食品与发酵工业 >

2022 , Vol. 48 >Issue 13: 108 - 114

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.029849

研究报告

蛋白质高产株小球藻MBFJNU-17的异养培养基优化

  • 周有彩 ,
  • 肖雪花 ,
  • 梁志波 ,
  • 何勇锦 ,
  • 陈必链
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  • 1(福建师范大学 生命科学学院,福建 福州,350117)
    2(工业微生物教育部工程研究中心(福建师范大学),福建 福州,350117)
第一作者：博士研究生(陈必链教授为通信作者,E-mail:chenbil@fjnu.edu.cn)

收稿日期: 2021-10-27

  修回日期: 2021-11-18

  网络出版日期: 2022-08-03

基金资助

国家自然科学基金项目(32172997);“十三五”海洋经济创新发展示范项目(FZHJ04)

收起

Medium optimization for Chlorella sp. MBFJNU-17 heterotrophic cultivation

  • ZHOU Youcai ,
  • XIAO Xuehua ,
  • LIANG Zhibo ,
  • HE Yongjin ,
  • CHEN Bilian
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  • 1(College of Life Sciences, Fujian Normal University, Fuzhou 350117,China)
    2(Engineering Research Center of Industrial Microbiology, Ministry of Education, Fujian Normal University, Fuzhou 350117, China)

Received date: 2021-10-27

  Revised date: 2021-11-18

  Online published: 2022-08-03

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摘要

通过单因素试验筛选出以尿素作为小球藻MBFJNU-17异养培养基的单一氮源。以尿素作为氮源时,分批培养条件下小球藻的最高生物量达到10.85 g/L,蛋白质含量为44.5%,同时培养过程中藻液的pH相对稳定,更有利于藻细胞的生长。利用Plackett-Burman法选取了小球藻异养培养基中对微藻生长影响最显著3个因素。随后通过响应面法优化了3个因素的最佳质量浓度,分别为:葡萄糖 41.53 g/L,尿素4.14 g/L,微量元素母液1.84 mL。优化后小球藻细胞干重从10.85 g/L提升至15.53 g/L,提高了43.1%,同时藻细胞蛋白质含量为52.3%。最后对小球藻蛋白与大豆蛋白在FAO/WHO/UNU模式下比较了必需氨基酸指数(essential amino acids index,EAAI),其中异养小球藻的EAAI为0.724,比大豆蛋白(0.657)更高。综合研究结果显示,通过优化小球藻MBFJNU-17培养基,获得了较高的藻生物量,且细胞蛋白质比大豆蛋白更具营养价值,可开发为动物饲料或人类食品蛋白质的优质原料。

关键词： 尿素; 小球藻; 响应面分析; 蛋白质; 必需氨基酸

本文引用格式

周有彩 , 肖雪花 , 梁志波 , 何勇锦 , 陈必链 . 蛋白质高产株小球藻MBFJNU-17的异养培养基优化[J]. 食品与发酵工业, 2022 , 48(13) : 108 -114 . DOI: 10.13995/j.cnki.11-1802/ts.029849

Abstract

Urea was selected as the single nitrogen source for Chlorella sp. MBFJNU-17 heterotrophic cultivation. The results showed that the maximum biomass and the protein content of Chlorella was 10.85 g/L and 44.5% in batch cultivation using urea as nitrogen source. Meantime, the stable pH of cultures could benefit for the growth of Chlorella treated by urea. The significant factors that affected the growth of Chlorella were screened via Plackett-Burman experiments. Further, the concentrations of glucose, urea and trace elements stock solution were optimized by response surface methodology. And the biomass of Chlorella cultivated in the optimized medium was significantly increased to 15.53 g/L with the protein content of 52.3%. The essential amino acid index (EAAI) of Chlorella sp. MBFJNU-17 protein and soybean protein were compared with FAO/WHO/UNU standard. The results showed that the EAAI of Chlorella was 0.724 which higher than soybean protein (0.657). Taken together, Chlorella sp. MBFJNU-17 attained high density of biomass by the medium optimization, and exhibited more nutritional value than soybean protein, which can develop for animal feed or human food protein.

Key words： urea; Chlorella; response surface methodology; protein; essential amino acid

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