食品与发酵工业 >

2022 , Vol. 48 >Issue 18: 95 - 100

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030575

研究报告

糯米酒源贝莱斯芽孢杆菌的体外安全性评价

  • 黄芷珊 ,
  • 任红 ,
  • 黄炜健 ,
  • 刘少韵 ,
  • 谢卓珊 ,
  • 钟先锋 ,
  • 黄桂东
展开
  • 1(佛山科学技术学院 食品科学与工程学院,广东 佛山,528231)
    2(广东省传统发酵食品工程技术研究中心,广东 佛山,528231)
    3(广东省食品流通安全控制工程技术研究中心,广东 佛山,528231)
    4(佛山市酿造工程技术研究中心,广东 佛山,528231)
    5(佛山市农业生物制造工程技术研究中心, 广东 佛山,528231)
第一作者:硕士研究生(黄桂东教授为通信作者,E-mail:guidongh78@126.com)

收稿日期: 2021-12-24

  修回日期: 2022-01-24

  网络出版日期: 2022-10-17

基金资助

国家自然科学基金项目(32072198;32101926);广东省自然科学基金项目(2020A1515011444;2019A1515110036;2019A1515110973);广东省教育厅重点领域专项(2020ZDZX1029;2020ZDZX1046);佛山市科技奖培育入库项目(2020001003715);广东省教育厅创新团队项目(2021KCXTD034);广东省研究生教育创新计划资助项目(2019JGXM100);禅城区省以上科学技术奖培育项目(禅经发〔2021〕136)

收起

In vitro safety evaluation of Bacillus velezensis from glutinous rice wine

  • HUANG Zhishan ,
  • REN Hong ,
  • HUANG Weijian ,
  • LIU Shaoyun ,
  • XIE Zhuoshan ,
  • ZHONG Xianfeng ,
  • HUANG Guidong
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  • 1(College of food science and engineering,Foshan university of science and technology, Foshan 528231, China)
    2(Research on traditional fermented food engineering technology in Guangdong province, Foshan 528231, China)
    3(Guangdong food safety control engineering technology research center, Foshan 528231, China)
    4(Foshan brewing engineering technology research center, Foshan 528231, China)
    5(Foshan agricultural biological manufacturing engineering technology research center, Foshan 528231, China)

Received date: 2021-12-24

  Revised date: 2022-01-24

  Online published: 2022-10-17

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摘要

针对前期从糯米酒中分离得到的4株抗逆性强且产蛋白酶特性优良的贝莱斯芽孢杆菌,为充分开发其使用价值,该文对这4株贝莱斯芽孢杆菌分别从耐药性检测、耐药质粒检测、溶血实验、毒力基因PCR检测4个方面展开安全性评价,结果显示,4株菌均对庆大霉素、卡那霉素、链霉素、红霉素、氯霉素、万古霉素敏感;菌株B1、B7、B8对四环素中度敏感,菌株B2对四环素耐药。在耐药质粒检测中,对四环素不敏感的B2中不存在耐药质粒,不具备耐药性通过质粒转移的可能性。在溶血实验中,4株菌皆不溶血。在毒力基因PCR检测中,除菌株B2、B7以外,菌株菌株B1、B8皆检测出携带有溶血性肠毒素hblA基因,但并未检测出携带有编码溶血性肠毒素其他组分的hblC和hblD基因,无法产生溶血素;除菌株B2以外,菌株B1、B7、B8皆检测出携带有非溶血性肠毒素nheC基因,但并未检测出携带编码非溶血性肠毒素其他组分的nheA和nheB基因,无法对蛋白起到抑制作用;4株菌都不具备产生肠毒素和呕吐毒素的可能性。综上,4株菌在耐药性检测、耐药质粒检测、溶血实验、毒力基因PCR检测4个方面皆表现出了良好的安全性,为后续此菌株资源的开发打下坚实的应用基础。

关键词： 贝莱斯芽孢杆菌; 糯米酒; 安全性; 耐药性; 溶血性; 毒力基因

本文引用格式

黄芷珊 , 任红 , 黄炜健 , 刘少韵 , 谢卓珊 , 钟先锋 , 黄桂东 . 糯米酒源贝莱斯芽孢杆菌的体外安全性评价[J]. 食品与发酵工业, 2022 , 48(18) : 95 -100 . DOI: 10.13995/j.cnki.11-1802/ts.030575

Abstract

Four strains of Bacillus velezensis with strong stress tolerance and excellent protease production ability were screened from glutinous rice wine by previous studies of our laboratory. To further explore its application potential, this study evaluated the safety of strains by antibiotic drug sensitivity test, plasmid extraction test, hemolysis test and PCR detection of virulence gene. The results showed that all four strains were susceptible to gentamicin, kanamycin, streptomycin, erythromycin, chloramphenicol and vancomycin, while the strains B1, B7 and B8 were moderately susceptible to tetracycline and the strain B2 was resistant to tetracycline. In the test of drug-resistant plasmids, no resistant plasmid was detected in the strain B2, which did not transfer the resistance through plasmids. Furthermore, the hemolysis test revealed that none of the four strains showed hemolytic activity. In the analysis of virulence factors, the enterotoxin gene hblA was detected in the strains B1 and B8, but the hblC or hblD genes were not found in all four strains. The non-haemolytic enterotoxin gene nheC was detected in the strains B1, B7 and B8, but none of the four strains carried the nheA or nheB genes. Meanwhile, there were no genes of enterotoxin T, cytotoxin K and emetic toxins detected in the four strains. In conclusion, the four strains showed a good safety profile in antibiotic drug sensitivity test, plasmid extraction test, hemolysis test and PCR detection of virulence gene, which provides a solid foundation for the development of these strains.

Key words： Bacillus velezensis; glutinous rice wine; safety; drug resistance; hemolytic; virulence gene

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