食品与发酵工业 >

2022 , Vol. 48 >Issue 21: 146 - 151

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030728

研究报告

细菌素CAMT2抗菌纳米纤维膜制备及其特性研究

  • 李啟彬 ,
  • 张雪梅 ,
  • 刘颖 ,
  • 房志家 ,
  • 徐春厚
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  • 1(广东海洋大学 食品科技学院,广东省水产品加工与安全重点实验室,广东省海洋食品工程技术研究中心,广东省海洋生物制品工程实验室,水产品深加工广东普通高等学校重点实验室,广东湛江,524088)
    2(广东海洋大学 滨海农业学院,广东 湛江,524088)
硕士研究生(刘颖教授为通信作者,E-mail:liuying@gdou.edu.cn)

收稿日期: 2022-01-13

  修回日期: 2022-03-01

  网络出版日期: 2022-12-02

基金资助

广东省科技计划项目(2016A020222014,2017A030303079)

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Preparation and characteristics of antibacterial nanofiber membrane containing bacteriocin CAMT2

  • LI Qibin ,
  • ZHANG Xuemei ,
  • LIU Ying ,
  • FANG Zhijia ,
  • XU Chunhou
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  • 1 (College of Food Science and Technology, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Provincial Engineering Technology Research Center of Marine Food, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, Guangdong Ocean University, Zhanjiang 524088, China)
    2(College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China)

Received date: 2022-01-13

  Revised date: 2022-03-01

  Online published: 2022-12-02

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摘要

该研究以解淀粉芽孢杆菌ZJHD3-06的细菌素CAMT2为抗菌活性成分,聚乙烯醇为包埋材料,采用静电纺丝技术制备CAMT2-聚乙烯醇(polyvinyl alcohol,PVA)抗菌纳米纤维膜,并对制得纳米纤维膜的抑菌活性、释放效果、结构以及低温下对常见水产基质中单增李斯特菌的抑制效果进行评价。结果表明,载药量为80 mg/mL的CAMT2-PVA纳米纤维膜抑菌活性保留效果良好,第0.5~2天为突释阶段,第2天进入缓释阶段,第7天后趋于稳定。扫描电镜显示纤维表面光滑平整,无串珠等不规则现象,纤维直径为720 nm;傅立叶红外光谱结果表明,电纺过程PVA对细菌素CAMT2实现了有效的物理包埋;CAMT2-PVA纳米纤维膜在3种典型的水产基质中抑菌效果显著。由此表明,CAMT2-PVA具有良好的潜在应用价值。

关键词： 细菌素; 静电纺丝; 纳米纤维膜; 抑菌; 结构

本文引用格式

李啟彬 , 张雪梅 , 刘颖 , 房志家 , 徐春厚 . 细菌素CAMT2抗菌纳米纤维膜制备及其特性研究[J]. 食品与发酵工业, 2022 , 48(21) : 146 -151 . DOI: 10.13995/j.cnki.11-1802/ts.030728

Abstract

The bacteriocin CAMT2 from Bacillus amyloliquefaciens ZJHD3-06 was used as the antimicrobial active ingredient and polyvinyl alcohol (PVA) was as the encapsulation material. The antibacterial CAMT2—PVA nanofiber membranes were prepared by electrostatic spinning technique, and the inhibition activity, release effect, structure and inhibition effect of the produced nanofiber membranes against Listeria monocytogenes in common aquatic matrices at low temperature were evaluated. The results showed that the CAMT2—PVA nanofibers membranes with a drug loading of 80 mg/mL retained good antibacterial activity, with a quick release phase of 0.5-2 d, a slow-release phase on the second day and a stabilization phase after the seventh day. Scanning electron microscope showed that the surface of the fibers was smooth and flat, without irregularities such as beads, and the diameter of the fibers was 720 nm; the results of Fourier transform infrared showed that the bacteriocin CAMT2 was effectively encapsulated by PVA during the electrospinning process; the inhibition effect of CAMT2—PVA nanofibers membrane was significant in three typical aquatic substrates. This suggests that CAMT2—PVA has good potential applications.

Key words： bacteriocin; electrospinning; nanofiber; antibacterial; structure

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