茉莉酸是一种植物内源激素,在抵抗生物胁迫和非生物胁迫过程中发挥重要作用。该研究以产茉莉酸的伊朗毛色二孢菌CCTCC M2017288为研究对象,比较静态与振荡培养环境下菌株发酵产茉莉酸的参数变化,并利用非靶向代谢组学进行差异分析。结果表明在振荡培养条件下,菌体的生物量大、耗糖速率快但产茉莉酸水平低;同时2种发酵过程中发酵液pH变化、菌体外观形态也不同。通过代谢组学分析,共鉴定出77个差异代谢物;静态与振荡培养比较,差异代谢物下调数量最多的代谢途径是氨基酸代谢;脂质代谢中差异代谢物数量仅有2个,其中茉莉酸上调显著。振荡条件下,菌体摄取的营养物质更多地流向氨基酸代谢和核苷酸代谢,而流向茉莉酸合成的通量相对减少,这在一定程度上解释了振荡条件下茉莉酸产量低的原因,也为认识真菌合成茉莉酸的机制提供了参考。
Jasmonic acid is a plant endogenous hormone that plays an important role in the process of plant resistance to biotic and abiotic stress. The filamentous fungus Lasiodiplodia sp. can secrete jasmonic acid under static culture, while the mechanism of jasmonic acid synthesis is still unclear. In this study, Lasiodiplodia iranensis CCTCC M2017188 was selected as the research object, and L. iranensis was cultured under static and shaking conditions to investigate the influence mechanism of culture environment on jasmonic acid synthesis. The fermentation parameters under two conditions were investigated, and metabolic intermediates were collected for metabolomics analysis.There were significant differences in the metabolic characteristics of L. iranensis under two conditions. The biomass under shaking culture was higher, the substrate consumption rate was faster, while the yield of jasmonic acid was lower, compared with static culture. In addition, the pH fluctuatuion and the morphology of mycelium were different from those in static culture. Through non-targeted metabolomics analysis, a total of 77 different metabolites were identified, 26 of which were enriched in the KEGG metabolic pathway. Comparing static and shaking culture, the metabolic pathway that contains the most down-regulated metabolites was amino acid metabolism, among which dulcitol, S-adenosyl-L-homocysteine, methylmalonic acid, adenine, guanosine and L-tryptophan were down-regulated. And another significantly down-regulated metabolic pathway was nucleotide metabolism. While there are only two different metabolites in lipid metabolism, among which jasmonic acid is significantly up-regulated. Under shaking condition, the nutrients ingested by fungi flew more to amino acid metabolism and nucleotide metabolism, while the flux to jasmonic acid synthesis was relatively reduced. In addition, jasmonic acid synthesis was competitively inhibited by traumatic acid and 3-hexenol metabolic pathways. This explained the reason for the low yield of jasmonic acid under shaking condition to some extent, and is meaningful for understanding the mechanism of jasmonic acid synthesis by fungi.
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