考察嗜酸乳杆菌和动物双歧杆菌乳亚种益生菌饮料对甲型副伤寒沙门氏菌诱导的腹泻改善效果,并探究其作用机制。饲喂腹泻模型小鼠益生菌饮料原液,观察益生菌饮料对腹泻改善情况;饲喂正常小鼠沙门氏菌和益生菌饮料混合液,观察小鼠腹泻情况,检测粪便中的乳杆菌和双歧杆菌的数量,探究益生菌的竞争抑制作用;饲喂正常小鼠益生菌饮料以及菌粉,检测粪便及胃肠道组织内的乳杆菌和双歧杆菌的数量,探究益生菌的定植作用。结果显示,饲喂益生菌饮料原液的实验组腹泻模型小鼠1 d后腹泻停止,较对照小组恢复快;饲喂沙门氏菌和益生菌饮料混合液,小鼠没有出现水样便和腹泻,且5 d后的粪便中益生菌数量与对照组相近;小鼠肠道内的乳杆菌和双歧杆菌的数目均随着饲喂益生菌饮料以及菌粉时间的延长呈上升趋势。综上,益生菌饮料基于益生菌可通过竞争抑制、定植肠道等作用,改善由甲型副伤寒沙门氏菌引起的小鼠腹泻症状,对肠道起到保护作用。
To discover the effect of probiotic beverage on relieving diarrhea induced by Salmonella paratyphi A and explore its mechanism, the diarrhea model mice were fed with probiotic beverage fermented by Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis. The diarrhea occurrence of mice was observed, and the count of Lactobacillus acidophilus and B. animalis subsp. lactis in feces were detected. The normal mice fed with the probiotics beverage and powder were intended to explore whether the probiotics have intestinal colonization effect. The count of L. acidophilus and B. animalis subsp. lactis in feces and intestinal tract were detected. The results showed that the diarrhea model mice in the experimental group fed with probiotic beverage stopped diarrhea after 1 d, and recovered faster than those in the control group. No watery stools and diarrhea showed up in mice which were fed with S. paratyphi A and probiotics beverage. The count of probiotics in feces after 5 d was similar to the control group. The amount of Lactobacillus acidophilus and B. animalis subsp. lactis in the intestinal tract of mice increased with the feeding time. The results suggested that by means of probiotic competition inhibition and colonization of the intestinal tract, probiotic beverage can relieve the diarrhea symptoms caused by S. paratyphi A in mice, and protect the intestinal tract as well.
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