综述与专题评论

色谱技术在肽分离中的应用

  • 张硕 ,
  • 陈炬龙 ,
  • 唐雨欣 ,
  • 蔡宝国
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  • (上海应用技术大学 香料香精化妆品学部,上海,201418)
第一作者:博士,副教授(张硕副教授和蔡宝国工程师为共同通信作者,E-mail:zhangshuo0430@163.com;baoguocai@126.com)

收稿日期: 2021-10-11

  修回日期: 2021-11-08

  网络出版日期: 2022-08-03

基金资助

国家自然科学基金项目(32001835)

Application of chromatography in separation of peptides: A review

  • ZHANG Shuo ,
  • CHEN Julong ,
  • TANG Yuxin ,
  • CAI Baoguo
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  • (School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China)

Received date: 2021-10-11

  Revised date: 2021-11-08

  Online published: 2022-08-03

摘要

肽具有分子质量低、易于吸收、生物活性高和低免疫原性等优点,被广泛应用于食品、医疗和化妆品等领域。色谱技术在肽的分离中扮演着重要角色,为肽的分离纯化和产量提高发挥了关键作用。该文重点阐述了离子交换色谱、分子排阻色谱和反相高效液相色谱3种常用色谱技术在肽分离中的应用,分别从技术原理及特点、应用实例进行论述,并对未来色谱技术在肽分离中的应用提出了展望。

关键词: 色谱技术; ; 分离; 纯化

本文引用格式

张硕 , 陈炬龙 , 唐雨欣 , 蔡宝国 . 色谱技术在肽分离中的应用[J]. 食品与发酵工业, 2022 , 48(13) : 327 -335 . DOI: 10.13995/j.cnki.11-1802/ts.029669

Abstract

Peptides with small molecular weight have the advantages of being absorbed easily, high bioactivity and low immunogenicity which are widely used in food, medical treatment and cosmetic. Chromatography technology plays a crucial role in the separation of peptide and is of significance to the separation and purification of peptides and the improvement of its yield. Consequently, this paper focuses on the elaboration of the application of ion exchange chromatography, size exclusion chromatography and reversed-phase high performance liquid chromatography which are the three commonly chromatographic techniques in the separation of peptides. The principle and characteristics, and application examples of three technologies are elaborated, and prospects are put forward for the separation and application of peptides in the future.

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