基于全基因组测序通过平均核苷酸一致性鉴定生胞梭菌(Clostridium sporogenes)CICC 10385(=CMCC 64941)至种水平。通过形态学和生理生化特征分析获得CICC 10385的基础信息,并采用BGISEQ-500基因测序仪进行全基因组测序分析全基因组平均核苷酸一致性。结果显示,菌株CICC 10385的基因组大小为4 252 276 bp,含有58个contigs,基因组(G+C)mol%为27.9%。该菌16S rRNA基因序列与生胞梭菌(C.sporogenes)、肉毒梭菌(C.botulinum)、孔氏梭菌(C.combesii)和C.tepidum最为相似,平均核苷酸一致性(average nucleotide identity,ANI)分析显示,该菌与生胞梭菌(C.sporogenes)DSM 795T(GCA_001020205.1)ANI值为99.39%,与梭菌属其余菌种ANI值均低于95%,可将CICC 10385鉴定为生胞梭菌(C.sporogenes)。基于全基因组测序的ANI分析是一种准确有效的菌种鉴定方法,为该菌及梭菌属(Clostridium sp.)相关菌种鉴定研究提供了理论依据,为该菌的生物学功能研究奠定了基础。
Clostridium sporogenes CICC 10385 (= CMCC 64941) was identified by average nucleotide identity based on the whole genome sequencing to species level. The basic information of CICC 10385 was obtained by morphological and physiological and biochemical analysis. The whole genome of CICC 10385 was sequenced by BGISEQ-500 and analyzed the average nucleotide identity. The results showed that the genome size of CICC 10385 was 4 252 276 bp, containing 58 contigs, the genomic (G+C) mol% was 27.9%. Based on the analysis for the similarities of 16S rRNA gene sequences, most closely related to C. sporogenes, C. botulinum, C. combesii and C. tepidum. The ANI value of CICC 10385 and C. sporogenes DSM 795T (GCA_001020205.1) was 99.39%, and less than 95% to the other species of Clostridium. CICC 10385 can be identified as C. sporogenes by ANI with the whole genome sequencing technology. ANI analysis based on whole genome sequence should be an accurate and effective method for bacterial identification, which would supply the basis for the identification of Clostridium sp. and for the further study to biological research and application.
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