Microbiology, 2014, 116(6):1 657-1 667.
[17] 郑安然, 邵佩兰, 张丽芬, 等.红枣色素对小鼠急性毒性作用研究[J].食品工业科技, 2017, 38(9):349-352.
ZHENG A R, SHAO P L, ZHANG L F, et al.Study on acute toxicity of jujube pigment in mice[J].Science and Technology of Food Industry, 2017, 38(9):349-352.
[18] HUANG H S, MA M C, CHEN J, et al.Changes in the oxidant-antioxidant balance in the kidney of rats with nephrolithiasis induced by ethylene glycol[J].The Journal of Urology, 2002, 167(6):2 584-2 593.
[19] KHAN S R.Crystal-induced inflammation of the kidneys:Results from human studies, animal models, and tissue-culture studies[J].Clinical and Experimental Nephrology, 2004, 8(2):75-88.
[20] 贺德华, 张华, 王以兵, 等.汉黄芩素对肾结石大鼠肾损伤修复作用及JNK信号通路的调节作用研究[J].中南药学, 2020, 18(11):1 795-1 799.
HE D H, ZHANG H, WANG Y B, et al.Regulatory effect of wogonin on JNK signal pathway and repair of renal injury in rats with renal calculi[J].Central South Pharmacy, 2020, 18(11):1 795-1 799.
[21] GOMATHI S, SASIKUMAR P, ANBAZHAGAN K, et al.Oral administration of indigenous oxalate degrading lactic acid bacteria and quercetin prevents calcium oxalate stone formation in rats fed with oxalate rich diet[J].Journal of Functional Foods, 2015, 17:43-54.
[22] 李丽, 陈菲, 包春娟, 等.介绍一种改良的Von Kossa钙盐染色法[J].临床与实验病理学杂志, 2021, 37(2):234-235.
LI L, CHEN F, BAO C J, et al.Introduction of an improved Von Kossa calcium salt staining method[J].Chinese Journal of Clinical and Experimental Pathology, 2021, 37(2):234-235.
