食品与发酵工业 >

2022 , Vol. 48 >Issue 16: 42 - 49

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030048

研究报告

萜烯类化合物基于细胞自噬的初步探究

  • 李一澍 ,
  • 姚逸萍 ,
  • 黄和强 ,
  • 陈杉彬 ,
  • 赵文梅 ,
  • 杨海存 ,
  • 王德良 ,
  • 郝飞克
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  • 1(新疆农业大学 食品科学与药学学院,新疆 乌鲁木齐,830052)
    2(中国食品发酵工业研究院有限公司,北京,100015)
    3(酒类品质与安全国际联合研究中心,北京,100015)
    4(青海互助大麦酒业有限公司,海宁,810500)
硕士研究生(王德良教授级高级工程师和郝飞克高级工程师为共同通信作者,E-mail:wdlpost@163.com;haofeike@outlook.com)

收稿日期: 2021-11-11

  修回日期: 2022-01-04

  网络出版日期: 2022-09-16

基金资助

政府间科技创新合作项目(2021YFF0192000);国家大麦青稞产业技术体系(CARS-05)

收起

Preliminary study of terpenes based on autophagy

  • LI Yishu ,
  • YAO Yiping ,
  • HUANG Heqiang ,
  • CHEN Shanbin ,
  • ZHAO Wenmei ,
  • YANG Haicun ,
  • WANG Deliang ,
  • HAO Feike
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  • 1(College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China)
    2(China National Research Institute of Food and Fermentation Industries Co.Ltd., Beijing 100015, China)
    3(International Joint Research Center of Quality and Safety of Alcoholic Beverages, Beijing 100015, China)
    4(Qinghai Huzhu Barley Wine Co.Ltd., Haining 810500, China)

Received date: 2021-11-11

  Revised date: 2022-01-04

  Online published: 2022-09-16

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摘要

通过采用不同浓度的萜烯类化合物处理细胞,探究青稞酒中所含有的7种萜烯类化合物是否会对细胞自噬产生影响。LO2细胞设立对照组和不同质量浓度萜烯类化合物处理组;采用细胞增殖检测试剂盒8法检测细胞存活率;采用蛋白免疫印迹法检测LO2细胞中微管相关蛋白轻链3(microtubule-associated proteins 1A/1B light chain 3,LC3)的表达情况;采用实时荧光定量(real time quantitative PCR,RT-qPCR)检测LC3和P62蛋白的mRNA表达水平;采用荧光免疫法观察LC3和P62/SQSTM1蛋白的表达水平。结果表明,在营养及饥饿条件下,α-蒎烯处理后的LC3蛋白表达水平和mRNA表达水平呈浓度依赖性上调,P62的 mRNA表达水平降低。其余6种萜烯类化合物:香茅醇、β-石竹烯、月桂烯、罗勒烯、柠檬烯、β-紫罗酮对细胞自噬均无显著影响。在LO2细胞中,萜烯类化合物α-蒎烯或具有促进细胞自噬的作用,其具体作用机理仍需进行深入研究。

关键词： 萜烯类化合物; α-蒎烯; 细胞自噬; LO2细胞

本文引用格式

李一澍 , 姚逸萍 , 黄和强 , 陈杉彬 , 赵文梅 , 杨海存 , 王德良 , 郝飞克 . 萜烯类化合物基于细胞自噬的初步探究[J]. 食品与发酵工业, 2022 , 48(16) : 42 -49 . DOI: 10.13995/j.cnki.11-1802/ts.030048

Abstract

By treating cells with different concentrations of terpene compounds, we investigated whether the seven terpene compounds contained in highland barley wine will affect autophagy. Control group and terpene compound treatment group with different mass concentrations were set up for LO2 cells. CCK-8 method was used to detect cell viability; western blotting was used to detect the expression of microtubule-associated proteins 1A/1B light chain 3 (LC3) in LO2 cells; real-time quantitative polymer chain reaction (RT-qPCR) was used to detect the mRNA expression level of LC3 and P62/SQSTM1 protein; fluorescence immunoassay was used to observe the expression level of LC3 and P62 protein. Results showed that under nutrient and starvation conditions, the LC3 protein expression level and mRNA expression level after α-pinene treatment were up-regulated in a concentration-dependent manner, while the mRNA expression level of P62 decreased. The remaining six terpene compounds, citronellol, β-caryophyllene, myrcene, ocimene, limonene, and β-ionone had no significant effect on autophagy. In LO2 cells, the terpenoid α-pinene may have the effect of promoting autophagy, and its specific mechanism still needs to be studied in depth.

Key words： terpenes; α-pinene; cell autophagy; LO2 cells

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