该文使用明胶基高内相乳液对辣椒素进行了包埋,研究了乳液性质及其对辣椒素气味掩盖和胃刺激性的缓解作用。结果表明,辣椒素的负载对明胶基高内相乳液的液滴分布及表观黏度没有显著影响。电子鼻结果显示,与直接溶解于大豆油相比,高内相乳液包埋较好地掩盖了辣椒素的气味,且掩盖作用随谷氨酰胺转氨酶交联时间延长而增强。辣椒素在胃中的释放研究结果表明,与溶解在游离大豆油中的辣椒素相比,高内相乳液包埋有效降低了辣椒素在胃中的释放量,谷氨酰胺转氨酶交联进一步增强了包埋对胃黏膜的保护作用。此外,体外静态消化模拟结果表明高内相乳液包埋显著增加了肠消化中辣椒素在胶束相中的保留率。总体而言,高内相乳液包埋可有效降低辣椒素对鼻腔及胃黏膜的刺激并提高辣椒素生物利用率。
Capsaicin has certain functional properties, but its pungent smell and damage to the gastric mucosa limit its application in food. Capsaicin was embedded in gelatin-based HIPEs to study the emulsion properties and its effect on capsaicin odor masking and gastric irritation relief. The results showed that the loading of capsaicin had no significant effect on the droplet distribution and apparent viscosity of gelatin-based HIPEs. The results of the electronic nose showed that compared with the direct dissolution in soybean oil, the HIPEs embedding better concealed the odor of capsaicin, and the concealing effect was enhanced with the prolonged transglutaminase (TG) crosslinking time. The results showed that compared with capsaicin dissolved in free soybean oil, HIPEs embedding effectively reduced the release of capsaicin in the stomach, and TG crosslinking further enhanced the protective effect of embedding. In addition, results showed that HIPEs embedding significantly increased the retention rate of capsaicin in the micellar phase in intestinal digestion in vitro static digestion simulation studies. In general, HIPEs embedding effectively reduced the irritation of capsaicin on the nasal cavity and gastric mucosa, and it increased the bioavailability of capsaicin.
[1] KUNJIAPPAN S, SANKARANARAYANAN M, KARAN K B, et al.Capsaicin-loaded solid lipid nanoparticles:Design, biodistribution, in silico modeling and in vitro cytotoxicity evaluation[J].Nanotechnology, 2021, 32(9):95101.
[2] HAYMAN M, KAM P C A.Capsaicin:A review of its pharmacology and clinical applications[J].Current Anaesthesia & Critical Care, 2008, 19(5-6):338-343.
[3] XIANG C, GAO J, YE H, et al.Development of ovalbumin-pectin nanocomplexes for vitamin D 3 encapsulation:Enhanced storage stability and sustained release in simulated gastrointestinal digestion[J].Food Hydrocolloids, 2020, 106:105926.
[4] DU J, DAI H J, WANG H X, et al.Preparation of high thermal stability gelatin emulsion and its application in 3D printing[J].Food Hydrocolloids, 2021, 113:106536.
[5] 高艳. 复合凝聚法制备辣椒素微胶囊的研究[D].长沙:长沙理工大学, 2012.
GAO Y, Preparation of capsaicin microcapsules by complex coacervation[D].Changsha:Changsha University of Science & Technology, 2012
[6] 李颖慧, 王辉, 杨延杰, 等.应用电子鼻评价加工型辣椒果实辣度的方法[J].中国调味品, 2018, 43(12):146-150.
LI Y H, WANG H, YANG Y J, et al.Application of electronic nose in evaluating the pungency degree of processing-type pepper fruit[J].China Condiment, 2018, 43(12):146-150.
[7] WINUPRASITH T, KHOMEIN P, MITBUMRUNG W, et al.Encapsulation of vitamin D 3 in pickering emulsions stabilized by nanofibrillated mangosteen cellulose:Impact on in vitro digestion and bioaccessibility[J].Food Hydrocolloids, 2018, 83:153-164.
[8] BELLESI F A, MARTINEZ M J, PIZONES RUIZ-HENESTROSA V M, et al.Comparative behavior of protein or polysaccharide stabilized emulsion under in vitro gastrointestinal conditions[J].Food Hydrocolloids, 2016, 52:47-56.
[9] 古成. 魔芋甘露聚糖稳定姜黄素纳米乳状液及其缓释消化研究[D].哈尔滨:哈尔滨工业大学, 2019.
GU C.Using konjac glucomannan stabilize curcumin nanoemulsion and slow down its nutrient release during in vitro digestion[D].Harbin:Harbin Institute of Technology, 2019.
[10] FENG T, ZHUANG H N, YE R, et al.Analysis of volatile compounds of Mesona Blumes gum/rice extrudates via GC-MS and electronic nose[J].Sensors & Actuators:B.Chemical, 2011, 160(1):964-973.
[11] 黄鹤, 耿丽晶, 陈博, 等.基于电子鼻对不同发酵阶段蟹酱加热前后特征风味的分析[J].食品工业科技, 2018,39(9):239-242;251.
HUANG H, GENG L J, CHEN B, et al.Analysis of flavor characteristics of crab paste before and after heated in different fermentation stages based on electronic nose[J].Science and Technology of Food Industry, 2018, 39(9):239-242;251.
[12] 刘晔, 葛丽琴, 王远兴.庐山云雾茶挥发性成分主成分分析及产地判别[J].食品科学, 2017, 38(24):60-67.
LIU Y, GE L Q, WANG Y X.Analysis of volatile compounds and geographical origin discrimination of lu mountain clouds-mist tea by principal components analysis[J].Food Science, 2017, 38(24):60-67.
[13] HAN J, ZHANG S, LIU X, et al.Fabrication of capsaicin emulsions:Improving the stability of the system and relieving the irritation to the gastrointestinal tract of rats[J].Journal of the Science of Food and Agriculture, 2020, 100(1):129-138.
[14] LU M W, CAO Y, HO C T, et al.Development of organogel-derived capsaicin nanoemulsion with improved bioaccessibility and reduced gastric mucosa irritation[J].Journal of Agricultural and Food Chemistry, 2016, 64(23):4 735-4 741.
[15] SAMS L, PAUME J, GIALLO J, et al.Relevant pH and lipase for in vitro models of gastric digestion[J].Food & Function, 2016, 7(1):30-45.
[16] GALLIER S, ACTON D, GARG M, et al.Natural and processed milk and oil body emulsions:Bioavailability, bioaccessibility and functionality[J].Food Structure, 2017, 13:13-23.
[17] MCCLEMENTS D J, LI Y.Review of in vitro digestion models for rapid screening of emulsion-based systems[J].Food & Function, 2010, 1(1):32-59.
[18] 王晶晶. 动态体外仿生人胃消化系统的调试和胃排空特性研究[D].苏州:苏州大学, 2019.
WANG J J.Debugging and gastric emptying performance of the dynamic in vitro biomimetic human stomach system[D].Suzhou:Soochow University, 2019.
[19] GUO Q, BELLISSIMO N, ROUSSEAU D.Role of gel structure in controlling in vitro intestinal lipid digestion in whey protein emulsion gels[J].Food Hydrocolloids, 2017, 69:264-272.
[20] 何青. 猪肉肌原纤维蛋白颗粒稳定高内相Pickering乳液的制备及营养输送特性研究[D].锦州:渤海大学, 2020.
HE Q.Preparation and nutrient delivery characteristics of high internal phase pickering emulsion stabled by pork myofibrillar particles[D].Jinzhou:BOHAI University, 2020.
[21] 陈冲, 张宁, 任怡镁, 等.酪蛋白凝胶颗粒对水包油型乳液稳定性及体外消化的影响[J].中国食品学报, 2020, 20(9):30-37.
CHEN C, ZHANG N, REN Y M.et al.The stability and in vitro digestion of oil-in-water emulsions stabilized by casein gel particles[J].Journal of Chinese Institute of Food Science and Technology, 2020, 20(9):30-37.
[22] 沈鹏辉. 基于可控酶解制备大豆蛋白纳米颗粒及其对乳液脂质消化的调控研究[D].广州:华南理工大学, 2020.
SHEN P H.Fabrication of soy protein nanoparticles through controllable enzymatic hydrolysis:Towards modulating lipid digestion in emulsions[D].Guozhou:South China University of Technology, 2020.
[23] CHEN X, ZOU L, LIU W, et al.Potential of excipient emulsions for improving quercetin bioaccessibility and antioxidant activity:An in vitro study.[J].Journal of Agricultural and Food Chemistry, 2016, 64(18):3 653-3 660.
[24] 冯鑫. 基于京尼平交联的食品级明胶Pickering乳液的制备、表征及应用[D].成都:西南大学, 2020.
FENG X.Preparation, characterization and application of the food-grade gelatin pickering emulsion based on genipin cross-linking[D] Chengdu:Southwest University.2020.
[25] TAN H, ZHAO L, TIAN S, et al.Gelatin particle-stabilized high-internal phase emulsions for use in oral delivery systems:Protection effect and in vitro digestion study[J].Journal of Agricultural and Food Chemistry, 2017, 65(4):900-907.