以真菌毛韧革菌(Stereum hirsutum) NX-22为研究对象,首次通过液体深层发酵获得了其胞外多糖(Stereum hirsutum polysaccharide, SHPS),通过HPLC、红外光谱、紫外光谱等方法对SHPS结构进行了表征,表明其主体结构为一种β-糖苷键构型的多聚半乳糖。抗氧化活性实验表明,SHPS对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基具有一定的清除效果。该研究还确定了SHPS的最佳发酵培养条件:玉米粉40 g/L,酵母粉3 g/L,MgSO4 0.6 g/L,K2HPO4 1.0 g/L,KH2PO4 1.5 g/L,温度25 ℃,pH为7,发酵周期为5 d,多糖产量为5.964 g/L。该研究为进一步开发毛韧革菌的药用价值,特别是其多糖的制备与应用提供了借鉴。
The exopolysaccharide from Stereum hirsutum NX-22 (SHPS) was obtained by fermentation. The structure of SHPS was characterized by HPLC, infrared spectroscopy, ultraviolet spectroscopy. The main structure of SHPS was a kind of b-glycosidic bond polygalactose. Antioxidant activity tests showed that SHPS had certain scavenging effect on DPPH and hydroxyl free radicals. Maximum SHPS production of 5.964 g/L was obtained at pH 7 and 25℃ for 5 d in medium with 40 g/L corn flour, 3 g/L yeast powder, 0.6 g/L MgSO4, 1.0 g/L K2HPO4, and 1.5 g/L KH2PO4. This study provides a useful reference for further development of medicinal value of S. hirsutum, especially for the preparation and application of its exopolysaccharides.
[1] SUN T, ZHANG Y X, JIANG H, et al.Whole genome sequencing and annotation of Naematelia aurantialba (basidiomycota, edible-medicinal fungi)[J].Journal of Fungi (Basel, Switzerland), 2021, 8(1):6.
[2] 叶彬. 金耳菌种的分子鉴定及出菇实验[D].福州:福建农林大学, 2012.
YE B.Identification and cultivation of golden Tremella(Tremella aurantialba)[D].Fuzhou:Fujian Agriculture and Forestry University, 2012.
[3] 张雯, 瞿伟菁, 张晓玲, 邓云霞, 褚书地.金耳菌丝体多糖降血糖作用研究[J].营养学报, 2004, 26(4):300-303.
ZHANG W, QU W J, ZHANG X L, et al.The anti-hyperglycemic activity of polysaccharides from Tremella aurantialba mycelium[J].Acta Nutrimenta Sinica, 2004, 26(4):300-303.
[4] 张雯, 赵旌旌, 王捷思, 等.金耳菌丝体多糖对实验性2型糖尿病大鼠的降血糖作用研究[J].天然产物研究与开发, 2010, 22(1):49-53.
ZHANG W, ZHAO J J, WANG J S, et al.Hypoglycemic activity of polysaccharides extracted from Tremella aurantialba mycelia in experimental type 2 diabetic rats[J].Natural Product Research and Development, 2010, 22(1):49-53.
[5] 王宣东. 金耳多糖的提取、分离和纯化及抗氧化活性的研究[D].聊城:聊城大学, 2018.
WANG X D.Study on extraction, isolation and purification of polysaccharide from Tremella aurantialba and its antioxidant activity[D].Liaocheng:Liaocheng University, 2018.
[6] 汪虹, 瞿伟菁, 褚书地, 李明娟, 田翠平.金耳菌丝体多糖对小鼠高脂血症的防治作用[J].营养学报, 2002, 24(4):431-432;443.
WANG H, QU W J, CHU S D, et al.Studies on the preventive and therapeutic effects of the polysaccharide of Tremella aurantiabla mycelia on diet-induced hyperlipidemia in mice[J].Acta Nutrimenta Sinica, 2002, 24(4):431-432;443.
[7] 杜秀菊, 潘迎捷, 张劲松, 等.金耳菌丝体醇提物体外抗肿瘤抗病毒活性研究[J].食用菌学报, 2009, 16(4):39-42.
DU X J, PAN Y J, ZHANG J S, et al.Effect of ethanolic extracts of Tremella aurantialba mycelia on the proliferation of selected tumor cells and viruses in vitro[J].Acta Edulis Fungi, 2009, 16(4):39-42.
[8] 李志涛, 彭蕊, 朱冰清, 等.蛹虫草液体发酵培养基的研究[J].农业科技与装备, 2019(2):3-4;7.
LI Z T, PENG R, ZHU B Q, et al.Study on liquid fermentation medium of Cordyceps militaris[J].Agricultural Science ＆ Technology and Equipment, 2019(2):3-4;7.
[9] SUN T, WANG R, SUN D F, et al.High-efficiency production of Tremella aurantialba polysaccharide through basidiospore fermentation[J].Bioresource Technology, 2020, 318:124268.
[10] ZHANG Q, LI Y, ZONG S, et al.Optimization of fermentation of fomes fomentarius extracellular polysaccharide and antioxidation of derivatized polysaccharides[J].Cellular and Molecular Biology, 2020, 66(7):56-65.
[11] YU Y, SHEN M Y, SONG Q Q, et al.Biological activities and pharmaceutical applications of polysaccharide from natural resources:A review[J].Carbohydrate Polymers, 2018, 183:91-101.
[12] ULLAH S, KHALIL A A, SHAUKAT F, et al.Sources, extraction and biomedical properties of polysaccharides[J].Foods (Basel, Switzerland), 2019, 8(8):304.
[13] ALBUQUERQUE P B S, DE OLIVEIRA W F, DOS SANTOS SILVA P M, et al.Skincare application of medicinal plant polysaccharides:A review[J].Carbohydrate Polymers, 2022, 277:118824.
[14] KAUP B A, EHRICH K, PESCHECK M, et al.Microparticle-enhanced cultivation of filamentous microorganisms:Increased chloroperoxidase formation by Caldariomyces fumago as an example[J].Biotechnology and Bioengineering, 2008, 99(3):491-498.
[15] DRIOUCH H, SOMMER B, WITTMANN C.Morphology engineering of Aspergillus niger for improved enzyme production[J].Biotechnology and Bioengineering, 2010, 105(6):1 058-1 068.
[16] 杨静静. 玉米粉对灵芝液体发酵的影响[D].无锡:江南大学, 2015.
YANG J J.Effect of corn powder on Ganoderma lucidum during fermentation[D].Wuxi:Jiangnan University, 2015.
[17] 姜浩, 孙涛, 姚皓昱, 等.食用真菌多糖的研究进展[J].食品工业科技, 2022, 43(12):447-456.
JIANG H, SUN T, YAO H Y, et al.Research progress of edible fungal polysaccharides[J].Science and Technology of Food Industry, 2022, 43(12):447-456.
[18] FENG J, FENG N, TANG Q J, et al.Optimization of Ganoderma lucidum polysaccharides fermentation process for large-scale production[J].Applied Biochemistry and Biotechnology, 2019, 189(3):972-986.
[19] TANG Y J, ZHU L L, LIU R S, et al.Quantitative response of cell growth and Tuber polysaccharides biosynthesis by medicinal mushroom Chinese truffle Tuber sinense to metal ion in culture medium[J].Bioresource Technology, 2008, 99(16):7 606-7 615.
[20] ZHU Z Y, LIU X C, DONG F Y, et al.Influence of fermentation conditions on polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Cordyceps militaris[J].Applied Microbiology and Biotechnology, 2016, 100(9):3 909-3 921.
