为解决黄瓜低温贮藏过程中细胞膜脂过氧化作用及品质劣变的问题,采用无包装组、膜包装无孔组、微孔膜8孔组、微孔膜16孔组处理黄瓜,在4 ℃下冷藏1周后移入23 ℃下贮存1周,结合主成分分析(principal component analysis,PCA)研究微孔膜技术对黄瓜在冷藏后流通过程中细胞膜脂过氧化及品质的控制作用。结果表明,微孔膜包装组通过更好地控制包装内外气体的交换,降低呼吸速率,增强过氧化氢酶(catalase,CAT)、过氧化物酶(peroxidase,POD)、超氧化物歧化酶(superoxide dismutase,SOD)的活性,提高黄瓜的抗逆性;同时能有效减缓超氧阴离子自由基(·O-2)的产生,抑制脂氧合酶(lipoxygenase,LOX)活性,维持活性氧的代谢平衡,延缓丙二醛(malondialdehyde,MDA)的生成速率和电导率的上升,保护了细胞膜的完整性,更好地维持了黄瓜的贮藏品质。PCA较好表达出原数据的信息,可以直观地了解到各处理组品质变化趋势,得出与无包装组相比,所有包装组均能延缓黄瓜的品质下降,其中微孔膜8孔组的效果最佳。
To solve the problems of cell membrane lipid peroxidation and quality deterioration during low-temperature storage of cucumbers, cucumbers were treated with the unpackaged group, the membrane-packaged group without holes, microporous membrane group with 8 holes, and microporous membrane group with 16 holes. They were refrigerated at 4 ℃ for one week and then stored at 23 ℃ for one week. Combined with principal component analysis (PCA), the effect of microporous membrane technology on lipid peroxidation and quality control of cucumber cell membranes during circulation after refrigeration was studied. The results showed that the respiration rate was decreased and the activities of catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were enhanced in the microporous membrane packaging group by better controlling the exchange of gases inside and outside the packaging. Meanwhile, the stress resistance of cucumber was improved. In addition, it could effectively reduce the production of ·O-2, inhibit the activity of lipoxygenase (LOX), maintain the balance of active oxygen metabolism, delay the generation rate of malondialdehyde (MDA) and increase the electrical conductivity, protect the integrity of cell membrane, and better maintain the storage quality of cucumber. The principal component analysis could express the information of the original data well so that the quality change trend of each treatment group could be intuitively understood. Compared with the unpackaged group, all the packaging groups could delay the quality decline of cucumber, and the effect of the 8-well microporous membrane group was the best.
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