食品与发酵工业 >

2022 , Vol. 48 >Issue 22: 194 - 200

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.030854

研究报告

基于高通量测序解析浓香型大曲真菌群落结构及其与质量指标的关联性分析

  • 程伟 ,
  • 陈雪峰 ,
  • 陈兴杰 ,
  • 周端 ,
  • 付欢 ,
  • 薛锡佳 ,
  • 鹿静静 ,
  • 代森
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  • 1(陕西科技大学 食品与生物工程学院,陕西 西安,710021)
    2(安徽金种子酒业股份有限公司,安徽 阜阳,236023)
    3(陕西农产品加工技术研究院,陕西 西安,710021)
第一作者：博士研究生,高级工程师(陈雪峰教授为通信作者,E-mail:chenxf@ sust.edu.cn)

收稿日期: 2022-01-25

  修回日期: 2022-02-25

  网络出版日期: 2022-12-20

基金资助

2020年度阜阳市科技计划项目(FK2020-81136);2021年度安徽省“皖北产业创新团队”支持计划项目

收起

Analysis of fungal community structure based on high-throughput sequencing and its correlation with quality indicators of strong-flavor Daqu

  • CHENG Wei ,
  • CHEN Xuefeng ,
  • CHEN Xingjie ,
  • ZHOU Duan ,
  • FU Huan ,
  • XUE Xijia ,
  • LU Jingjing ,
  • DAI Sen
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  • 1(School of Food and Biological Engineering, Shaanxi University of Science & Technology, Xi'an 710021, China)
    2(Jinzhongzi Distillery Co.Ltd., Fuyang 236023, China)
    3(Shaanxi Research Institute of Agricultural Product Processing Technology, Xi'an 710021, China)

Received date: 2022-01-25

  Revised date: 2022-02-25

  Online published: 2022-12-20

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摘要

为明确不同地区浓香型大曲的真菌群落结构及其与质量指标的关联性,采用MiSeq高通量测序技术解析不同地区大曲曲皮与曲心的真菌群落结构,再分别检测其糖化力等7项质量指标,并进行真菌群落及其与质量指标的Spearman相关性分析。结果表明,不同地区浓香型大曲的曲皮共有真菌OTU为43个、曲心共有真菌OTU为47个,曲皮中的根霉(Rhizopus)、根毛霉(Rhizomucor)、布氏白粉菌(Blumeria)和散囊菌(Eurotium)为优势菌属,曲心中的嗜热菌(Thermomyces),散囊菌(Eurotium)和嗜热子囊菌(Thermoascus)为优势菌属,其丰度占比均在10%以上。相关性分析表明,嗜热菌(Thermomyces)与水分呈极显著正相关,被孢霉(Mortierella)与还原糖呈极显著正相关,根霉(Rhizopus)、匍柄霉(Stamphylium)和链格孢属(Alternaria)与糖化力呈显著正相关,罗萨氏菌(Rasamsonia)与酯化力呈显著正相关。该研究对大曲微生物组信息扩充具有一定的参考价值。

关键词： 高通量测序; 浓香型大曲; 真菌菌群多样性; 质量指标; 关联性分析

本文引用格式

程伟 , 陈雪峰 , 陈兴杰 , 周端 , 付欢 , 薛锡佳 , 鹿静静 , 代森 . 基于高通量测序解析浓香型大曲真菌群落结构及其与质量指标的关联性分析[J]. 食品与发酵工业, 2022 , 48(22) : 194 -200 . DOI: 10.13995/j.cnki.11-1802/ts.030854

Abstract

In order to clarify the fungal community structure of strong-flavor Daqu from different regions and its correlation with quality indicators, MiSeq high-throughput sequencing was used to analyze the fungal community structure in surface and inner parts of Daqu from different regions. Saccharification capacity and other quality indicators were detected, and Spearman correlation analysis of fungal community and its quality indicators was taken. The results showed there were 43 fungal operational taxonomic units(OTUs)in common between surface samples, 47 fungal OTUs in inner samples. Rhizopus, Rhizomucor, Blumeria, and Eurotium were the dominant genera in surface samples. While, Thermomyces, Eurotium, and Thermoascus were the dominant genera in inner samples, and their abundances were all account for more than 10%. The correlation analysis showed that there was a significant positive correlation between Thermomyces and moisture, Mortierella and reducing sugar. Rhizopus, Stamphylium and Alternaria were significantly positively correlated with saccharification capacity, and Rasamsonia was significantly positively correlated with esterification capacity. This study has certain reference value for the expansion of Daqu microbiome information.

Key words： high-throughput sequencing; strong-flavor Daqu; fungal diversity; quality indicators; correlation analysis

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