2,3-丁二醇是一种非常重要的平台化学品,广泛用于食品、航空和化工等领域。作为当前研究的热点之一,其合成宿主种类相对偏少,限制了2,3-丁二醇的开发利用。该研究以乙偶姻的显色反应为初筛条件,通过大量筛选,获得了一株产2,3-丁二醇的霍氏肠杆菌WM11,OL636379.1,通过摇瓶实验获得最优的发酵培养基,结合最佳的发酵工艺条件降低副产物积累量,最终在72 h通过补料分批发酵获得65.23 g/L的2,3-丁二醇,转化率达到0.41。最后以绿色可再生的玉米芯水解液为碳源,通过两阶段分批补料发酵,2,3-丁二醇的产量在72 h达到42.92 g/L,生产强度达到0.6 g/(L·h)。
2,3-Butanediol is an essential platform chemical, which is widely used in food, aviation ,and chemical industries. Being one of the current research hotspots, however, very limited species were explored to produce it. Utilizing color reaction of acetoin as the primary screening method, a 2,3-butanediol-producing strain of Enterobacter hormaechei WM11, OL636379.1 was obtained through extensive screening. An optimal fermentation medium was then chosen and the accumulation of by-products was reduced through the fermentation optimization. Finally, a titer of 65.23 g/L and a conversion rate of 0.41 was obtained in 72 h by fed-batch fermentation. In addition, using corn cob hydrolysate as an alternative carbon source, the yield of 2,3-butanediol reached 42.92 g/L in 72 h with a productivity of 0.6 g/(L·h) by two-stage fermentation.
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