为了筛选出具有良好抗氧化作用和胆固醇降解能力的乳酸菌,以商业化菌株Lactobacillus rhamnosus GG为阳性对照,评价了L.kefir BNCC 190565、L.acidophilus ATCC 4356、L.buchneri BNCC 187964、L.casei CICC 23184、L.plantarum subsp. plantarum CICC 20279这5株乳酸菌的基础益生活性、羟自由基清除能力、还原力和DPPH自由基清除率、降解胆固醇能力及α -葡萄糖苷酶抑制能力。结果表明,L.acidophilus ATCC 4356和L.plantarum subsp.plantarum CICC 20279具有最强的消化道耐受力及良好的益生特性;L.acidophilus ATCC 4356、L.plantarum subsp.plantarum CICC 20279和L.casei CICC 23184具有较强的抗氧化能力;6株菌对胆固醇的降解率均在50%以上,表现出了很强的胆固醇降解能力;L.buchneri BNCC 187964的α -葡萄糖苷酶抑制率显著高于L.rhamnosus GG,其余4株菌和L.rhamnosus GG没有显著性差异。对各项指标进行综合比较,发现L.acidophilus ATCC 4356和L.plantarum subsp.plantarum CICC 20279这2株菌有着突出的性能,具有很强的应用潜力。
In order to screen out lactic acid bacteria with good antioxidant effect and cholesterol-degrading ability, Lactobacillus kefir BNCC 190565, Lactobacillus acidophilus ATCC 4356, Lactobacillus buchneri BNCC 187964, Lactobacillus casei CICC 23184, Lactobacillus plantarum subsp. plantarum CICC 20279 were evaluated with the commercial strain Lactobacillus rhamnosus GG as a positive control. The evaluation indexes included basic probiotic characteristics, hydroxyl radical scavenging ability, reducing ability and DPPH scavenging rate, cholesterol degradation ability and inhibition of α-glucosidase. The results showed that L. acidophilus ATCC 4356 and L. plantarum subsp. plantarum CICC 20279 had the best gastrointestinal tolerance and good probiotic characteristics; L. acidophilus ATCC 4356, L. plantarum subsp. plantarum CICC 20279 and L. casei CICC 23184 had better antioxidant capacity; The cholesterol degradation rate of the six strains were all above 50%, showing a strong cholesterol degradation ability; The α-glucosidase inhibition rate of L. brucei BNCC 187964 was significantly higher than that of L. rhamnosus GG, and the other four strains showed no significant difference with L. rhamnosus GG. The comprehensive comparison of various indexes indicated that L. acidophilus ATCC 4356 and L. plantarum subsp. plantarum CICC 20279 have outstanding properties and strong potential for application.
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