研究报告

鲁氏毛霉与乳酸菌复配发酵对咖啡奶酪风味的影响

  • 洪宾 ,
  • 白如彬 ,
  • 任慧玲 ,
  • 姜珊珊 ,
  • 乌日娜 ,
  • 史海粟
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  • 1(沈阳农业大学 食品学院,辽宁 沈阳,110866)
    2(辽宁省食品发酵技术工程研究中心,辽宁 沈阳,110866)
    3(沈阳市微生物发酵技术创新重点实验室,辽宁 沈阳,110866)
硕士研究生(史海粟副教授为通信作者,E-mail:shihaisu@syau.edu.cn)

收稿日期: 2022-01-18

  修回日期: 2022-03-15

  网络出版日期: 2022-12-02

基金资助

国家自然科学基金青年科学基金项目(31801567);辽宁省教育厅一般项目(LSNQN20124);沈阳农业大学博士引进人才项目(880417040)

Effect of Mucor rouxianus and lactic acid bacteria co-fermentation on the flavor of coffee cheese

  • HONG Bin ,
  • BAI Rubin ,
  • REN Huiling ,
  • JIANG Shanshan ,
  • WU Rina ,
  • SHI Haisu
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  • 1(College of Food Science and Technology, Shenyang Agricultural University, Shenyang 110866, China)
    2(Liaoning Food Fermentation Technology Engineering Research Center, Shenyang 110866, China)
    3(Shenyang Key Laboratory of Microbial Fermentation Technology Innovation, Shenyang 110866, China)

Received date: 2022-01-18

  Revised date: 2022-03-15

  Online published: 2022-12-02

摘要

现有的咖啡发酵乳制品菌种单一,口感与风味较为局限。将我国传统腐乳发酵菌鲁氏毛霉与复合乳酸菌(保加利亚乳杆菌∶嗜热链球菌=1∶1)复配,制作一种新型的奶酪发酵剂,并将咖啡粉用于奶酪制作。确定了发酵剂的最优复配质量比为鲁氏毛霉∶复合乳酸菌=2∶1,然后通过单因素试验与响应面法确定奶酪的最佳工艺条件,并以复合乳酸菌发酵咖啡奶酪作为对照组,对咖啡奶酪产品开展品质评价。结果表明,试验组样品中丁酸和月桂酸的含量分别为(124.90±1.05)和(507.30±7.58) mg/100 g,是对照组的10倍;亚油酸含量为(0.90±0.07) mg/100 g,是对照组的8倍。试验所制备的咖啡奶酪产品能够满足人们对奶酪新品的需求。

本文引用格式

洪宾 , 白如彬 , 任慧玲 , 姜珊珊 , 乌日娜 , 史海粟 . 鲁氏毛霉与乳酸菌复配发酵对咖啡奶酪风味的影响[J]. 食品与发酵工业, 2022 , 48(21) : 125 -132 . DOI: 10.13995/j.cnki.11-1802/ts.030734

Abstract

At present, the starters of coffee fermented dairy products are single, resulting in a limited taste and flavor. In this study, co-fermentation of Mucor rouxianus, a type of fungus fermenting traditional sufu, with lactic acid bacteria (LAB) complex (Lactobacillus bulgaricus:Streptococcus thermophilus = 1:1) was performed to make a new type of cheese starter, and the coffee powder were used in cheese making. Firstly, the optimal inoculated ratio of starters was confirmed as M. rouxianus:LAB complex = 2:1. Then, the optimal processing conditions of coffee cheese were determined by single factor experiments and response surface methodology. The quality of coffee cheese was evaluated using the cheese fermented by LAB as the control group. Our results showed that the contents of butyric acid and lauric acid reached (124.90±1.05) and (507.30±7.58) mg/100 g cheese, respectively, which were 10 times higher than those of the control group. The content of linoleic acid reached (0.90±0.07) mg/100 g cheese, which was 8 times higher than that of the control group. The coffee cheese product prepared will help to meet the increasing domestic demand for cheese.

参考文献

[1] 栾滨羽, 史海粟, 李彦博, 等.霉菌奶酪中脂肪酸代谢及其对产品品质影响研究进展[J].食品科学, 2020, 41(9):211-221.
LUAN B Y, SHI H S, LI Y B, et al.A review of free fatty acid catabolism in mold-fermented cheese and its effect on product quality[J].Food Science, 2020, 41(9):211-221.
[2] 李昂, 李键, 孙青, 等.我国霉菌奶酪产品研究现状及分析[J].中国乳品工业, 2017, 45(10):25-27.
LI A, LI J, SUN Q, et al.Research status and analysis of mold cheese products in China[J].China Dairy Industry, 2017, 45(10):25-27.
[3] 唐立伟, 周昱婧, 周芷寒, 等.脂肪替代物在奶酪中的应用研究进展[J].食品安全导刊, 2020(9):168-171.
TANG L W, ZHOU Y J, ZHOU Z H, et al.Research progress in the application of fat substitutes in cheese[J].China Food Safety Magazine, 2020(9):168-171.
[4] 董学文, 张苏苏, 李大宇, 等.脂肪替代物在肉制品中应用研究进展[J].食品安全质量检测学报, 2017, 8(6):1 961-1 966.
DONG X W, ZHANG S S, LI D Y, et al.Research progress of the application of fat substitutes in meat products[J].Journal of Food Safety & Quality, 2017, 8(6):1 961-1 966.
[5] DOĆAGĞAN M, TEKINER ĆAIĞ H.Evaluating starter culture potential of wild Penicillium roqueforti strains from moldy cheeses of artisanal origin[J].Food Bioscience, 2021, 43:101253.
[6] KARIMI K, ZAMANI A.Mucor indicus:Biology and industrial application perspectives:A review[J].Biotechnology Advances, 2013, 31(4):466-481.
[7] WANG L, ZHANG H Y, ZHANG Y, et al.13 C metabolic flux analysis on roles of malate transporter in lipid accumulation of Mucor circinelloides[J].Microbial Cell Factories, 2019, 18(1):154.
[8] DE MELO PEREIRA G V, DE CARVALHO NETO D P, MEDEIROS A B P, et al.Potential of lactic acid bacteria to improve the fermentation and quality of coffee during on-farm processing[J].International Journal of Food Science & Technology, 2016, 51(7):1 689-1 695.
[9] 吴姝宓. 乳清奶酪酥饼的加工工艺及其品质功能特性的研究[D].呼和浩特:内蒙古农业大学, 2018.
WU S M.Study on processing technology and its quality function characteristics of ricotta shortcake[D].Hohhot:Inner Mongolia Agricultural University, 2018.
[10] 李昂, 陈炼红, 李键, 等.雅致放射毛霉添加量对牦牛霉菌奶酪品质的影响[J].食品工业科技, 2017, 38(24):120-125.
LI A, CHEN L H, LI J, et al.Effect of addition amount of Actinomucor elegans on quality of yak mold cheese[J].Science and Technology of Food Industry, 2017, 38(24):120-125.
[11] SUZUKI-IWASHIMA A, MATSUURA H, IWASAWA A, et al.Metabolomics analyses of the combined effects of lactic acid bacteria and Penicillium camemberti on the generation of volatile compounds in model mold-surface-ripened cheeses[J].Journal of Bioscience and Bioengineering, 2020, 129(3):333-347.
[12] 王嫱. 褐色风味发酵乳的研制及主要风味分析[D].沈阳:沈阳农业大学, 2019.
WANG Q.Development of brown flavor fermented milk and main flavor analysis[D].Shenyang:Shenyang Agricultural University, 2019.
[13] 郭琳仪. 复配益生菌及工艺条件对羊乳软质奶酪品质影响研究[D].杨凌:西北农林科技大学, 2019.
GUO L Y.Research on the effect of compound probiotic and technological conditions on the quality of soft goat cheese[D].Yangling:Northwest A & F University, 2019.
[14] 史海粟. δ6脂肪酸脱饱和酶底物选择性研究及其在高山被孢霉中的应用[D].无锡:江南大学, 2016.
SHI H S.Studies on the substrate specificity of delta 6 desaturase and its application in Mortierella alpina[D].Wuxi:Jiangnan University, 2016.
[15] 许女, 王佳丽, 陈旭峰, 等.优良乳酸菌的筛选、鉴定及在酸奶中的应用[J].中国食品学报, 2019, 19(2):98-107.
XU N, WANG J L, CHEN X F, et al.Screening, identification of lactic acid bacteria and application in fermented yogurt[J].Journal of Chinese Institute of Food Science and Technology, 2019, 19(2):98-107.
[16] 李莹莹, 笪久香, 栾广忠, 等.利用3种凝固剂制作新鲜软质大豆干酪的工艺研究[J].食品科学, 2012, 33(16):1-6.
LI Y Y, DA J X, LUAN G Z, et al.Optimization of preparation process for fresh soft soymilk cheese using three different coagulants[J].Food Science, 2012, 33(16):1-6.
[17] 高婉伟, 梁琪, 宋雪梅, 等.成熟条件对牦牛乳硬质干酪理化性质的影响[J].甘肃农业大学学报, 2014, 49(1):140-145.
GAO W W, LIANG Q, SONG X M, et al.Effects of ripening conditions on physical and chemical properties of hard cheese made from yak milk[J].Journal of Gansu Agricultural University, 2014, 49(1):140-145.
[18] 李昌盛. 羊奶干酪加工工艺及成熟特性的研究[D].西安:陕西师范大学, 2009.
LI C S.Processing technology and ripening characteristics of goat cheese[D].Xi’an:Shaanxi Normal University, 2009.
[19] 冶成君, 金乐, 胡勇, 等.牦牛脱脂乳白霉菌软质奶酪后发酵期发酵特性的研究[J].青海畜牧兽医杂志, 2016, 46(2):1-5.
YE C J, JIN L, HU Y, et al.Study on fermentation characteristics of white mold cheese of Yak skim milk during fermentation[J].Chinese Qinghai Journal of Animal and Veterinary Sciences, 2016, 46(2):1-5.
[20] 王巧云. 双霉菌发酵的八公山腐乳品质研究[D].合肥:合肥工业大学, 2019.
WANG Q Y.The quality of Bagongshan sufu by double mold fermentation[D].Hefei:Hefei University of Technology, 2019.
[21] 贺家亮, 李开雄, 陈树兴, 等.成熟温度和时间对半硬质干酪成熟特性影响研究[J].食品科学, 2010, 31(3):123-126.
HE J L, LI K X, CHEN S X, et al.Effects of ripening temperature and time on the quality characteristics of semi-hard cheese[J].Food Science, 2010, 31(3):123-126.
[22] COLLINS Y F, MCSWEENEY P L H, WILKINSON M G.Lipolysis and free fatty acid catabolism in cheese:A review of current knowledge[J].International Dairy Journal, 2003, 13(11):841-866.
[23] ROMEIH E A, MICHAELIDOU A, BILIADERIS C G, et al.Low-fat white-brined cheese made from bovine milk and two commercial fat mimetics:Chemical, physical and sensory attributes[J].International Dairy Journal, 2002, 12(6):525-540.
[24] KILCAWLEY K N, WILKINSON M G, FOX P F.A survey of lipolytic and glycolytic end-products in commercial cheddar enzyme-modified cheese[J].Journal of Dairy Science, 2001, 84(1):66-73.
[25] JI X J, LEDESMA-AMARO R.Microbial lipid biotechnology to produce polyunsaturated fatty acids[J].Trends in Biotechnology, 2020, 38(8):832-834.
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